Rhodobacteraceae bacterium 2CG4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L5YZW5|A0A6L5YZW5_9RHOB Ubiquinone biosynthesis O-methyltransferase OS=Rhodobacteraceae bacterium 2CG4 OX=2662447 GN=ubiG PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.98RR3 pKa = 11.84HH4 pKa = 6.72LIALSAVFAAATPAFAGGLAPAPIDD29 pKa = 3.54QPVIAPAPVPVAPLGVDD46 pKa = 2.8WTGPYY51 pKa = 10.16VGAQLGYY58 pKa = 11.1GFGDD62 pKa = 3.55ADD64 pKa = 3.6GTDD67 pKa = 3.78FDD69 pKa = 4.89GVLGGAQAGYY79 pKa = 10.83NYY81 pKa = 10.72DD82 pKa = 3.33LGKK85 pKa = 9.77WVVGAEE91 pKa = 3.89LDD93 pKa = 3.88YY94 pKa = 11.8NFANLEE100 pKa = 4.19LDD102 pKa = 3.8GSSDD106 pKa = 3.79TIDD109 pKa = 3.09QIGRR113 pKa = 11.84LKK115 pKa = 10.89AKK117 pKa = 10.24AGYY120 pKa = 9.61EE121 pKa = 3.6IGKK124 pKa = 9.89ALLYY128 pKa = 8.89GTAGGAYY135 pKa = 10.08AEE137 pKa = 4.47ADD139 pKa = 3.56VGGSTLSDD147 pKa = 3.87FGWVAGAGVDD157 pKa = 4.75YY158 pKa = 10.4MVTDD162 pKa = 4.23NIIAGVEE169 pKa = 3.95YY170 pKa = 9.99LYY172 pKa = 11.0HH173 pKa = 7.34SFDD176 pKa = 4.68DD177 pKa = 4.59FDD179 pKa = 6.24DD180 pKa = 4.41SGVDD184 pKa = 3.31VDD186 pKa = 4.89ANTVAAKK193 pKa = 10.65VSFKK197 pKa = 10.93FF198 pKa = 3.65

Molecular weight: 20.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L5Z6F2|A0A6L5Z6F2_9RHOB Type II toxin-antitoxin system RelE/ParE family toxin OS=Rhodobacteraceae bacterium 2CG4 OX=2662447 GN=GE300_21600 PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.12RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 9.18RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 8.78ILNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AQGRR40 pKa = 11.84SRR42 pKa = 11.84LSAA45 pKa = 3.73

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4349

0

4349

1366446

18

2814

314.2

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.953 ± 0.073

0.89 ± 0.013

6.039 ± 0.035

5.553 ± 0.034

3.525 ± 0.022

9.092 ± 0.033

2.048 ± 0.021

4.632 ± 0.03

2.288 ± 0.028

10.343 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.016

2.171 ± 0.021

5.567 ± 0.029

2.936 ± 0.02

7.909 ± 0.04

4.561 ± 0.025

5.105 ± 0.025

7.378 ± 0.03

1.424 ± 0.016

2.069 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski