Amino acid dipepetide frequency for Streptomyces phage Moozy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.788AlaAla: 11.788 ± 1.815
0.218AlaCys: 0.218 ± 0.141
5.312AlaAsp: 5.312 ± 0.693
5.385AlaGlu: 5.385 ± 0.673
3.056AlaPhe: 3.056 ± 0.438
7.859AlaGly: 7.859 ± 1.52
1.383AlaHis: 1.383 ± 0.358
6.112AlaIle: 6.112 ± 1.026
7.422AlaLys: 7.422 ± 0.865
8.732AlaLeu: 8.732 ± 1.307
2.547AlaMet: 2.547 ± 0.438
3.493AlaAsn: 3.493 ± 0.47
2.838AlaPro: 2.838 ± 0.45
2.765AlaGln: 2.765 ± 0.434
4.875AlaArg: 4.875 ± 0.503
4.73AlaSer: 4.73 ± 0.529
6.258AlaThr: 6.258 ± 0.786
7.349AlaVal: 7.349 ± 0.796
1.455AlaTrp: 1.455 ± 0.399
3.347AlaTyr: 3.347 ± 0.42
0.0AlaXaa: 0.0 ± 0.0
Cys
0.218CysAla: 0.218 ± 0.161
0.0CysCys: 0.0 ± 0.0
0.291CysAsp: 0.291 ± 0.136
0.509CysGlu: 0.509 ± 0.183
0.291CysPhe: 0.291 ± 0.132
0.218CysGly: 0.218 ± 0.134
0.0CysHis: 0.0 ± 0.0
0.437CysIle: 0.437 ± 0.226
0.073CysLys: 0.073 ± 0.074
0.509CysLeu: 0.509 ± 0.226
0.146CysMet: 0.146 ± 0.091
0.291CysAsn: 0.291 ± 0.169
0.218CysPro: 0.218 ± 0.119
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
0.364CysSer: 0.364 ± 0.158
0.146CysThr: 0.146 ± 0.128
0.218CysVal: 0.218 ± 0.143
0.073CysTrp: 0.073 ± 0.066
0.146CysTyr: 0.146 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
6.33AspAla: 6.33 ± 0.592
0.218AspCys: 0.218 ± 0.124
4.293AspAsp: 4.293 ± 0.742
5.967AspGlu: 5.967 ± 0.789
3.42AspPhe: 3.42 ± 0.53
6.112AspGly: 6.112 ± 0.75
0.655AspHis: 0.655 ± 0.182
3.274AspIle: 3.274 ± 0.655
2.474AspLys: 2.474 ± 0.352
4.948AspLeu: 4.948 ± 0.567
1.674AspMet: 1.674 ± 0.316
2.474AspAsn: 2.474 ± 0.366
3.711AspPro: 3.711 ± 0.667
2.328AspGln: 2.328 ± 0.353
2.838AspArg: 2.838 ± 0.523
2.765AspSer: 2.765 ± 0.394
3.565AspThr: 3.565 ± 0.472
3.929AspVal: 3.929 ± 0.537
0.946AspTrp: 0.946 ± 0.271
1.601AspTyr: 1.601 ± 0.311
0.0AspXaa: 0.0 ± 0.0
Glu
6.403GluAla: 6.403 ± 0.71
0.364GluCys: 0.364 ± 0.152
3.493GluAsp: 3.493 ± 0.636
5.385GluGlu: 5.385 ± 0.739
2.62GluPhe: 2.62 ± 0.458
3.929GluGly: 3.929 ± 0.594
1.019GluHis: 1.019 ± 0.266
4.366GluIle: 4.366 ± 0.489
4.148GluLys: 4.148 ± 0.626
5.239GluLeu: 5.239 ± 0.719
1.674GluMet: 1.674 ± 0.26
1.965GluAsn: 1.965 ± 0.472
2.692GluPro: 2.692 ± 0.442
3.129GluGln: 3.129 ± 0.506
3.638GluArg: 3.638 ± 0.577
2.765GluSer: 2.765 ± 0.467
4.148GluThr: 4.148 ± 0.578
4.802GluVal: 4.802 ± 0.805
1.31GluTrp: 1.31 ± 0.325
2.183GluTyr: 2.183 ± 0.379
0.0GluXaa: 0.0 ± 0.0
Phe
3.056PheAla: 3.056 ± 0.39
0.291PheCys: 0.291 ± 0.157
3.42PheAsp: 3.42 ± 0.535
2.11PheGlu: 2.11 ± 0.475
1.601PhePhe: 1.601 ± 0.297
3.274PheGly: 3.274 ± 0.507
0.364PheHis: 0.364 ± 0.148
1.746PheIle: 1.746 ± 0.319
2.474PheLys: 2.474 ± 0.665
2.838PheLeu: 2.838 ± 0.466
1.091PheMet: 1.091 ± 0.284
1.746PheAsn: 1.746 ± 0.339
0.946PhePro: 0.946 ± 0.209
0.873PheGln: 0.873 ± 0.233
2.765PheArg: 2.765 ± 0.42
1.892PheSer: 1.892 ± 0.433
2.692PheThr: 2.692 ± 0.51
2.328PheVal: 2.328 ± 0.381
0.509PheTrp: 0.509 ± 0.27
1.31PheTyr: 1.31 ± 0.364
0.0PheXaa: 0.0 ± 0.0
Gly
7.64GlyAla: 7.64 ± 1.013
0.218GlyCys: 0.218 ± 0.129
5.094GlyAsp: 5.094 ± 0.541
4.657GlyGlu: 4.657 ± 0.584
3.056GlyPhe: 3.056 ± 0.401
5.894GlyGly: 5.894 ± 0.864
1.019GlyHis: 1.019 ± 0.266
4.875GlyIle: 4.875 ± 1.037
4.802GlyLys: 4.802 ± 0.891
6.258GlyLeu: 6.258 ± 1.479
1.746GlyMet: 1.746 ± 0.338
1.819GlyAsn: 1.819 ± 0.355
2.765GlyPro: 2.765 ± 0.605
1.528GlyGln: 1.528 ± 0.353
3.202GlyArg: 3.202 ± 0.408
4.948GlySer: 4.948 ± 0.908
6.33GlyThr: 6.33 ± 0.729
6.476GlyVal: 6.476 ± 0.66
1.746GlyTrp: 1.746 ± 0.343
2.983GlyTyr: 2.983 ± 0.592
0.0GlyXaa: 0.0 ± 0.0
His
0.873HisAla: 0.873 ± 0.356
0.073HisCys: 0.073 ± 0.088
0.8HisAsp: 0.8 ± 0.262
1.237HisGlu: 1.237 ± 0.288
0.582HisPhe: 0.582 ± 0.174
0.873HisGly: 0.873 ± 0.207
0.509HisHis: 0.509 ± 0.181
0.946HisIle: 0.946 ± 0.249
0.655HisLys: 0.655 ± 0.201
1.164HisLeu: 1.164 ± 0.292
0.728HisMet: 0.728 ± 0.163
1.091HisAsn: 1.091 ± 0.356
0.655HisPro: 0.655 ± 0.242
0.873HisGln: 0.873 ± 0.247
0.509HisArg: 0.509 ± 0.155
1.383HisSer: 1.383 ± 0.361
0.728HisThr: 0.728 ± 0.234
0.946HisVal: 0.946 ± 0.232
0.218HisTrp: 0.218 ± 0.162
0.946HisTyr: 0.946 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
5.094IleAla: 5.094 ± 0.688
0.218IleCys: 0.218 ± 0.117
4.657IleAsp: 4.657 ± 0.522
4.22IleGlu: 4.22 ± 0.642
1.746IlePhe: 1.746 ± 0.364
4.22IleGly: 4.22 ± 1.066
0.946IleHis: 0.946 ± 0.312
1.965IleIle: 1.965 ± 0.381
3.42IleLys: 3.42 ± 0.644
4.439IleLeu: 4.439 ± 0.74
1.019IleMet: 1.019 ± 0.231
1.965IleAsn: 1.965 ± 0.272
2.183IlePro: 2.183 ± 0.39
1.819IleGln: 1.819 ± 0.357
2.547IleArg: 2.547 ± 0.522
2.838IleSer: 2.838 ± 0.412
4.657IleThr: 4.657 ± 0.581
3.565IleVal: 3.565 ± 0.378
0.582IleTrp: 0.582 ± 0.228
1.601IleTyr: 1.601 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
6.549LysAla: 6.549 ± 0.642
0.073LysCys: 0.073 ± 0.062
3.202LysAsp: 3.202 ± 0.499
3.493LysGlu: 3.493 ± 0.634
1.746LysPhe: 1.746 ± 0.541
4.075LysGly: 4.075 ± 1.014
1.237LysHis: 1.237 ± 0.418
3.493LysIle: 3.493 ± 0.606
4.511LysLys: 4.511 ± 0.645
5.967LysLeu: 5.967 ± 0.912
1.528LysMet: 1.528 ± 0.456
2.401LysAsn: 2.401 ± 0.438
2.547LysPro: 2.547 ± 0.403
2.401LysGln: 2.401 ± 0.369
2.911LysArg: 2.911 ± 0.493
4.075LysSer: 4.075 ± 0.7
4.002LysThr: 4.002 ± 0.582
4.657LysVal: 4.657 ± 0.589
0.946LysTrp: 0.946 ± 0.288
2.183LysTyr: 2.183 ± 0.398
0.0LysXaa: 0.0 ± 0.0
Leu
9.387LeuAla: 9.387 ± 1.324
0.291LeuCys: 0.291 ± 0.143
5.967LeuAsp: 5.967 ± 0.606
4.002LeuGlu: 4.002 ± 0.665
2.765LeuPhe: 2.765 ± 0.462
6.258LeuGly: 6.258 ± 0.935
1.091LeuHis: 1.091 ± 0.294
3.638LeuIle: 3.638 ± 0.851
6.039LeuLys: 6.039 ± 0.978
4.875LeuLeu: 4.875 ± 0.671
1.674LeuMet: 1.674 ± 0.298
3.42LeuAsn: 3.42 ± 0.504
3.129LeuPro: 3.129 ± 0.419
2.62LeuGln: 2.62 ± 0.522
4.511LeuArg: 4.511 ± 0.607
5.967LeuSer: 5.967 ± 0.916
5.239LeuThr: 5.239 ± 0.726
6.185LeuVal: 6.185 ± 0.655
0.8LeuTrp: 0.8 ± 0.249
2.765LeuTyr: 2.765 ± 0.451
0.0LeuXaa: 0.0 ± 0.0
Met
1.746MetAla: 1.746 ± 0.505
0.218MetCys: 0.218 ± 0.13
1.674MetAsp: 1.674 ± 0.343
2.183MetGlu: 2.183 ± 0.464
0.8MetPhe: 0.8 ± 0.256
1.528MetGly: 1.528 ± 0.331
0.364MetHis: 0.364 ± 0.134
1.601MetIle: 1.601 ± 0.323
1.237MetLys: 1.237 ± 0.398
2.547MetLeu: 2.547 ± 0.431
0.655MetMet: 0.655 ± 0.256
1.383MetAsn: 1.383 ± 0.398
1.31MetPro: 1.31 ± 0.306
0.582MetGln: 0.582 ± 0.169
0.873MetArg: 0.873 ± 0.268
2.11MetSer: 2.11 ± 0.389
1.455MetThr: 1.455 ± 0.297
1.892MetVal: 1.892 ± 0.358
0.146MetTrp: 0.146 ± 0.101
0.873MetTyr: 0.873 ± 0.254
0.0MetXaa: 0.0 ± 0.0
Asn
3.929AsnAla: 3.929 ± 0.569
0.146AsnCys: 0.146 ± 0.102
1.746AsnAsp: 1.746 ± 0.336
2.183AsnGlu: 2.183 ± 0.464
1.383AsnPhe: 1.383 ± 0.338
3.274AsnGly: 3.274 ± 0.497
0.873AsnHis: 0.873 ± 0.253
1.674AsnIle: 1.674 ± 0.257
2.256AsnLys: 2.256 ± 0.365
2.547AsnLeu: 2.547 ± 0.436
0.655AsnMet: 0.655 ± 0.179
1.528AsnAsn: 1.528 ± 0.261
2.547AsnPro: 2.547 ± 0.512
1.383AsnGln: 1.383 ± 0.323
3.129AsnArg: 3.129 ± 0.599
2.62AsnSer: 2.62 ± 0.473
2.256AsnThr: 2.256 ± 0.313
2.765AsnVal: 2.765 ± 0.483
0.8AsnTrp: 0.8 ± 0.275
1.164AsnTyr: 1.164 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
4.075ProAla: 4.075 ± 0.608
0.364ProCys: 0.364 ± 0.182
3.42ProAsp: 3.42 ± 0.756
3.056ProGlu: 3.056 ± 0.524
1.601ProPhe: 1.601 ± 0.277
3.129ProGly: 3.129 ± 0.396
0.364ProHis: 0.364 ± 0.176
2.11ProIle: 2.11 ± 0.461
2.692ProLys: 2.692 ± 0.33
2.911ProLeu: 2.911 ± 0.53
1.237ProMet: 1.237 ± 0.294
1.746ProAsn: 1.746 ± 0.353
1.892ProPro: 1.892 ± 0.431
1.019ProGln: 1.019 ± 0.247
1.746ProArg: 1.746 ± 0.456
2.547ProSer: 2.547 ± 0.534
3.857ProThr: 3.857 ± 0.467
2.983ProVal: 2.983 ± 0.534
0.509ProTrp: 0.509 ± 0.213
1.819ProTyr: 1.819 ± 0.482
0.0ProXaa: 0.0 ± 0.0
Gln
3.129GlnAla: 3.129 ± 0.532
0.218GlnCys: 0.218 ± 0.123
1.091GlnAsp: 1.091 ± 0.249
1.601GlnGlu: 1.601 ± 0.379
1.164GlnPhe: 1.164 ± 0.258
2.692GlnGly: 2.692 ± 0.409
1.091GlnHis: 1.091 ± 0.288
2.183GlnIle: 2.183 ± 0.399
1.237GlnLys: 1.237 ± 0.261
3.056GlnLeu: 3.056 ± 0.606
1.237GlnMet: 1.237 ± 0.217
1.019GlnAsn: 1.019 ± 0.249
1.601GlnPro: 1.601 ± 0.411
1.237GlnGln: 1.237 ± 0.229
1.965GlnArg: 1.965 ± 0.365
1.892GlnSer: 1.892 ± 0.429
2.11GlnThr: 2.11 ± 0.464
2.037GlnVal: 2.037 ± 0.378
0.582GlnTrp: 0.582 ± 0.205
1.455GlnTyr: 1.455 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
4.293ArgAla: 4.293 ± 0.854
0.291ArgCys: 0.291 ± 0.16
2.911ArgAsp: 2.911 ± 0.478
3.202ArgGlu: 3.202 ± 0.453
1.746ArgPhe: 1.746 ± 0.401
2.474ArgGly: 2.474 ± 0.488
0.946ArgHis: 0.946 ± 0.268
2.037ArgIle: 2.037 ± 0.358
3.493ArgLys: 3.493 ± 0.386
4.657ArgLeu: 4.657 ± 0.743
1.819ArgMet: 1.819 ± 0.363
2.401ArgAsn: 2.401 ± 0.356
2.328ArgPro: 2.328 ± 0.391
2.62ArgGln: 2.62 ± 0.65
3.42ArgArg: 3.42 ± 0.765
2.62ArgSer: 2.62 ± 0.549
3.565ArgThr: 3.565 ± 0.645
3.857ArgVal: 3.857 ± 0.654
0.582ArgTrp: 0.582 ± 0.246
1.091ArgTyr: 1.091 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
5.021SerAla: 5.021 ± 0.772
0.073SerCys: 0.073 ± 0.088
3.711SerAsp: 3.711 ± 0.578
3.202SerGlu: 3.202 ± 0.487
1.455SerPhe: 1.455 ± 0.283
5.967SerGly: 5.967 ± 0.905
0.946SerHis: 0.946 ± 0.299
2.474SerIle: 2.474 ± 0.319
3.638SerLys: 3.638 ± 0.509
5.166SerLeu: 5.166 ± 0.694
1.091SerMet: 1.091 ± 0.247
2.328SerAsn: 2.328 ± 0.412
2.765SerPro: 2.765 ± 0.422
2.037SerGln: 2.037 ± 0.393
2.401SerArg: 2.401 ± 0.432
3.638SerSer: 3.638 ± 0.757
5.166SerThr: 5.166 ± 0.886
4.802SerVal: 4.802 ± 0.621
1.31SerTrp: 1.31 ± 0.354
1.528SerTyr: 1.528 ± 0.336
0.0SerXaa: 0.0 ± 0.0
Thr
5.894ThrAla: 5.894 ± 0.82
