Sanxia Water Strider Virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KTE8|A0A0B5KTE8_9RHAB ORF2 OS=Sanxia Water Strider Virus 5 OX=1608064 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.21TSSIEE6 pKa = 4.27EE7 pKa = 3.59IWKK10 pKa = 10.31VEE12 pKa = 3.75GLEE15 pKa = 4.12KK16 pKa = 10.82VSAVTVGVTRR26 pKa = 11.84VAWSDD31 pKa = 3.35QYY33 pKa = 11.74FLSTYY38 pKa = 9.9FKK40 pKa = 10.07TIRR43 pKa = 11.84ARR45 pKa = 11.84SSATAARR52 pKa = 11.84TACRR56 pKa = 11.84VFLRR60 pKa = 11.84WYY62 pKa = 10.62VGGCLPEE69 pKa = 4.07NVKK72 pKa = 11.0LIDD75 pKa = 4.63LYY77 pKa = 11.58LSIISKK83 pKa = 9.68MSYY86 pKa = 10.78GEE88 pKa = 4.0NHH90 pKa = 6.53SLFPDD95 pKa = 3.4SGFNGATSVPIGNLGTPGTQDD116 pKa = 3.78LEE118 pKa = 4.52YY119 pKa = 10.76KK120 pKa = 9.85NNHH123 pKa = 4.55VTTLEE128 pKa = 4.24LTSSSTGAPLLWNDD142 pKa = 3.4TSVKK146 pKa = 9.15EE147 pKa = 3.79WHH149 pKa = 6.35NRR151 pKa = 11.84LLEE154 pKa = 3.95YY155 pKa = 10.53DD156 pKa = 3.62ISNSPDD162 pKa = 3.22EE163 pKa = 4.49FSEE166 pKa = 4.2SQRR169 pKa = 11.84NFVNICGFLALTMLRR184 pKa = 11.84GIAKK188 pKa = 9.8DD189 pKa = 2.91ADD191 pKa = 3.81MITRR195 pKa = 11.84SMAKK199 pKa = 8.81TVAANINNLWNLTLSEE215 pKa = 4.55CPPPNMQSIAYY226 pKa = 8.07MMTVMKK232 pKa = 10.41KK233 pKa = 10.46GSPNANLILGQILNSYY249 pKa = 8.82MDD251 pKa = 3.4QSTNPTVVGIFRR263 pKa = 11.84ASCLMSLGMNGMNSLHH279 pKa = 6.4WLMSAARR286 pKa = 11.84VNNIQPIVIARR297 pKa = 11.84CLATNSHH304 pKa = 6.29SKK306 pKa = 9.73TNTAIVDD313 pKa = 3.62LMKK316 pKa = 11.04NQMLAGQTTWEE327 pKa = 3.86WSRR330 pKa = 11.84LFNEE334 pKa = 4.84GAFMDD339 pKa = 4.24LSVRR343 pKa = 11.84EE344 pKa = 4.15DD345 pKa = 3.44PLYY348 pKa = 11.1AGTCFFLSEE357 pKa = 4.09GHH359 pKa = 7.14DD360 pKa = 3.82AQPSNYY366 pKa = 9.58LSLASYY372 pKa = 10.93LKK374 pKa = 11.04GPILDD379 pKa = 3.71SAKK382 pKa = 10.32IKK384 pKa = 10.28ALKK387 pKa = 8.81IAAYY391 pKa = 10.23LRR393 pKa = 11.84EE394 pKa = 4.12QDD396 pKa = 4.51LSTAQTTEE404 pKa = 3.39ASAVANVIIQTPTRR418 pKa = 11.84LDD420 pKa = 3.38VHH422 pKa = 6.19HH423 pKa = 6.6TAIPSLNQGAGDD435 pKa = 3.46MDD437 pKa = 4.08EE438 pKa = 4.27FF439 pKa = 6.3

Molecular weight: 48.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KXE2|A0A0B5KXE2_9RHAB ORF3 OS=Sanxia Water Strider Virus 5 OX=1608064 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 5.28LSKK5 pKa = 10.64IHH7 pKa = 6.04NQVSYY12 pKa = 10.02TKK14 pKa = 10.63VKK16 pKa = 10.4GRR18 pKa = 11.84LLMSFEE24 pKa = 4.23LTGLQSKK31 pKa = 9.12SWSVYY36 pKa = 10.15KK37 pKa = 8.77YY38 pKa = 9.97VCGLVILEE46 pKa = 4.78LFYY49 pKa = 11.62NMEE52 pKa = 3.84VSEE55 pKa = 4.56IFLDD59 pKa = 4.05SVISNLYY66 pKa = 9.74QRR68 pKa = 11.84TKK70 pKa = 10.25EE71 pKa = 4.02VPVTYY76 pKa = 10.0RR77 pKa = 11.84PSPSDD82 pKa = 3.16SLLRR86 pKa = 11.84GVVEE90 pKa = 4.79LKK92 pKa = 10.15WFTASACFPTTHH104 pKa = 7.01KK105 pKa = 10.21KK106 pKa = 10.75FNMNKK111 pKa = 9.56SRR113 pKa = 11.84TFPLISLGSQQILISYY129 pKa = 7.89DD130 pKa = 3.24FRR132 pKa = 11.84SSIEE136 pKa = 4.0PYY138 pKa = 10.63DD139 pKa = 3.94PEE141 pKa = 4.1NLIALLEE148 pKa = 4.4TGHH151 pKa = 7.6INIEE155 pKa = 3.93KK156 pKa = 10.33HH157 pKa = 6.46SADD160 pKa = 3.62LHH162 pKa = 5.98CSKK165 pKa = 10.54ARR167 pKa = 11.84KK168 pKa = 9.11QIHH171 pKa = 4.97TVRR174 pKa = 11.84KK175 pKa = 4.81TTHH178 pKa = 4.87VLNYY182 pKa = 9.92TEE184 pKa = 5.17LLNLVQMDD192 pKa = 3.52VDD194 pKa = 4.32LVKK197 pKa = 10.65AANTRR202 pKa = 11.84KK203 pKa = 10.04AEE205 pKa = 4.17KK206 pKa = 10.45NFLPTDD212 pKa = 3.79PVHH215 pKa = 6.72KK216 pKa = 9.89KK217 pKa = 10.71VEE219 pKa = 4.28VGHH222 pKa = 5.99QMLGRR227 pKa = 11.84QAPNYY232 pKa = 7.0EE233 pKa = 3.74VKK235 pKa = 9.44MRR237 pKa = 11.84KK238 pKa = 9.17GATLSKK244 pKa = 10.51SNCIVSS250 pKa = 3.43

Molecular weight: 28.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3917

250

2264

783.4

89.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.493 ± 0.737

1.557 ± 0.153

4.468 ± 0.181

5.438 ± 0.36

4.723 ± 0.532

4.468 ± 0.345

2.885 ± 0.259

7.123 ± 0.647

5.948 ± 0.802

12.076 ± 0.75

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.296

4.953 ± 0.475

5.234 ± 0.416

3.778 ± 0.339

4.187 ± 0.35

10.008 ± 0.654

6.382 ± 0.512

4.621 ± 0.616

1.583 ± 0.139

3.472 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski