Streptococcus satellite phage Javan631
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZSN9|A0A4D5ZSN9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan631 OX=2558795 GN=JavanS631_0007 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.4 NLEE5 pKa = 4.17 EE6 pKa = 5.44 KK7 pKa = 10.32 IEE9 pKa = 4.01 PVVIILDD16 pKa = 3.82 EE17 pKa = 4.39 EE18 pKa = 4.86 DD19 pKa = 5.34 DD20 pKa = 4.57 FDD22 pKa = 5.98 FEE24 pKa = 4.44 PTDD27 pKa = 4.67 EE28 pKa = 4.34 EE29 pKa = 5.21 LQDD32 pKa = 3.54 QYY34 pKa = 11.6 EE35 pKa = 4.32 AEE37 pKa = 4.3 CLEE40 pKa = 5.78 DD41 pKa = 3.71 DD42 pKa = 4.11 TQDD45 pKa = 3.43 VINWINEE52 pKa = 4.11 SNTLAEE58 pKa = 4.29 YY59 pKa = 11.21 VSMEE63 pKa = 4.26 FEE65 pKa = 4.68 NMNKK69 pKa = 10.07 DD70 pKa = 3.67 DD71 pKa = 5.63 LGTSLEE77 pKa = 4.21 NASQCLDD84 pKa = 4.31 AIVGMLEE91 pKa = 3.77 NGTLILAGGKK101 pKa = 9.32 HH102 pKa = 5.43 EE103 pKa = 4.13 NN104 pKa = 3.61
Molecular weight: 11.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.135
IPC2_protein 3.516
IPC_protein 3.465
Toseland 3.274
ProMoST 3.63
Dawson 3.452
Bjellqvist 3.605
Wikipedia 3.363
Rodwell 3.3
Grimsley 3.198
Solomon 3.427
Lehninger 3.376
Nozaki 3.579
DTASelect 3.719
Thurlkill 3.325
EMBOSS 3.376
Sillero 3.579
Patrickios 2.727
IPC_peptide 3.427
IPC2_peptide 3.554
IPC2.peptide.svr19 3.669
Protein with the highest isoelectric point:
>tr|A0A4D5ZTC6|A0A4D5ZTC6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan631 OX=2558795 GN=JavanS631_0004 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.18 YY6 pKa = 10.59 KK7 pKa = 9.86 KK8 pKa = 10.3 KK9 pKa = 10.54 NGTIVYY15 pKa = 9.63 RR16 pKa = 11.84 SQVYY20 pKa = 10.49 LGVDD24 pKa = 4.02 VITGKK29 pKa = 8.3 EE30 pKa = 3.87 VKK32 pKa = 9.42 TRR34 pKa = 11.84 ITARR38 pKa = 11.84 TKK40 pKa = 10.91 KK41 pKa = 9.72 EE42 pKa = 3.87 LKK44 pKa = 10.53 LLAKK48 pKa = 10.07 QKK50 pKa = 10.28 QNEE53 pKa = 4.58 FIRR56 pKa = 11.84 NGSTVHH62 pKa = 7.16 SEE64 pKa = 4.1 IKK66 pKa = 9.79 IKK68 pKa = 10.42 SYY70 pKa = 11.4 KK71 pKa = 10.18 EE72 pKa = 3.81 LTDD75 pKa = 3.74 LWWNSYY81 pKa = 10.64 KK82 pKa = 9.94 NTVKK86 pKa = 10.5 PNTVGSVSLLLKK98 pKa = 10.27 NHH100 pKa = 7.51 IIPTFGDD107 pKa = 3.54 YY108 pKa = 10.98 KK109 pKa = 10.46 LDD111 pKa = 4.51 KK112 pKa = 9.87 ITTPLIQNKK121 pKa = 6.88 VNKK124 pKa = 9.08 WADD127 pKa = 3.24 KK128 pKa = 11.0 ANNNEE133 pKa = 4.02 SGAFAHH139 pKa = 6.33 YY140 pKa = 10.33 DD141 pKa = 3.32 KK142 pKa = 11.42 LHH144 pKa = 6.45 ALNKK148 pKa = 10.21 RR149 pKa = 11.84 ILQYY153 pKa = 11.04 GVTMQLLDD161 pKa = 4.05 NNPALNVVLPRR172 pKa = 11.84 KK173 pKa = 9.6 KK174 pKa = 10.18 KK175 pKa = 10.15 RR176 pKa = 11.84 EE177 pKa = 4.04 KK178 pKa = 10.17 TSLKK182 pKa = 10.63 YY183 pKa = 10.52 FDD185 pKa = 3.4 NTEE188 pKa = 3.94 LKK190 pKa = 10.74 KK191 pKa = 10.89 FLDD194 pKa = 3.89 YY195 pKa = 11.39 LDD197 pKa = 4.99 GLNQSNYY204 pKa = 7.31 RR205 pKa = 11.84 HH206 pKa = 6.04 HH207 pKa = 7.45 FEE209 pKa = 3.71 VTLYY213 pKa = 10.92 KK214 pKa = 10.49 FLLATGCRR222 pKa = 11.84 INEE225 pKa = 4.0 ALALSWSDD233 pKa = 2.88 IDD235 pKa = 5.27 LEE237 pKa = 4.38 SSSLSITKK245 pKa = 8.8 TLNRR249 pKa = 11.84 YY250 pKa = 9.35 VEE252 pKa = 4.31 VNTPKK257 pKa = 10.3 SQASIRR263 pKa = 11.84 TIDD266 pKa = 3.35 IDD268 pKa = 3.57 KK269 pKa = 9.93 ATVLMLKK276 pKa = 9.72 QYY278 pKa = 10.89 RR279 pKa = 11.84 NRR281 pKa = 11.84 QRR283 pKa = 11.84 IQGLEE288 pKa = 3.66 IGLVPDD294 pKa = 3.87 IVFSDD299 pKa = 4.82 FINKK303 pKa = 8.57 YY304 pKa = 11.04 VNDD307 pKa = 3.4 QTLYY311 pKa = 9.64 TRR313 pKa = 11.84 LRR315 pKa = 11.84 THH317 pKa = 7.03 FKK319 pKa = 10.33 RR320 pKa = 11.84 AGVSNIGFHH329 pKa = 6.37 GFRR332 pKa = 11.84 HH333 pKa = 4.66 THH335 pKa = 5.52 ATLLYY340 pKa = 10.37 NAGIDD345 pKa = 3.78 PKK347 pKa = 10.95 ALQHH351 pKa = 6.52 RR352 pKa = 11.84 LGHH355 pKa = 5.34 STISMTLDD363 pKa = 3.4 TYY365 pKa = 11.55 SHH367 pKa = 7.1 LSKK370 pKa = 10.96 EE371 pKa = 4.3 NAKK374 pKa = 10.34 KK375 pKa = 10.33 AVSFFEE381 pKa = 4.35 TAVSSLL387 pKa = 3.45
Molecular weight: 44.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.045
IPC2_protein 9.282
IPC_protein 9.18
Toseland 9.999
ProMoST 9.633
Dawson 10.189
Bjellqvist 9.823
Wikipedia 10.335
Rodwell 10.745
Grimsley 10.248
Solomon 10.204
Lehninger 10.175
Nozaki 9.97
DTASelect 9.823
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.087
Patrickios 10.306
IPC_peptide 10.204
IPC2_peptide 8.258
IPC2.peptide.svr19 8.261
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
3518
48
672
195.4
22.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.827 ± 0.886
0.597 ± 0.213
6.083 ± 0.628
7.504 ± 0.864
4.434 ± 0.395
5.372 ± 1.057
1.45 ± 0.299
7.675 ± 0.323
9.608 ± 0.899
10.432 ± 0.476
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.757 ± 0.341
6.737 ± 0.361
2.16 ± 0.196
3.894 ± 0.406
3.61 ± 0.53
6.652 ± 0.703
5.259 ± 0.443
5.429 ± 0.242
0.711 ± 0.104
3.809 ± 0.53
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here