Hubei picorna-like virus 67

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KM14|A0A1L3KM14_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 67 OX=1923150 PE=4 SV=1
MM1 pKa = 7.82EE2 pKa = 5.31EE3 pKa = 4.43SQDD6 pKa = 3.76QSWGVPVFQPAGLTEE21 pKa = 4.32LSLRR25 pKa = 11.84QISLPTFNEE34 pKa = 4.02DD35 pKa = 3.85QIPPQSFAQEE45 pKa = 3.75RR46 pKa = 11.84RR47 pKa = 11.84EE48 pKa = 3.92LVAQRR53 pKa = 11.84VQLDD57 pKa = 3.63PSLAWAHH64 pKa = 4.97THH66 pKa = 6.71LVAAYY71 pKa = 9.64HH72 pKa = 6.94RR73 pKa = 11.84DD74 pKa = 3.34FNLISNQALMVRR86 pKa = 11.84DD87 pKa = 4.35LCQEE91 pKa = 4.0TVEE94 pKa = 5.7ILAEE98 pKa = 3.84WEE100 pKa = 3.93TRR102 pKa = 11.84MSHH105 pKa = 5.27FRR107 pKa = 11.84KK108 pKa = 9.46IQEE111 pKa = 3.99SQKK114 pKa = 10.84RR115 pKa = 11.84SIAPLSRR122 pKa = 11.84MGLQIAQNMSALICRR137 pKa = 11.84LRR139 pKa = 11.84AKK141 pKa = 9.89QLPPGIQEE149 pKa = 4.16SKK151 pKa = 11.01VLLRR155 pKa = 11.84SLNILLRR162 pKa = 11.84SYY164 pKa = 11.04HH165 pKa = 6.95RR166 pKa = 11.84ILTKK170 pKa = 9.7MCLEE174 pKa = 4.44VSRR177 pKa = 11.84HH178 pKa = 5.35PKK180 pKa = 9.97VEE182 pKa = 3.67IDD184 pKa = 3.51SRR186 pKa = 11.84EE187 pKa = 3.82QALLSLEE194 pKa = 4.31LAHH197 pKa = 6.78CLVILAGEE205 pKa = 4.33SEE207 pKa = 4.56AIEE210 pKa = 4.42YY211 pKa = 10.04YY212 pKa = 10.38HH213 pKa = 6.93LEE215 pKa = 4.03KK216 pKa = 10.28MPEE219 pKa = 3.98VLEE222 pKa = 4.16VEE224 pKa = 4.38PVHH227 pKa = 6.93LNRR230 pKa = 11.84KK231 pKa = 8.77KK232 pKa = 10.65VLAGALQYY240 pKa = 10.68YY241 pKa = 10.08RR242 pKa = 11.84SLLLGQDD249 pKa = 3.71FQSVHH254 pKa = 6.33LSLEE258 pKa = 4.27LEE260 pKa = 4.33SLSMLPKK267 pKa = 9.97IEE269 pKa = 4.36SKK271 pKa = 11.44DD272 pKa = 3.63NITLIWRR279 pKa = 11.84ALRR282 pKa = 11.84HH283 pKa = 5.78NKK285 pKa = 8.35WATLTSSLQEE295 pKa = 4.15FLMPPHH301 pKa = 6.39GPEE304 pKa = 4.0PRR306 pKa = 11.84SDD308 pKa = 3.25RR309 pKa = 11.84YY310 pKa = 10.45LFKK313 pKa = 10.62ISEE316 pKa = 4.18FFPFKK321 pKa = 10.75FFDD324 pKa = 4.03LSQGMLFHH332 pKa = 6.73VVRR335 pKa = 11.84NVQSNHH341 pKa = 3.47VHH343 pKa = 6.15AGEE346 pKa = 3.78IAKK349 pKa = 10.18YY350 pKa = 9.87FSKK353 pKa = 10.74LLKK356 pKa = 10.6EE357 pKa = 4.34EE358 pKa = 4.34IEE360 pKa = 4.06LDD362 pKa = 3.74VNVFEE367 pKa = 4.7TLFGFIIQDD376 pKa = 3.64LQHH379 pKa = 6.72FDD381 pKa = 2.77TWYY384 pKa = 8.59VTIDD388 pKa = 3.52FPRR391 pKa = 11.84QVFDD395 pKa = 4.59LDD397 pKa = 3.77FDD399 pKa = 4.72VVDD402 pKa = 3.57SWRR405 pKa = 11.84TSTLEE410 pKa = 3.78QRR412 pKa = 11.84RR413 pKa = 11.84PDD415 pKa = 4.17DD416 pKa = 4.2GDD418 pKa = 4.68EE419 pKa = 3.94VDD421 pKa = 3.88HH422 pKa = 6.73WFTSRR427 pKa = 11.84IQSDD431 pKa = 3.2MDD433 pKa = 3.45FEE435 pKa = 4.49RR436 pKa = 11.84VMEE439 pKa = 4.36RR440 pKa = 11.84FHH442 pKa = 8.03NPFMLLEE449 pKa = 4.1PQFINLQINQQLINLSYY466 pKa = 9.05TLCSRR471 pKa = 11.84CQVRR475 pKa = 11.84FLNRR479 pKa = 11.84PGAPPNSEE487 pKa = 3.85DD488 pKa = 4.33ALYY491 pKa = 10.72CDD493 pKa = 4.04RR494 pKa = 11.84CRR496 pKa = 11.84NPSFRR501 pKa = 4.26

Molecular weight: 58.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KM14|A0A1L3KM14_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 67 OX=1923150 PE=4 SV=1
MM1 pKa = 7.56ALFAAEE7 pKa = 4.67EE8 pKa = 4.09ILTTGLRR15 pKa = 11.84PHH17 pKa = 7.25KK18 pKa = 10.16IINNVVRR25 pKa = 11.84PKK27 pKa = 10.23EE28 pKa = 4.13RR29 pKa = 11.84PPPPPNGRR37 pKa = 11.84KK38 pKa = 9.44SGSVLGSAGFSTSWADD54 pKa = 3.57RR55 pKa = 11.84AQFEE59 pKa = 4.78TDD61 pKa = 3.52FVTDD65 pKa = 3.0VQRR68 pKa = 11.84RR69 pKa = 11.84SNTSTILRR77 pKa = 11.84SRR79 pKa = 11.84EE80 pKa = 3.95KK81 pKa = 9.42GTSSATSSIRR91 pKa = 11.84SVSGMGSYY99 pKa = 10.76ASSRR103 pKa = 11.84SLPSRR108 pKa = 11.84FQPDD112 pKa = 3.95FKK114 pKa = 11.18SGFDD118 pKa = 3.5GARR121 pKa = 11.84SVSRR125 pKa = 11.84NSRR128 pKa = 11.84NSSRR132 pKa = 11.84MGDD135 pKa = 3.71SNVSLQEE142 pKa = 3.8NSGVAEE148 pKa = 5.39AINSSIVTHH157 pKa = 6.71GIADD161 pKa = 3.78SSEE164 pKa = 4.27HH165 pKa = 5.93VSADD169 pKa = 3.56LPSPGQAIATGDD181 pKa = 3.55TGVEE185 pKa = 4.51SIAEE189 pKa = 4.17EE190 pKa = 4.4PEE192 pKa = 3.79HH193 pKa = 7.03LITFISSYY201 pKa = 6.78THH203 pKa = 6.32EE204 pKa = 4.56NVPGSEE210 pKa = 4.29SSSEE214 pKa = 4.04SGDD217 pKa = 3.61RR218 pKa = 11.84QSRR221 pKa = 11.84ASTPVSRR228 pKa = 11.84ARR230 pKa = 11.84TLLSNISGRR239 pKa = 11.84IGGNRR244 pKa = 11.84VLPSGEE250 pKa = 4.01NARR253 pKa = 11.84SAGSRR258 pKa = 11.84TGASKK263 pKa = 10.69SKK265 pKa = 9.5EE266 pKa = 3.69GSRR269 pKa = 11.84RR270 pKa = 11.84GPSVLSEE277 pKa = 4.14FAAGAGFSVGPLVSGIGEE295 pKa = 4.61SINAAKK301 pKa = 10.2DD302 pKa = 3.34RR303 pKa = 11.84KK304 pKa = 9.43QRR306 pKa = 11.84QHH308 pKa = 5.98NLNMARR314 pKa = 11.84TSAQQMGYY322 pKa = 9.7PNLLATRR329 pKa = 11.84IPHH332 pKa = 6.09APTWAGTSFRR342 pKa = 4.48

Molecular weight: 36.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

4197

342

3354

1399.0

156.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.1 ± 0.827

2.001 ± 0.634

6.338 ± 1.074

5.456 ± 0.777

4.265 ± 0.469

5.695 ± 1.243

2.43 ± 0.335

5.814 ± 0.312

5.575 ± 1.239

8.863 ± 1.442

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.249

4.694 ± 0.318

5.337 ± 0.142

3.812 ± 0.858

5.218 ± 1.469

8.649 ± 2.546

6.004 ± 0.842

6.838 ± 0.636

1.287 ± 0.197

3.264 ± 0.856
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski