Escherichia phage PA29

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Traversvirus; unclassified Traversvirus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7C1Z3|A0A0N7C1Z3_9CAUD WH2 domain-containing protein OS=Escherichia phage PA29 OX=1660369 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.36FHH4 pKa = 7.21EE5 pKa = 5.34SAICDD10 pKa = 3.52FRR12 pKa = 11.84ANANSVKK19 pKa = 9.25PQPIAVLFKK28 pKa = 10.94TMGAWAVLCFAADD41 pKa = 3.61DD42 pKa = 3.83TDD44 pKa = 3.42ARR46 pKa = 11.84MAIGQEE52 pKa = 4.02MEE54 pKa = 3.99MDD56 pKa = 3.55PTNDD60 pKa = 2.86EE61 pKa = 4.58FIIYY65 pKa = 7.71GAPSNYY71 pKa = 10.43LLDD74 pKa = 3.47TCNIYY79 pKa = 10.99NKK81 pKa = 10.37AAA83 pKa = 3.64

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7C1W2|A0A0N7C1W2_9CAUD Single stranded DNA-binding protein OS=Escherichia phage PA29 OX=1660369 PE=4 SV=1
MM1 pKa = 7.33QEE3 pKa = 4.03SKK5 pKa = 9.78TMKK8 pKa = 9.76NRR10 pKa = 11.84KK11 pKa = 9.04AKK13 pKa = 10.09ILLVRR18 pKa = 11.84RR19 pKa = 11.84NAPGVWQWVRR29 pKa = 11.84LSNRR33 pKa = 11.84RR34 pKa = 11.84MGLMKK39 pKa = 10.46YY40 pKa = 10.19YY41 pKa = 11.33GMMDD45 pKa = 3.45CGFCKK50 pKa = 10.38KK51 pKa = 10.1PSAEE55 pKa = 3.79QNRR58 pKa = 11.84WKK60 pKa = 10.86NHH62 pKa = 4.27LRR64 pKa = 11.84TKK66 pKa = 11.09GEE68 pKa = 3.81

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

18513

37

2793

199.1

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.388 ± 0.341

1.205 ± 0.22

5.899 ± 0.186

6.855 ± 0.296

3.176 ± 0.168

7.325 ± 0.459

1.745 ± 0.128

5.148 ± 0.326

5.953 ± 0.218

7.697 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.138

4.316 ± 0.164

4.354 ± 0.263

4.759 ± 0.304

6.768 ± 0.292

6.093 ± 0.234

5.731 ± 0.244

6.304 ± 0.228

1.539 ± 0.13

2.933 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski