Oenococcus kitaharae DSM 17330
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1818 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9WFS3|G9WFS3_9LACO Isopentenyl-diphosphate delta-isomerase OS=Oenococcus kitaharae DSM 17330 OX=1045004 GN=fni PE=3 SV=1
MM1 pKa = 8.04 DD2 pKa = 5.45 FEE4 pKa = 5.6 DD5 pKa = 4.68 LQALTDD11 pKa = 4.2 LDD13 pKa = 4.03 EE14 pKa = 6.09 ADD16 pKa = 4.46 LVVALNHH23 pKa = 5.4 LHH25 pKa = 7.62 DD26 pKa = 5.63 RR27 pKa = 11.84 GLLFNGYY34 pKa = 8.87 IDD36 pKa = 4.27 EE37 pKa = 4.4 EE38 pKa = 4.37 TGEE41 pKa = 4.43 VVTDD45 pKa = 4.46 DD46 pKa = 3.61 IGYY49 pKa = 10.04 LYY51 pKa = 11.1 KK52 pKa = 10.79 LANN55 pKa = 3.63
Molecular weight: 6.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 1.863
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|G9WJR9|G9WJR9_9LACO Peptide chain release factor 3 OS=Oenococcus kitaharae DSM 17330 OX=1045004 GN=prfC PE=3 SV=1
MM1 pKa = 7.88 PKK3 pKa = 9.48 MKK5 pKa = 10.02 TSRR8 pKa = 11.84 ASAKK12 pKa = 9.94 RR13 pKa = 11.84 FKK15 pKa = 9.66 KK16 pKa = 8.39 TASGGFKK23 pKa = 10.21 SGQAFTSHH31 pKa = 6.83 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.41 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 GTAMMNRR52 pKa = 11.84 INVKK56 pKa = 9.78 RR57 pKa = 11.84 YY58 pKa = 10.15 AKK60 pKa = 10.44 VLSNLL65 pKa = 3.51
Molecular weight: 7.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.427
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.149
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.956
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1818
0
1818
551158
50
1583
303.2
33.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.737 ± 0.062
0.33 ± 0.011
5.967 ± 0.06
4.786 ± 0.058
4.878 ± 0.054
6.481 ± 0.047
2.108 ± 0.027
7.359 ± 0.051
6.24 ± 0.056
10.279 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.414 ± 0.026
4.675 ± 0.037
3.579 ± 0.032
4.886 ± 0.048
4.245 ± 0.041
6.621 ± 0.044
5.448 ± 0.039
6.5 ± 0.043
1.058 ± 0.023
3.407 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here