Rhodovarius sp. CCP-6
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5028 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437LW05|A0A437LW05_9PROT Tyr recombinase domain-containing protein OS=Rhodovarius sp. CCP-6 OX=1979269 GN=EOD42_25040 PE=4 SV=1
MM1 pKa = 7.4 AVEE4 pKa = 4.81 LSDD7 pKa = 4.96 GGYY10 pKa = 8.31 DD11 pKa = 3.88 TAWVSANGWTMSYY24 pKa = 10.46 GVEE27 pKa = 3.88 AGRR30 pKa = 11.84 LIGTATEE37 pKa = 4.21 LTGNALDD44 pKa = 3.95 NVLVANADD52 pKa = 3.68 FASAIHH58 pKa = 6.4 GGGGNDD64 pKa = 4.52 EE65 pKa = 4.13 IWGSNQNGDD74 pKa = 4.05 VLDD77 pKa = 4.68 GGAGDD82 pKa = 3.83 DD83 pKa = 3.81 VLRR86 pKa = 11.84 GGSGVTTFIGGEE98 pKa = 4.13 GNDD101 pKa = 3.12 QFVVNNVNSVIVEE114 pKa = 4.11 KK115 pKa = 10.74 AGEE118 pKa = 4.65 GIDD121 pKa = 4.01 TAWVSADD128 pKa = 2.78 GWHH131 pKa = 6.41 VSDD134 pKa = 3.46 NVEE137 pKa = 3.84 IVRR140 pKa = 11.84 LFGAAHH146 pKa = 5.69 SVVLGTSGAQVVANASGSTITAQAGDD172 pKa = 3.73 NVFWGQGGNDD182 pKa = 3.57 VFIGGAGNDD191 pKa = 3.1 IFYY194 pKa = 10.51 SGTGVTQMFGGAGNDD209 pKa = 3.23 HH210 pKa = 7.2 FIIKK214 pKa = 9.84 NAADD218 pKa = 3.56 TVVEE222 pKa = 4.22 NANGGYY228 pKa = 7.93 DD229 pKa = 3.81 TAWLEE234 pKa = 4.04 VSGWTVAEE242 pKa = 4.13 NVEE245 pKa = 4.15 VAYY248 pKa = 10.89 LSGTANSITGNASGTNLVANSTMASALTAGTGFTIFWGSNYY289 pKa = 9.76 GDD291 pKa = 3.56 TMKK294 pKa = 10.49 IGAGGGNVYY303 pKa = 10.49 GYY305 pKa = 11.01 GGADD309 pKa = 2.95 TFEE312 pKa = 5.35 FGPHH316 pKa = 5.76 WGLTQVADD324 pKa = 4.52 FNHH327 pKa = 6.94 AEE329 pKa = 4.04 GDD331 pKa = 3.71 KK332 pKa = 11.2 LDD334 pKa = 4.76 FSASGLSMADD344 pKa = 3.38 LNVTNYY350 pKa = 10.32 SDD352 pKa = 3.33 KK353 pKa = 11.13 TLIEE357 pKa = 4.47 HH358 pKa = 6.54 NGEE361 pKa = 3.95 QIILYY366 pKa = 10.05 GVTSLQASDD375 pKa = 4.45 FVFF378 pKa = 4.18
Molecular weight: 38.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.897
Patrickios 1.189
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A437MF02|A0A437MF02_9PROT Uncharacterized protein OS=Rhodovarius sp. CCP-6 OX=1979269 GN=EOD42_14065 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPSKK9 pKa = 8.8 IVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.55 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NVLAARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.85 GRR39 pKa = 11.84 SKK41 pKa = 11.16 LSAA44 pKa = 3.74
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5028
0
5028
1623387
33
3309
322.9
34.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.091 ± 0.054
0.841 ± 0.011
4.952 ± 0.025
5.566 ± 0.03
3.358 ± 0.019
9.262 ± 0.038
2.064 ± 0.017
4.331 ± 0.022
2.187 ± 0.025
10.62 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.688 ± 0.018
2.352 ± 0.024
6.147 ± 0.031
3.22 ± 0.021
7.938 ± 0.04
4.733 ± 0.023
5.119 ± 0.029
7.176 ± 0.027
1.5 ± 0.014
1.854 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here