Rasamsonia emersonii CBS 393.64
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9842 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F4Z3Q1|A0A0F4Z3Q1_TALEM WD repeat protein OS=Rasamsonia emersonii CBS 393.64 OX=1408163 GN=T310_0832 PE=4 SV=1
MM1 pKa = 7.55 PGIYY5 pKa = 9.66 TLVTLSTLLTSINVLAAPAVPATDD29 pKa = 4.3 APAPTPPPKK38 pKa = 10.42 LEE40 pKa = 4.07 EE41 pKa = 4.1 RR42 pKa = 11.84 ATTCTFSGSDD52 pKa = 3.63 GASSASKK59 pKa = 10.75 SKK61 pKa = 9.53 TSCSTIVLSDD71 pKa = 3.44 VAVPSGTTLDD81 pKa = 3.9 LTNLNDD87 pKa = 3.64 GTHH90 pKa = 6.34 VIFEE94 pKa = 4.49 GEE96 pKa = 4.12 TTFGYY101 pKa = 10.01 EE102 pKa = 3.84 EE103 pKa = 4.09 WSGPLVSVSGTDD115 pKa = 3.13 ITVTAASGATLNGDD129 pKa = 4.09 GSRR132 pKa = 11.84 WWDD135 pKa = 3.42 GEE137 pKa = 3.87 GSNGGKK143 pKa = 8.26 TKK145 pKa = 10.59 PKK147 pKa = 10.16 FFYY150 pKa = 10.65 AHH152 pKa = 6.24 NLKK155 pKa = 10.56 LSSISGLYY163 pKa = 8.66 IQNSPVQVFSISGSEE178 pKa = 4.32 DD179 pKa = 3.04 LTLSDD184 pKa = 3.91 ITIDD188 pKa = 5.54 DD189 pKa = 3.95 SAGDD193 pKa = 3.59 NAGAANTDD201 pKa = 3.3 GFDD204 pKa = 3.34 VGEE207 pKa = 4.16 SSGIVITGATVYY219 pKa = 10.94 NQDD222 pKa = 3.4 DD223 pKa = 4.15 CLAVNSGTNITFSGGLCSGGHH244 pKa = 5.81 GLSIGSVGGRR254 pKa = 11.84 SDD256 pKa = 3.49 NTVSDD261 pKa = 3.72 VTIEE265 pKa = 3.95 SSQVKK270 pKa = 10.05 NSQNGVRR277 pKa = 11.84 IKK279 pKa = 9.73 TVYY282 pKa = 10.35 DD283 pKa = 3.27 ATGSVSGVTYY293 pKa = 10.99 KK294 pKa = 10.92 DD295 pKa = 3.0 ITLSGITDD303 pKa = 3.35 YY304 pKa = 11.65 GIVVRR309 pKa = 11.84 QDD311 pKa = 3.47 YY312 pKa = 11.2 EE313 pKa = 4.21 NGSPTGTPTNGVPISDD329 pKa = 3.64 FTLDD333 pKa = 3.63 NVQGTVDD340 pKa = 4.09 SDD342 pKa = 3.41 ATDD345 pKa = 3.17 IFILCGAGSCSDD357 pKa = 3.6 WTWTDD362 pKa = 3.28 VNVTGGKK369 pKa = 10.1 VSDD372 pKa = 4.03 DD373 pKa = 4.12 CEE375 pKa = 4.19 NVPSGISCSAA385 pKa = 3.3
Molecular weight: 39.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.656
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.846
Rodwell 3.706
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.706
EMBOSS 3.846
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A0F4YHL6|A0A0F4YHL6_TALEM Protein kinase (Fragment) OS=Rasamsonia emersonii CBS 393.64 OX=1408163 GN=T310_8391 PE=4 SV=1
QQ1 pKa = 7.11 GLVSRR6 pKa = 11.84 LKK8 pKa = 10.62 CPRR11 pKa = 11.84 PRR13 pKa = 11.84 PRR15 pKa = 11.84 PRR17 pKa = 11.84 LKK19 pKa = 10.39 RR20 pKa = 11.84 APTRR24 pKa = 11.84 LPTRR28 pKa = 11.84 HH29 pKa = 5.94 SPRR32 pKa = 11.84 SRR34 pKa = 11.84 MVEE37 pKa = 3.78 LPSRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 LLRR46 pKa = 11.84 PRR48 pKa = 11.84 ARR50 pKa = 11.84 KK51 pKa = 9.64 SLGSLSPTKK60 pKa = 10.37 RR61 pKa = 11.84 KK62 pKa = 9.7 LLARR66 pKa = 11.84 TGRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 GRR73 pKa = 11.84 LLAAPNLNRR82 pKa = 11.84 TATRR86 pKa = 11.84 RR87 pKa = 11.84 WPAA90 pKa = 2.86
Molecular weight: 10.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.096
IPC_protein 12.705
Toseland 12.866
ProMoST 13.364
Dawson 12.866
Bjellqvist 12.866
Wikipedia 13.349
Rodwell 12.457
Grimsley 12.91
Solomon 13.364
Lehninger 13.261
Nozaki 12.866
DTASelect 12.866
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.164
IPC_peptide 13.364
IPC2_peptide 12.354
IPC2.peptide.svr19 9.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9842
0
9842
4794008
73
7865
487.1
53.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.564 ± 0.021
1.255 ± 0.009
5.662 ± 0.017
6.324 ± 0.027
3.589 ± 0.016
6.649 ± 0.018
2.365 ± 0.01
4.743 ± 0.016
4.701 ± 0.021
8.92 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.961 ± 0.008
3.647 ± 0.013
6.28 ± 0.027
4.138 ± 0.019
6.604 ± 0.023
8.557 ± 0.03
5.782 ± 0.016
6.109 ± 0.018
1.403 ± 0.009
2.745 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here