Enterococcus phage vB_EfaP_Zip

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Sarlesvirinae; Minhovirus; Enterococcus virus Zip

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411B6V0|A0A411B6V0_9CAUD HNH endonuclease OS=Enterococcus phage vB_EfaP_Zip OX=2501743 GN=Zip_13 PE=4 SV=1
MM1 pKa = 7.72NKK3 pKa = 9.68FLWVLNAFLWTCVIIAWTMFFTAPIEE29 pKa = 4.09RR30 pKa = 11.84EE31 pKa = 4.02NEE33 pKa = 3.8NEE35 pKa = 4.08QITSITTQQSEE46 pKa = 4.15ISSTTEE52 pKa = 3.27EE53 pKa = 4.28TQATTASTTYY63 pKa = 8.42QTEE66 pKa = 4.15STTQEE71 pKa = 4.2TVTTPVYY78 pKa = 10.04TPANTEE84 pKa = 3.96EE85 pKa = 4.44YY86 pKa = 10.27TVGNYY91 pKa = 8.44MGYY94 pKa = 10.55DD95 pKa = 3.43PNLQGEE101 pKa = 4.96SYY103 pKa = 10.75QITRR107 pKa = 11.84NSDD110 pKa = 3.09GVKK113 pKa = 10.46GVITIDD119 pKa = 3.46SQANMQWYY127 pKa = 9.74YY128 pKa = 10.93YY129 pKa = 9.4DD130 pKa = 3.63ASGIHH135 pKa = 5.57TNGRR139 pKa = 11.84GG140 pKa = 3.12

Molecular weight: 15.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411B6T4|A0A411B6T4_9CAUD DNA-directed DNA polymerase OS=Enterococcus phage vB_EfaP_Zip OX=2501743 GN=Zip_17 PE=4 SV=1
MM1 pKa = 6.48ITKK4 pKa = 10.14GIAGRR9 pKa = 11.84RR10 pKa = 11.84QGKK13 pKa = 8.92VKK15 pKa = 10.49GVVIHH20 pKa = 6.88NDD22 pKa = 3.06AGSNGATCSFYY33 pKa = 10.65RR34 pKa = 11.84GYY36 pKa = 9.44LTDD39 pKa = 4.56ANAANGFAHH48 pKa = 7.68WYY50 pKa = 8.59LASDD54 pKa = 5.32GILQVEE60 pKa = 5.02DD61 pKa = 3.75EE62 pKa = 5.58ANMAWHH68 pKa = 6.77TANSDD73 pKa = 3.29GNANFIGIEE82 pKa = 4.1ACQSMGNLAQFLDD95 pKa = 3.64NEE97 pKa = 3.96KK98 pKa = 10.61RR99 pKa = 11.84AIKK102 pKa = 10.05LAAEE106 pKa = 3.69ILKK109 pKa = 10.52RR110 pKa = 11.84HH111 pKa = 5.59GLQPNTTTVRR121 pKa = 11.84LHH123 pKa = 5.57NTFSSTACPHH133 pKa = 7.01RR134 pKa = 11.84SQEE137 pKa = 3.87QHH139 pKa = 5.55GAGSKK144 pKa = 7.22TQAYY148 pKa = 8.86FIAEE152 pKa = 4.37IKK154 pKa = 10.51KK155 pKa = 9.86HH156 pKa = 5.49MNGSTPAPAKK166 pKa = 9.95PSTPKK171 pKa = 10.72GHH173 pKa = 7.29DD174 pKa = 3.27EE175 pKa = 3.97AVAASKK181 pKa = 10.42PKK183 pKa = 10.05HH184 pKa = 6.03QGNAWGKK191 pKa = 7.85LDD193 pKa = 4.24KK194 pKa = 11.04YY195 pKa = 10.51RR196 pKa = 11.84EE197 pKa = 4.07EE198 pKa = 3.84PAGQIRR204 pKa = 11.84VAGWLVPDD212 pKa = 4.95KK213 pKa = 10.62PQGPIGKK220 pKa = 8.56YY221 pKa = 10.04AYY223 pKa = 10.55VLFMQHH229 pKa = 5.23GTGKK233 pKa = 10.24EE234 pKa = 3.83LTRR237 pKa = 11.84VQSQGIKK244 pKa = 10.39RR245 pKa = 11.84PDD247 pKa = 3.03VKK249 pKa = 10.47KK250 pKa = 10.61AYY252 pKa = 9.67GYY254 pKa = 10.55QGGQEE259 pKa = 4.31LGFDD263 pKa = 3.76VTVKK267 pKa = 10.47KK268 pKa = 10.75AQFKK272 pKa = 9.54GKK274 pKa = 9.98KK275 pKa = 8.93VDD277 pKa = 3.93VILRR281 pKa = 11.84RR282 pKa = 11.84ANKK285 pKa = 10.14SNGEE289 pKa = 3.9GSVNDD294 pKa = 3.39VRR296 pKa = 11.84IDD298 pKa = 4.0SIYY301 pKa = 10.17LTLL304 pKa = 4.81

Molecular weight: 33.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

5986

50

779

272.1

30.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.131 ± 0.585

0.685 ± 0.119

5.981 ± 0.247

7.133 ± 0.588

4.577 ± 0.451

6.031 ± 0.499

1.971 ± 0.223

6.482 ± 0.308

7.384 ± 0.407

7.935 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.168

7.384 ± 0.5

3.542 ± 0.306

4.093 ± 0.39

3.558 ± 0.348

5.847 ± 0.27

7.183 ± 0.464

5.68 ± 0.332

1.203 ± 0.125

4.878 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski