Phytophthora rubi
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 35379 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A4C5U4|A0A6A4C5U4_9STRA Uncharacterized protein OS=Phytophthora rubi OX=129364 GN=PR002_g26572 PE=4 SV=1
MM1 pKa = 7.44 TFLILEE7 pKa = 4.28 NSSYY11 pKa = 9.77 STSSQQPVYY20 pKa = 10.81 LCVDD24 pKa = 3.74 MNIHH28 pKa = 6.83 ALLLVAASMTASATAKK44 pKa = 9.17 FTVTSGTTEE53 pKa = 3.83 ATTSTTEE60 pKa = 3.39 NVAFVKK66 pKa = 10.31 QWTLNSATTADD77 pKa = 4.32 TIDD80 pKa = 5.43 SIDD83 pKa = 4.08 LDD85 pKa = 4.04 LAGRR89 pKa = 11.84 VYY91 pKa = 10.86 VSYY94 pKa = 11.41 VSGLPSGVLGYY105 pKa = 11.23 VNVSGDD111 pKa = 3.4 SQTVVDD117 pKa = 5.39 AVTVINDD124 pKa = 3.96 DD125 pKa = 5.31 ANDD128 pKa = 3.77 TDD130 pKa = 6.41 DD131 pKa = 6.07 NDD133 pKa = 4.24 TNDD136 pKa = 3.84 NDD138 pKa = 3.95 GEE140 pKa = 4.59 LNVRR144 pKa = 11.84 IGNSTSTATTLSGYY158 pKa = 10.66 LLTEE162 pKa = 5.02 IILASSGVVTDD173 pKa = 3.82 VKK175 pKa = 10.3 SQRR178 pKa = 11.84 SAQVVVEE185 pKa = 4.38 DD186 pKa = 4.42 GVLVSSSTTAEE197 pKa = 4.15 LQVEE201 pKa = 4.55 ASGSSAVYY209 pKa = 10.06 VSAASTAVSVRR220 pKa = 11.84 QLQLDD225 pKa = 3.82 AAGTAQLQFNVKK237 pKa = 9.39 SVSVTDD243 pKa = 3.57 EE244 pKa = 4.16 AQFEE248 pKa = 4.51 AQGSAVVSVLASSLEE263 pKa = 3.79 ASQLDD268 pKa = 4.89 LEE270 pKa = 4.65 TQSTGTICISAQQVTATNYY289 pKa = 9.59 QGQDD293 pKa = 2.86 ASRR296 pKa = 11.84 ISMPNASSKK305 pKa = 10.83 YY306 pKa = 10.21 DD307 pKa = 3.49 STGSLACDD315 pKa = 3.39 EE316 pKa = 4.63 SSIPTRR322 pKa = 11.84 EE323 pKa = 4.0 PSCVSSACASSSTSGTSTTATVVTSAPTTTNDD355 pKa = 4.33 DD356 pKa = 4.02 DD357 pKa = 6.48 ASDD360 pKa = 4.49 DD361 pKa = 4.26 SNASSTPRR369 pKa = 11.84 LAALVVAVIGVVTAGLLL386 pKa = 3.53
Molecular weight: 39.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.35
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.062
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.808
Patrickios 1.125
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A6A4BL47|A0A6A4BL47_9STRA Chromo domain-containing protein OS=Phytophthora rubi OX=129364 GN=PR003_g29016 PE=4 SV=1
MM1 pKa = 7.77 SSQKK5 pKa = 10.26 KK6 pKa = 10.05 SKK8 pKa = 10.72 SQMKK12 pKa = 9.72 PKK14 pKa = 10.12 SQRR17 pKa = 11.84 LSSRR21 pKa = 11.84 KK22 pKa = 8.32 KK23 pKa = 8.07 SKK25 pKa = 9.78 RR26 pKa = 11.84 RR27 pKa = 11.84 KK28 pKa = 7.38 VSKK31 pKa = 9.92 RR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 8.37 SKK36 pKa = 10.78 RR37 pKa = 11.84 KK38 pKa = 8.0 ISKK41 pKa = 9.53 SQVVTT46 pKa = 3.85
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.111
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.808
Grimsley 12.852
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.515
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
35064
315
35379
12151655
10
10508
343.5
38.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.602 ± 0.015
1.629 ± 0.008
5.819 ± 0.01
6.625 ± 0.015
3.464 ± 0.009
6.205 ± 0.015
2.321 ± 0.006
3.673 ± 0.01
4.909 ± 0.016
9.097 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.006
3.212 ± 0.008
5.112 ± 0.014
4.157 ± 0.01
6.791 ± 0.016
8.151 ± 0.017
5.907 ± 0.014
7.181 ± 0.014
1.277 ± 0.004
2.472 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here