0.291ThrCys: 0.291 ± 0.157
4.584ThrAsp: 4.584 ± 0.627
4.366ThrGlu: 4.366 ± 0.685
2.692ThrPhe: 2.692 ± 0.562
6.185ThrGly: 6.185 ± 0.792
0.946ThrHis: 0.946 ± 0.36
4.002ThrIle: 4.002 ± 0.58
4.075ThrLys: 4.075 ± 0.472
5.748ThrLeu: 5.748 ± 0.769
1.019ThrMet: 1.019 ± 0.321
2.11ThrAsn: 2.11 ± 0.366
4.22ThrPro: 4.22 ± 0.628
2.11ThrGln: 2.11 ± 0.449
3.129ThrArg: 3.129 ± 0.56
4.075ThrSer: 4.075 ± 0.809
6.33ThrThr: 6.33 ± 1.057
5.53ThrVal: 5.53 ± 0.729
1.091ThrTrp: 1.091 ± 0.255
3.274ThrTyr: 3.274 ± 0.537
0.0ThrXaa: 0.0 ± 0.0
Val
7.495ValAla: 7.495 ± 0.662
0.291ValCys: 0.291 ± 0.149
4.657ValAsp: 4.657 ± 0.575
5.53ValGlu: 5.53 ± 0.708
2.692ValPhe: 2.692 ± 0.439
5.457ValGly: 5.457 ± 0.804
1.019ValHis: 1.019 ± 0.398
4.439ValIle: 4.439 ± 0.652
3.784ValLys: 3.784 ± 0.399
6.185ValLeu: 6.185 ± 0.699
2.11ValMet: 2.11 ± 0.487
2.692ValAsn: 2.692 ± 0.452
3.056ValPro: 3.056 ± 0.396
1.819ValGln: 1.819 ± 0.465
4.002ValArg: 4.002 ± 0.72
4.002ValSer: 4.002 ± 0.409
5.53ValThr: 5.53 ± 0.73
4.73ValVal: 4.73 ± 0.502
1.383ValTrp: 1.383 ± 0.304
2.547ValTyr: 2.547 ± 0.518
0.0ValXaa: 0.0 ± 0.0
Trp
1.019TrpAla: 1.019 ± 0.259
0.073TrpCys: 0.073 ± 0.066
0.873TrpAsp: 0.873 ± 0.329
1.091TrpGlu: 1.091 ± 0.333
0.8TrpPhe: 0.8 ± 0.222
1.383TrpGly: 1.383 ± 0.413
0.291TrpHis: 0.291 ± 0.141
0.728TrpIle: 0.728 ± 0.293
0.873TrpLys: 0.873 ± 0.261
0.946TrpLeu: 0.946 ± 0.341
0.509TrpMet: 0.509 ± 0.201
1.164TrpAsn: 1.164 ± 0.355
0.437TrpPro: 0.437 ± 0.145
0.364TrpGln: 0.364 ± 0.138
0.437TrpArg: 0.437 ± 0.195
1.674TrpSer: 1.674 ± 0.46
1.237TrpThr: 1.237 ± 0.424
1.164TrpVal: 1.164 ± 0.386
0.218TrpTrp: 0.218 ± 0.15
0.291TrpTyr: 0.291 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.056TyrAla: 3.056 ± 0.917
0.218TyrCys: 0.218 ± 0.147
2.328TyrAsp: 2.328 ± 0.481
1.892TyrGlu: 1.892 ± 0.527
2.11TyrPhe: 2.11 ± 0.417
2.256TyrGly: 2.256 ± 0.468
0.728TyrHis: 0.728 ± 0.246
1.601TyrIle: 1.601 ± 0.353
2.692TyrLys: 2.692 ± 0.425
1.892TyrLeu: 1.892 ± 0.344
0.946TyrMet: 0.946 ± 0.259
1.819TyrAsn: 1.819 ± 0.277
1.237TyrPro: 1.237 ± 0.347
1.091TyrGln: 1.091 ± 0.26
1.455TyrArg: 1.455 ± 0.303
1.819TyrSer: 1.819 ± 0.321
2.401TyrThr: 2.401 ± 0.418
3.202TyrVal: 3.202 ± 0.435
0.364TyrTrp: 0.364 ± 0.189
1.091TyrTyr: 1.091 ± 0.391
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13744 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski