Streptococcus satellite phage Javan734
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZVE4|A0A4D5ZVE4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan734 OX=2558828 GN=JavanS734_0019 PE=4 SV=1
MM1 pKa = 7.22 TLDD4 pKa = 4.01 LGKK7 pKa = 8.39 MTQAEE12 pKa = 4.45 FDD14 pKa = 3.72 EE15 pKa = 4.66 VMADD19 pKa = 3.21 IKK21 pKa = 11.13 ARR23 pKa = 11.84 NPNLLQFITDD33 pKa = 3.81 LLDD36 pKa = 3.75 RR37 pKa = 11.84 KK38 pKa = 8.57 VTPEE42 pKa = 4.1 EE43 pKa = 4.0 VDD45 pKa = 3.88 DD46 pKa = 4.43 LLNMEE51 pKa = 4.04 QAEE54 pKa = 4.0 QVEE57 pKa = 4.75 YY58 pKa = 10.8 IEE60 pKa = 5.26 NYY62 pKa = 7.17 QAKK65 pKa = 10.08 AA66 pKa = 3.19
Molecular weight: 7.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.367
IPC2_protein 4.088
IPC_protein 3.973
Toseland 3.795
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.884
Nozaki 4.075
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.088
Patrickios 3.859
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 4.015
Protein with the highest isoelectric point:
>tr|A0A4D5ZVD4|A0A4D5ZVD4_9VIRU Glycerate kinase OS=Streptococcus satellite phage Javan734 OX=2558828 GN=JavanS734_0009 PE=4 SV=1
MM1 pKa = 7.53 RR2 pKa = 11.84 CEE4 pKa = 4.37 MMPKK8 pKa = 9.9 SNYY11 pKa = 9.57 RR12 pKa = 11.84 EE13 pKa = 3.74 MRR15 pKa = 11.84 FISSLGGKK23 pKa = 9.41 LLATRR28 pKa = 11.84 ILFTVTIANSQQ39 pKa = 3.1
Molecular weight: 4.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.545
IPC_protein 10.131
Toseland 10.467
ProMoST 10.116
Dawson 10.584
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.877
Grimsley 10.643
Solomon 10.701
Lehninger 10.672
Nozaki 10.482
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.804
IPC_peptide 10.701
IPC2_peptide 9.399
IPC2.peptide.svr19 8.455
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22
0
22
3069
39
507
139.5
16.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.442 ± 0.46
0.978 ± 0.209
5.409 ± 0.364
7.983 ± 0.556
4.301 ± 0.54
4.725 ± 0.532
1.857 ± 0.27
7.429 ± 0.423
9.677 ± 0.608
10.231 ± 0.604
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.639 ± 0.426
5.767 ± 0.405
3.128 ± 0.4
3.975 ± 0.336
4.888 ± 0.383
5.539 ± 0.491
5.474 ± 0.49
5.213 ± 0.399
0.912 ± 0.19
4.431 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here