Amino acid dipepetide frequency for butyrate-producing bacterium SM4/1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.862AlaAla: 9.862 ± 0.252
1.38AlaCys: 1.38 ± 0.068
4.834AlaAsp: 4.834 ± 0.119
6.592AlaGlu: 6.592 ± 0.161
3.434AlaPhe: 3.434 ± 0.089
8.085AlaGly: 8.085 ± 0.199
1.07AlaHis: 1.07 ± 0.057
4.434AlaIle: 4.434 ± 0.136
4.166AlaLys: 4.166 ± 0.111
7.682AlaLeu: 7.682 ± 0.166
2.451AlaMet: 2.451 ± 0.091
2.085AlaAsn: 2.085 ± 0.083
2.279AlaPro: 2.279 ± 0.083
2.504AlaGln: 2.504 ± 0.102
3.837AlaArg: 3.837 ± 0.115
4.4AlaSer: 4.4 ± 0.122
2.735AlaThr: 2.735 ± 0.102
7.448AlaVal: 7.448 ± 0.143
0.696AlaTrp: 0.696 ± 0.042
2.547AlaTyr: 2.547 ± 0.085
0.0AlaXaa: 0.0 ± 0.0
Cys
1.149CysAla: 1.149 ± 0.055
0.394CysCys: 0.394 ± 0.036
0.769CysAsp: 0.769 ± 0.046
0.98CysGlu: 0.98 ± 0.053
0.701CysPhe: 0.701 ± 0.043
1.659CysGly: 1.659 ± 0.073
0.386CysHis: 0.386 ± 0.041
1.147CysIle: 1.147 ± 0.054
0.544CysLys: 0.544 ± 0.039
1.473CysLeu: 1.473 ± 0.071
0.524CysMet: 0.524 ± 0.04
0.403CysAsn: 0.403 ± 0.034
0.825CysPro: 0.825 ± 0.049
0.524CysGln: 0.524 ± 0.039
1.279CysArg: 1.279 ± 0.063
1.144CysSer: 1.144 ± 0.056
0.755CysThr: 0.755 ± 0.043
1.039CysVal: 1.039 ± 0.046
0.127CysTrp: 0.127 ± 0.02
0.521CysTyr: 0.521 ± 0.038
0.0CysXaa: 0.0 ± 0.0
Asp
3.8AspAla: 3.8 ± 0.105
1.003AspCys: 1.003 ± 0.055
2.332AspAsp: 2.332 ± 0.087
4.203AspGlu: 4.203 ± 0.13
2.423AspPhe: 2.423 ± 0.085
4.687AspGly: 4.687 ± 0.14
0.732AspHis: 0.732 ± 0.044
3.558AspIle: 3.558 ± 0.102
2.639AspLys: 2.639 ± 0.093
4.062AspLeu: 4.062 ± 0.118
1.89AspMet: 1.89 ± 0.079
1.631AspAsn: 1.631 ± 0.072
1.969AspPro: 1.969 ± 0.075
1.33AspGln: 1.33 ± 0.067
3.386AspArg: 3.386 ± 0.106
3.152AspSer: 3.152 ± 0.102
2.699AspThr: 2.699 ± 0.098
3.13AspVal: 3.13 ± 0.106
0.656AspTrp: 0.656 ± 0.048
2.518AspTyr: 2.518 ± 0.106
0.0AspXaa: 0.0 ± 0.0
Glu
6.654GluAla: 6.654 ± 0.166
0.749GluCys: 0.749 ± 0.039
3.882GluAsp: 3.882 ± 0.107
8.409GluGlu: 8.409 ± 0.186
2.569GluPhe: 2.569 ± 0.09
5.254GluGly: 5.254 ± 0.139
1.369GluHis: 1.369 ± 0.063
5.335GluIle: 5.335 ± 0.11
6.718GluLys: 6.718 ± 0.166
7.211GluLeu: 7.211 ± 0.136
2.445GluMet: 2.445 ± 0.078
3.73GluAsn: 3.73 ± 0.118
2.344GluPro: 2.344 ± 0.086
3.237GluGln: 3.237 ± 0.105
4.71GluArg: 4.71 ± 0.147
3.749GluSer: 3.749 ± 0.106
4.062GluThr: 4.062 ± 0.135
3.935GluVal: 3.935 ± 0.12
0.696GluTrp: 0.696 ± 0.045
2.707GluTyr: 2.707 ± 0.088
0.0GluXaa: 0.0 ± 0.0
Phe
3.02PheAla: 3.02 ± 0.087
0.887PheCys: 0.887 ± 0.05
2.245PheAsp: 2.245 ± 0.076
2.592PheGlu: 2.592 ± 0.079
1.907PhePhe: 1.907 ± 0.084
3.369PheGly: 3.369 ± 0.112
0.862PheHis: 0.862 ± 0.044
2.668PheIle: 2.668 ± 0.089
1.741PheLys: 1.741 ± 0.067
4.454PheLeu: 4.454 ± 0.149
1.223PheMet: 1.223 ± 0.057
1.265PheAsn: 1.265 ± 0.069
1.617PhePro: 1.617 ± 0.065
1.42PheGln: 1.42 ± 0.055
2.265PheArg: 2.265 ± 0.074
3.223PheSer: 3.223 ± 0.102
2.172PheThr: 2.172 ± 0.088
2.594PheVal: 2.594 ± 0.08
0.47PheTrp: 0.47 ± 0.036
1.628PheTyr: 1.628 ± 0.071
0.0PheXaa: 0.0 ± 0.0
Gly
5.64GlyAla: 5.64 ± 0.137
1.487GlyCys: 1.487 ± 0.063
3.487GlyAsp: 3.487 ± 0.102
5.69GlyGlu: 5.69 ± 0.131
3.304GlyPhe: 3.304 ± 0.106
5.696GlyGly: 5.696 ± 0.148
1.197GlyHis: 1.197 ± 0.065
6.209GlyIle: 6.209 ± 0.159
5.521GlyLys: 5.521 ± 0.132
6.859GlyLeu: 6.859 ± 0.144
2.727GlyMet: 2.727 ± 0.094
2.935GlyAsn: 2.935 ± 0.097
1.741GlyPro: 1.741 ± 0.063
2.561GlyGln: 2.561 ± 0.072
4.662GlyArg: 4.662 ± 0.121
4.84GlySer: 4.84 ± 0.129
4.547GlyThr: 4.547 ± 0.116
4.992GlyVal: 4.992 ± 0.128
0.896GlyTrp: 0.896 ± 0.066
2.935GlyTyr: 2.935 ± 0.088
0.0GlyXaa: 0.0 ± 0.0
His
1.073HisAla: 1.073 ± 0.054
0.423HisCys: 0.423 ± 0.034
0.803HisAsp: 0.803 ± 0.039
1.014HisGlu: 1.014 ± 0.061
0.854HisPhe: 0.854 ± 0.051
1.256HisGly: 1.256 ± 0.068
0.366HisHis: 0.366 ± 0.032
1.177HisIle: 1.177 ± 0.066
0.676HisLys: 0.676 ± 0.041
1.608HisLeu: 1.608 ± 0.071
0.445HisMet: 0.445 ± 0.032
0.535HisAsn: 0.535 ± 0.035
0.887HisPro: 0.887 ± 0.054
0.425HisGln: 0.425 ± 0.035
1.003HisArg: 1.003 ± 0.053
0.955HisSer: 0.955 ± 0.048
0.828HisThr: 0.828 ± 0.052
0.997HisVal: 0.997 ± 0.052
0.189HisTrp: 0.189 ± 0.021
0.704HisTyr: 0.704 ± 0.051
0.0HisXaa: 0.0 ± 0.0
Ile
5.344IleAla: 5.344 ± 0.136
1.327IleCys: 1.327 ± 0.065
3.389IleAsp: 3.389 ± 0.104
4.141IleGlu: 4.141 ± 0.11
2.69IlePhe: 2.69 ± 0.097
4.828IleGly: 4.828 ± 0.138
1.116IleHis: 1.116 ± 0.054
3.986IleIle: 3.986 ± 0.134
3.017IleLys: 3.017 ± 0.088
6.918IleLeu: 6.918 ± 0.168
1.687IleMet: 1.687 ± 0.066
2.144IleAsn: 2.144 ± 0.075
3.209IlePro: 3.209 ± 0.109
2.27IleGln: 2.27 ± 0.075
4.161IleArg: 4.161 ± 0.115
4.378IleSer: 4.378 ± 0.131
3.341IleThr: 3.341 ± 0.102
4.38IleVal: 4.38 ± 0.122
0.6IleTrp: 0.6 ± 0.046
2.296IleTyr: 2.296 ± 0.09
0.0IleXaa: 0.0 ± 0.0
Lys
4.918LysAla: 4.918 ± 0.13
0.642LysCys: 0.642 ± 0.04
3.147LysAsp: 3.147 ± 0.096
5.876LysGlu: 5.876 ± 0.139
1.639LysPhe: 1.639 ± 0.076
3.854LysGly: 3.854 ± 0.111
0.887LysHis: 0.887 ± 0.049
3.949LysIle: 3.949 ± 0.123
5.502LysLys: 5.502 ± 0.147
4.609LysLeu: 4.609 ± 0.117
1.924LysMet: 1.924 ± 0.082
2.955LysAsn: 2.955 ± 0.094
2.003LysPro: 2.003 ± 0.072
2.014LysGln: 2.014 ± 0.078
3.673LysArg: 3.673 ± 0.115
3.093LysSer: 3.093 ± 0.098
3.49LysThr: 3.49 ± 0.09
3.335LysVal: 3.335 ± 0.1
0.603LysTrp: 0.603 ± 0.044
2.144LysTyr: 2.144 ± 0.08
0.0LysXaa: 0.0 ± 0.0
Leu
7.772LeuAla: 7.772 ± 0.168
1.682LeuCys: 1.682 ± 0.065
4.828LeuAsp: 4.828 ± 0.103
6.285LeuGlu: 6.285 ± 0.152
4.321LeuPhe: 4.321 ± 0.135
6.234LeuGly: 6.234 ± 0.16
1.586LeuHis: 1.586 ± 0.069
5.704LeuIle: 5.704 ± 0.142
5.724LeuLys: 5.724 ± 0.125
9.369LeuLeu: 9.369 ± 0.236
2.811LeuMet: 2.811 ± 0.09
3.496LeuAsn: 3.496 ± 0.105
4.11LeuPro: 4.11 ± 0.125
2.547LeuGln: 2.547 ± 0.094
4.885LeuArg: 4.885 ± 0.144
6.868LeuSer: 6.868 ± 0.176
5.04LeuThr: 5.04 ± 0.117
5.4LeuVal: 5.4 ± 0.127
0.859LeuTrp: 0.859 ± 0.058
3.217LeuTyr: 3.217 ± 0.095
0.0LeuXaa: 0.0 ± 0.0
Met
2.828MetAla: 2.828 ± 0.081
0.389MetCys: 0.389 ± 0.034
1.887MetAsp: 1.887 ± 0.087
3.065MetGlu: 3.065 ± 0.098
0.98MetPhe: 0.98 ± 0.058
2.394MetGly: 2.394 ± 0.1
0.355MetHis: 0.355 ± 0.035
1.98MetIle: 1.98 ± 0.068
2.532MetLys: 2.532 ± 0.078
2.558MetLeu: 2.558 ± 0.087
0.961MetMet: 0.961 ± 0.06
1.428MetAsn: 1.428 ± 0.064
1.175MetPro: 1.175 ± 0.062
0.947MetGln: 0.947 ± 0.056
1.586MetArg: 1.586 ± 0.068
1.727MetSer: 1.727 ± 0.076
1.837MetThr: 1.837 ± 0.071
1.732MetVal: 1.732 ± 0.075
0.245MetTrp: 0.245 ± 0.026
0.811MetTyr: 0.811 ± 0.047
0.0MetXaa: 0.0 ± 0.0
Asn
2.685AsnAla: 2.685 ± 0.093
0.577AsnCys: 0.577 ± 0.042
1.603AsnAsp: 1.603 ± 0.063
2.251AsnGlu: 2.251 ± 0.078
1.468AsnPhe: 1.468 ± 0.074
3.121AsnGly: 3.121 ± 0.096
0.687AsnHis: 0.687 ± 0.051
2.62AsnIle: 2.62 ± 0.081
1.701AsnLys: 1.701 ± 0.078
3.262AsnLeu: 3.262 ± 0.096
1.2AsnMet: 1.2 ± 0.063
1.18AsnAsn: 1.18 ± 0.054
1.839AsnPro: 1.839 ± 0.068
1.316AsnGln: 1.316 ± 0.051
2.287AsnArg: 2.287 ± 0.089
1.961AsnSer: 1.961 ± 0.084
1.879AsnThr: 1.879 ± 0.067
2.251AsnVal: 2.251 ± 0.095
0.375AsnTrp: 0.375 ± 0.031
1.397AsnTyr: 1.397 ± 0.068
0.0AsnXaa: 0.0 ± 0.0
Pro
3.211ProAla: 3.211 ± 0.095
0.592ProCys: 0.592 ± 0.044
2.355ProAsp: 2.355 ± 0.093
3.713ProGlu: 3.713 ± 0.098
1.724ProPhe: 1.724 ± 0.071
3.217ProGly: 3.217 ± 0.106
0.634ProHis: 0.634 ± 0.042
1.949ProIle: 1.949 ± 0.079
1.718ProLys: 1.718 ± 0.075
3.206ProLeu: 3.206 ± 0.092
1.101ProMet: 1.101 ± 0.055
1.118ProAsn: 1.118 ± 0.062
1.034ProPro: 1.034 ± 0.05
1.144ProGln: 1.144 ± 0.053
1.31ProArg: 1.31 ± 0.06
2.223ProSer: 2.223 ± 0.078
1.392ProThr: 1.392 ± 0.061
3.248ProVal: 3.248 ± 0.093
0.349ProTrp: 0.349 ± 0.029
1.411ProTyr: 1.411 ± 0.063
0.0ProXaa: 0.0 ± 0.0
Gln
2.961GlnAla: 2.961 ± 0.111
0.366GlnCys: 0.366 ± 0.034
1.406GlnAsp: 1.406 ± 0.061
2.783GlnGlu: 2.783 ± 0.107
1.408GlnPhe: 1.408 ± 0.068
2.121GlnGly: 2.121 ± 0.079
0.377GlnHis: 0.377 ± 0.034
2.485GlnIle: 2.485 ± 0.087
2.569GlnLys: 2.569 ± 0.1
2.611GlnLeu: 2.611 ± 0.079
1.223GlnMet: 1.223 ± 0.059
1.4GlnAsn: 1.4 ± 0.075
1.017GlnPro: 1.017 ± 0.052
0.975GlnGln: 0.975 ± 0.066
1.71GlnArg: 1.71 ± 0.087
1.749GlnSer: 1.749 ± 0.077
1.856GlnThr: 1.856 ± 0.079
2.079GlnVal: 2.079 ± 0.069
0.386GlnTrp: 0.386 ± 0.036
1.208GlnTyr: 1.208 ± 0.055
0.0GlnXaa: 0.0 ± 0.0
Arg
3.98ArgAla: 3.98 ± 0.104
0.701ArgCys: 0.701 ± 0.05
2.716ArgAsp: 2.716 ± 0.086
6.144ArgGlu: 6.144 ± 0.186
2.178ArgPhe: 2.178 ± 0.08
3.648ArgGly: 3.648 ± 0.112
0.955ArgHis: 0.955 ± 0.054
3.727ArgIle: 3.727 ± 0.121
4.211ArgLys: 4.211 ± 0.137
5.454ArgLeu: 5.454 ± 0.146
2.09ArgMet: 2.09 ± 0.079
2.172ArgAsn: 2.172 ± 0.074
1.927ArgPro: 1.927 ± 0.084
2.397ArgGln: 2.397 ± 0.09
4.194ArgArg: 4.194 ± 0.143
2.806ArgSer: 2.806 ± 0.091
2.594ArgThr: 2.594 ± 0.088
3.062ArgVal: 3.062 ± 0.096
0.552ArgTrp: 0.552 ± 0.04
2.0ArgTyr: 2.0 ± 0.083
0.0ArgXaa: 0.0 ± 0.0
Ser
4.735SerAla: 4.735 ± 0.126
1.087SerCys: 1.087 ± 0.061
2.899SerAsp: 2.899 ± 0.086
4.144SerGlu: 4.144 ± 0.131
2.918SerPhe: 2.918 ± 0.096
5.82SerGly: 5.82 ± 0.156
1.087SerHis: 1.087 ± 0.049
3.572SerIle: 3.572 ± 0.111
2.431SerLys: 2.431 ± 0.087
5.82SerLeu: 5.82 ± 0.142
1.921SerMet: 1.921 ± 0.078
1.586SerAsn: 1.586 ± 0.065
2.299SerPro: 2.299 ± 0.081
2.107SerGln: 2.107 ± 0.084
3.825SerArg: 3.825 ± 0.108
3.668SerSer: 3.668 ± 0.121
2.547SerThr: 2.547 ± 0.095
4.423SerVal: 4.423 ± 0.117
0.738SerTrp: 0.738 ± 0.044
2.186SerTyr: 2.186 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
5.271ThrAla: 5.271 ± 0.133
0.648ThrCys: 0.648 ± 0.043
2.972ThrAsp: 2.972 ± 0.103
4.161ThrGlu: 4.161 ± 0.118
1.958ThrPhe: 1.958 ± 0.071
4.935ThrGly: 4.935 ± 0.134
0.701ThrHis: 0.701 ± 0.045
3.045ThrIle: 3.045 ± 0.092
2.361ThrLys: 2.361 ± 0.095
4.352ThrLeu: 4.352 ± 0.108
1.29ThrMet: 1.29 ± 0.059
1.431ThrAsn: 1.431 ± 0.075
2.121ThrPro: 2.121 ± 0.076
1.332ThrGln: 1.332 ± 0.073
2.175ThrArg: 2.175 ± 0.083
2.51ThrSer: 2.51 ± 0.094
2.228ThrThr: 2.228 ± 0.089
4.648ThrVal: 4.648 ± 0.124
0.406ThrTrp: 0.406 ± 0.034
1.589ThrTyr: 1.589 ± 0.078
0.0ThrXaa: 0.0 ± 0.0
Val
4.53ValAla: 4.53 ± 0.129
1.197ValCys: 1.197 ± 0.059
3.456ValAsp: 3.456 ± 0.112
4.13ValGlu: 4.13 ± 0.119
3.13ValPhe: 3.13 ± 0.112
4.172ValGly: 4.172 ± 0.132
0.98ValHis: 0.98 ± 0.052
4.868ValIle: 4.868 ± 0.134
4.172ValLys: 4.172 ± 0.108
6.496ValLeu: 6.496 ± 0.144
2.11ValMet: 2.11 ± 0.077
2.316ValAsn: 2.316 ± 0.085
2.651ValPro: 2.651 ± 0.084
1.98ValGln: 1.98 ± 0.084
3.58ValArg: 3.58 ± 0.102
4.687ValSer: 4.687 ± 0.123
3.817ValThr: 3.817 ± 0.12
4.186ValVal: 4.186 ± 0.123
0.668ValTrp: 0.668 ± 0.048
2.366ValTyr: 2.366 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.572TrpAla: 0.572 ± 0.042
0.158TrpCys: 0.158 ± 0.022
0.656TrpAsp: 0.656 ± 0.049
0.772TrpGlu: 0.772 ± 0.05
0.414TrpPhe: 0.414 ± 0.039
0.738TrpGly: 0.738 ± 0.047
0.18TrpHis: 0.18 ± 0.022
0.608TrpIle: 0.608 ± 0.045
0.761TrpLys: 0.761 ± 0.049
1.045TrpLeu: 1.045 ± 0.055
0.372TrpMet: 0.372 ± 0.03
0.476TrpAsn: 0.476 ± 0.034
0.327TrpPro: 0.327 ± 0.031
0.423TrpGln: 0.423 ± 0.037
0.58TrpArg: 0.58 ± 0.051
0.42TrpSer: 0.42 ± 0.034
0.394TrpThr: 0.394 ± 0.034
0.501TrpVal: 0.501 ± 0.04
0.138TrpTrp: 0.138 ± 0.018
0.451TrpTyr: 0.451 ± 0.045
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.516TyrAla: 2.516 ± 0.078
0.654TyrCys: 0.654 ± 0.049
2.135TyrAsp: 2.135 ± 0.087
2.823TyrGlu: 2.823 ± 0.082
1.6TyrPhe: 1.6 ± 0.073
2.837TyrGly: 2.837 ± 0.098
0.69TyrHis: 0.69 ± 0.043
2.2TyrIle: 2.2 ± 0.076
1.552TyrLys: 1.552 ± 0.068
3.69TyrLeu: 3.69 ± 0.103
1.02TyrMet: 1.02 ± 0.056
1.375TyrAsn: 1.375 ± 0.065
1.276TyrPro: 1.276 ± 0.062
1.239TyrGln: 1.239 ± 0.06
2.4TyrArg: 2.4 ± 0.088
2.262TyrSer: 2.262 ± 0.082
1.921TyrThr: 1.921 ± 0.063
2.11TyrVal: 2.11 ± 0.07
0.338TyrTrp: 0.338 ± 0.034
1.6TyrTyr: 1.6 ± 0.08
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1684 proteins (354993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski