Erysipelatoclostridium sp. AM42-17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelatoclostridium; unclassified Erysipelatoclostridium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417CC36|A0A417CC36_9FIRM Ribosomal RNA small subunit methyltransferase H OS=Erysipelatoclostridium sp. AM42-17 OX=2293102 GN=rsmH PE=3 SV=1
MM1 pKa = 7.7AKK3 pKa = 8.49ITVNEE8 pKa = 3.89NCIGCGACTGVAPEE22 pKa = 4.3TFEE25 pKa = 5.68IGDD28 pKa = 4.33DD29 pKa = 3.95GLAKK33 pKa = 10.52AINEE37 pKa = 3.93DD38 pKa = 3.54VEE40 pKa = 4.48AGKK43 pKa = 9.75EE44 pKa = 3.84AAEE47 pKa = 4.22TCPVEE52 pKa = 4.83AIEE55 pKa = 4.7VEE57 pKa = 4.47EE58 pKa = 4.14

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417CJC8|A0A417CJC8_9FIRM DUF4430 domain-containing protein OS=Erysipelatoclostridium sp. AM42-17 OX=2293102 GN=DW911_02260 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.34VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2067

0

2067

640331

23

1874

309.8

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.666 ± 0.051

1.426 ± 0.019

6.07 ± 0.044

6.417 ± 0.058

4.25 ± 0.043

6.105 ± 0.051

2.469 ± 0.029

8.782 ± 0.059

8.121 ± 0.044

9.373 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.951 ± 0.027

5.221 ± 0.04

2.841 ± 0.028

4.156 ± 0.041

3.263 ± 0.04

5.762 ± 0.04

5.048 ± 0.048

6.601 ± 0.04

0.644 ± 0.017

4.835 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski