Sinorhizobium phage phiN3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Emdodecavirus; Sinorhizobium virus N3

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6SJ21|A0A0F6SJ21_9CAUD Appr-1-p processing protein OS=Sinorhizobium phage phiN3 OX=1647405 GN=PHIN3_180 PE=4 SV=1
MM1 pKa = 7.36TEE3 pKa = 3.56EE4 pKa = 4.24TTRR7 pKa = 11.84KK8 pKa = 9.23PSAVLYY14 pKa = 10.6LYY16 pKa = 10.44DD17 pKa = 5.58DD18 pKa = 4.26PTDD21 pKa = 3.55EE22 pKa = 4.58LQYY25 pKa = 8.58TVEE28 pKa = 4.01WEE30 pKa = 4.22FVDD33 pKa = 5.08VSGNDD38 pKa = 3.52KK39 pKa = 10.94ASSSHH44 pKa = 6.03IALGAIIHH52 pKa = 6.71AFQNGEE58 pKa = 4.02LDD60 pKa = 4.15HH61 pKa = 7.18IINTFADD68 pKa = 3.82YY69 pKa = 10.99AIATLTPDD77 pKa = 4.02DD78 pKa = 4.21VEE80 pKa = 4.22

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6YPC3|A0A0F6YPC3_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiN3 OX=1647405 GN=PHIN3_126 PE=4 SV=1
MM1 pKa = 7.59PVNRR5 pKa = 11.84DD6 pKa = 2.81KK7 pKa = 11.4AAADD11 pKa = 4.41LYY13 pKa = 10.04RR14 pKa = 11.84VSVRR18 pKa = 11.84GARR21 pKa = 11.84KK22 pKa = 9.17EE23 pKa = 3.78MIMFSQCDD31 pKa = 3.33EE32 pKa = 4.09GRR34 pKa = 11.84DD35 pKa = 3.66VGLLRR40 pKa = 11.84AYY42 pKa = 7.76MASDD46 pKa = 3.35QAQLARR52 pKa = 11.84IRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84NAVTRR61 pKa = 11.84GDD63 pKa = 3.59KK64 pKa = 10.66VKK66 pKa = 10.79RR67 pKa = 11.84KK68 pKa = 9.51ADD70 pKa = 4.8FYY72 pKa = 11.71AVLARR77 pKa = 11.84MAEE80 pKa = 3.96RR81 pKa = 11.84LDD83 pKa = 3.83RR84 pKa = 11.84LTCGFF89 pKa = 4.2

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

402

0

402

63799

31

1802

158.7

18.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.439 ± 0.2

0.969 ± 0.062

6.547 ± 0.112

7.248 ± 0.212

4.741 ± 0.092

6.293 ± 0.16

2.022 ± 0.094

6.441 ± 0.119

6.597 ± 0.205

7.522 ± 0.138

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.1

4.914 ± 0.154

3.644 ± 0.103

2.851 ± 0.072

5.321 ± 0.134

5.931 ± 0.196

6.318 ± 0.264

6.723 ± 0.131

1.481 ± 0.072

4.155 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski