Hubei tombus-like virus 42

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KG95|A0A1L3KG95_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei tombus-like virus 42 OX=1923290 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 5.74ASLFPLNIWIHH13 pKa = 6.3SSQQHH18 pKa = 4.18MNIRR22 pKa = 11.84RR23 pKa = 11.84AISHH27 pKa = 5.93TLVCVVQGCAVVKK40 pKa = 10.56DD41 pKa = 3.73RR42 pKa = 11.84LIAHH46 pKa = 6.23ITVNIDD52 pKa = 3.02DD53 pKa = 4.67PMPVVLQISPPSKK66 pKa = 9.03LQMAINHH73 pKa = 6.19ACNLRR78 pKa = 11.84SIVLSSTICVAKK90 pKa = 10.08RR91 pKa = 11.84LSGAISQAAQSIHH104 pKa = 6.25TGLHH108 pKa = 5.71LALHH112 pKa = 5.21YY113 pKa = 9.18TLLGAAVTAIIIWFIKK129 pKa = 8.16TRR131 pKa = 11.84KK132 pKa = 9.07CRR134 pKa = 11.84IIKK137 pKa = 9.22VRR139 pKa = 11.84NHH141 pKa = 5.44NQMADD146 pKa = 2.63IRR148 pKa = 11.84RR149 pKa = 11.84IKK151 pKa = 10.14EE152 pKa = 3.61QFATKK157 pKa = 10.01IKK159 pKa = 10.58RR160 pKa = 11.84LTPPEE165 pKa = 4.08FEE167 pKa = 4.11QGKK170 pKa = 8.91HH171 pKa = 5.81VICAWEE177 pKa = 4.08RR178 pKa = 11.84KK179 pKa = 5.45TTEE182 pKa = 3.74QIINEE187 pKa = 4.18WFHH190 pKa = 6.51DD191 pKa = 3.59QGIKK195 pKa = 10.46FRR197 pKa = 11.84DD198 pKa = 3.24IGGSRR203 pKa = 11.84VRR205 pKa = 11.84GSNYY209 pKa = 8.04ATSRR213 pKa = 11.84HH214 pKa = 5.4ICNPTLDD221 pKa = 3.61SSDD224 pKa = 2.75ILRR227 pKa = 11.84RR228 pKa = 11.84AKK230 pKa = 10.31QPTTIFSEE238 pKa = 5.01CTCLGQHH245 pKa = 6.32CPYY248 pKa = 10.29KK249 pKa = 10.66NEE251 pKa = 3.95YY252 pKa = 8.23PGAMFIHH259 pKa = 6.85SDD261 pKa = 3.89YY262 pKa = 11.47YY263 pKa = 11.5LDD265 pKa = 5.0ANALTDD271 pKa = 3.83CVRR274 pKa = 11.84SHH276 pKa = 6.11TFVVTHH282 pKa = 7.13RR283 pKa = 11.84FTGDD287 pKa = 2.45EE288 pKa = 3.98GTFFNEE294 pKa = 4.22AQWVKK299 pKa = 10.46HH300 pKa = 4.41GTKK303 pKa = 8.59ITMTTPDD310 pKa = 3.34GTKK313 pKa = 10.47YY314 pKa = 9.94NHH316 pKa = 7.59PYY318 pKa = 10.19NLWEE322 pKa = 4.15NEE324 pKa = 3.75GCIVGSSNACIYY336 pKa = 10.93AKK338 pKa = 10.5VGSTEE343 pKa = 3.86TSDD346 pKa = 3.2IYY348 pKa = 11.63YY349 pKa = 10.01LFPADD354 pKa = 3.56GTYY357 pKa = 10.95LKK359 pKa = 10.51DD360 pKa = 4.19DD361 pKa = 3.71RR362 pKa = 11.84LALKK366 pKa = 10.44RR367 pKa = 11.84STEE370 pKa = 3.99LAEE373 pKa = 4.27PLHH376 pKa = 6.07PSGYY380 pKa = 10.7VIIKK384 pKa = 9.63SDD386 pKa = 3.27QFFIYY391 pKa = 9.96DD392 pKa = 3.51DD393 pKa = 4.66HH394 pKa = 7.57DD395 pKa = 3.75NLMIKK400 pKa = 10.51GPVDD404 pKa = 3.67IISRR408 pKa = 11.84MKK410 pKa = 10.73LRR412 pKa = 11.84LGLAARR418 pKa = 11.84TSNFNSVFFSYY429 pKa = 10.55LQARR433 pKa = 11.84CQAEE437 pKa = 4.53EE438 pKa = 4.08IGITDD443 pKa = 4.4PLRR446 pKa = 11.84LTEE449 pKa = 3.9FALRR453 pKa = 11.84EE454 pKa = 4.17TEE456 pKa = 4.61QFALTIAPRR465 pKa = 11.84WSRR468 pKa = 11.84QWDD471 pKa = 3.73PANMTLCTGLKK482 pKa = 10.05LRR484 pKa = 11.84LHH486 pKa = 6.94RR487 pKa = 11.84MYY489 pKa = 10.39NTIIRR494 pKa = 11.84ATFDD498 pKa = 3.74LLPTCRR504 pKa = 11.84LRR506 pKa = 11.84CIFAHH511 pKa = 7.29RR512 pKa = 11.84ILAPWAYY519 pKa = 10.47RR520 pKa = 11.84EE521 pKa = 4.1ITLPGYY527 pKa = 10.31IMEE530 pKa = 4.65VRR532 pKa = 11.84PQHH535 pKa = 5.47TDD537 pKa = 2.73VRR539 pKa = 11.84GLRR542 pKa = 11.84PSGQPFPDD550 pKa = 4.97DD551 pKa = 3.6GTDD554 pKa = 3.68NDD556 pKa = 4.52AEE558 pKa = 4.48PDD560 pKa = 3.3NSEE563 pKa = 4.18SVRR566 pKa = 11.84ADD568 pKa = 3.41SVSGEE573 pKa = 4.22FDD575 pKa = 3.35SQSGNEE581 pKa = 3.83SDD583 pKa = 4.83GYY585 pKa = 8.99QTATSSRR592 pKa = 11.84SHH594 pKa = 5.87VRR596 pKa = 11.84NEE598 pKa = 3.67APIVDD603 pKa = 4.17TDD605 pKa = 3.65ASTTSTPGQSGRR617 pKa = 11.84TASLLPTDD625 pKa = 4.71GMDD628 pKa = 4.01SEE630 pKa = 4.97TSRR633 pKa = 11.84QIRR636 pKa = 11.84PITRR640 pKa = 11.84QDD642 pKa = 3.15SADD645 pKa = 3.65FEE647 pKa = 4.44KK648 pKa = 11.16SLIIFSGPSSSTSLPPTVVLDD669 pKa = 3.91PNRR672 pKa = 11.84NTVSSALYY680 pKa = 9.92DD681 pKa = 3.97DD682 pKa = 6.06PILLTAEE689 pKa = 4.38QFRR692 pKa = 11.84SLCQSKK698 pKa = 10.71DD699 pKa = 3.45AVLKK703 pKa = 10.84AHH705 pKa = 7.12FSTKK709 pKa = 7.85RR710 pKa = 11.84TRR712 pKa = 11.84EE713 pKa = 3.93QIYY716 pKa = 9.25HH717 pKa = 5.56QLVQEE722 pKa = 3.86ARR724 pKa = 11.84RR725 pKa = 11.84AKK727 pKa = 9.83RR728 pKa = 11.84HH729 pKa = 5.32PSEE732 pKa = 4.06TAGGGISEE740 pKa = 4.54DD741 pKa = 3.6PGNGVVPTPSRR752 pKa = 11.84TTTVRR757 pKa = 11.84PVGQEE762 pKa = 3.65IPKK765 pKa = 8.92MAPQTTRR772 pKa = 11.84DD773 pKa = 3.64SQNEE777 pKa = 4.24SGQSRR782 pKa = 11.84DD783 pKa = 3.27NVQRR787 pKa = 11.84RR788 pKa = 11.84PSSEE792 pKa = 3.6LHH794 pKa = 6.36KK795 pKa = 11.15ARR797 pKa = 11.84DD798 pKa = 3.8HH799 pKa = 6.15PQVHH803 pKa = 6.27RR804 pKa = 11.84PKK806 pKa = 10.27KK807 pKa = 8.4HH808 pKa = 5.49QSTHH812 pKa = 5.24RR813 pKa = 11.84RR814 pKa = 11.84VSGHH818 pKa = 5.66SRR820 pKa = 11.84SLHH823 pKa = 4.19QRR825 pKa = 11.84HH826 pKa = 5.94RR827 pKa = 11.84EE828 pKa = 3.94TRR830 pKa = 11.84SSRR833 pKa = 11.84PIPGQRR839 pKa = 11.84HH840 pKa = 4.36GAKK843 pKa = 10.15DD844 pKa = 3.3ARR846 pKa = 11.84KK847 pKa = 9.62EE848 pKa = 4.02DD849 pKa = 3.71GG850 pKa = 3.66

Molecular weight:
95.58 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KG95|A0A1L3KG95_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei tombus-like virus 42 OX=1923290 PE=4 SV=1
MM1 pKa = 7.21FVDD4 pKa = 4.7CGRR7 pKa = 11.84VDD9 pKa = 3.5SPFRR13 pKa = 11.84TTARR17 pKa = 11.84IMMPNLTTANLSVPIVCQEE36 pKa = 3.7NLIASLEE43 pKa = 4.18TRR45 pKa = 11.84VMGTKK50 pKa = 9.37PQPHH54 pKa = 7.35PGLTFAMKK62 pKa = 9.49PQLLIPMPLLPQHH75 pKa = 6.9PANQDD80 pKa = 2.87GLLAYY85 pKa = 7.94CQRR88 pKa = 11.84MGWTAKK94 pKa = 10.6LVDD97 pKa = 4.87KK98 pKa = 10.98YY99 pKa = 11.43DD100 pKa = 3.87PSQDD104 pKa = 3.54KK105 pKa = 10.41IVQILRR111 pKa = 11.84NPSSYY116 pKa = 11.1SADD119 pKa = 3.38PALAPLCHH127 pKa = 6.08QRR129 pKa = 11.84SYY131 pKa = 8.08WTPTEE136 pKa = 3.91IPLALHH142 pKa = 5.62YY143 pKa = 7.36TTTLYY148 pKa = 10.83SSQPSSLDD156 pKa = 2.94HH157 pKa = 6.13CVNRR161 pKa = 11.84KK162 pKa = 6.86TLSSKK167 pKa = 9.43LTSPPNGLEE176 pKa = 3.76NKK178 pKa = 8.64FTINSSKK185 pKa = 10.74KK186 pKa = 8.98HH187 pKa = 4.85VEE189 pKa = 3.89PRR191 pKa = 11.84DD192 pKa = 3.51TPLRR196 pKa = 11.84LLVEE200 pKa = 4.62EE201 pKa = 4.38FLKK204 pKa = 10.03TPEE207 pKa = 4.32MEE209 pKa = 4.29WFPHH213 pKa = 5.97HH214 pKa = 7.15LEE216 pKa = 4.63PLPFDD221 pKa = 3.46QWVKK225 pKa = 10.68RR226 pKa = 11.84YY227 pKa = 8.37PKK229 pKa = 9.34WRR231 pKa = 11.84RR232 pKa = 11.84KK233 pKa = 7.55QLEE236 pKa = 3.94IAKK239 pKa = 9.32MKK241 pKa = 9.67VDD243 pKa = 3.45KK244 pKa = 11.42VGITSKK250 pKa = 10.67DD251 pKa = 2.99ARR253 pKa = 11.84VRR255 pKa = 11.84NFIKK259 pKa = 10.65RR260 pKa = 11.84EE261 pKa = 4.11TTHH264 pKa = 7.13KK265 pKa = 10.12FTDD268 pKa = 3.55PRR270 pKa = 11.84NISPRR275 pKa = 11.84TDD277 pKa = 2.96EE278 pKa = 4.29FLVTVGPYY286 pKa = 9.37ISAIEE291 pKa = 4.09KK292 pKa = 9.31HH293 pKa = 5.64AHH295 pKa = 5.69HH296 pKa = 7.04APFLVKK302 pKa = 10.55GMGLKK307 pKa = 9.04MRR309 pKa = 11.84AKK311 pKa = 10.72KK312 pKa = 9.34MDD314 pKa = 3.46KK315 pKa = 10.43LLGFGRR321 pKa = 11.84YY322 pKa = 9.43LEE324 pKa = 4.2VDD326 pKa = 4.43FTRR329 pKa = 11.84FDD331 pKa = 3.46KK332 pKa = 11.04TISADD337 pKa = 3.1IIRR340 pKa = 11.84IVEE343 pKa = 4.27KK344 pKa = 11.07YY345 pKa = 10.69LLTKK349 pKa = 10.05PYY351 pKa = 10.74SRR353 pKa = 11.84DD354 pKa = 2.52HH355 pKa = 6.41HH356 pKa = 7.1AYY358 pKa = 8.18HH359 pKa = 6.29QCMKK363 pKa = 10.65QLTTTSGVSYY373 pKa = 10.45FGTRR377 pKa = 11.84YY378 pKa = 9.08KK379 pKa = 11.1VKK381 pKa = 9.35GTRR384 pKa = 11.84CSGDD388 pKa = 2.7AHH390 pKa = 6.14TSLGNGLINRR400 pKa = 11.84FIIWLCLRR408 pKa = 11.84KK409 pKa = 9.8LPQNAWDD416 pKa = 4.09SMHH419 pKa = 6.9EE420 pKa = 4.16GDD422 pKa = 5.56DD423 pKa = 4.41GIIGVSLSYY432 pKa = 11.23VNQAVYY438 pKa = 10.86NLQFLACLGFEE449 pKa = 4.64SKK451 pKa = 10.96LKK453 pKa = 8.54VCKK456 pKa = 10.46SIEE459 pKa = 3.96EE460 pKa = 4.2VVFCGRR466 pKa = 11.84RR467 pKa = 11.84HH468 pKa = 4.7VRR470 pKa = 11.84TAKK473 pKa = 10.34EE474 pKa = 3.76SATICDD480 pKa = 4.12VIRR483 pKa = 11.84TMRR486 pKa = 11.84KK487 pKa = 9.22FNTTCSLGPPDD498 pKa = 4.42LLLYY502 pKa = 10.88AKK504 pKa = 10.12ALSYY508 pKa = 11.18NYY510 pKa = 9.6TDD512 pKa = 4.21ADD514 pKa = 3.95TPIIGPVSYY523 pKa = 10.96AVARR527 pKa = 11.84CLAHH531 pKa = 7.1CNIKK535 pKa = 10.52YY536 pKa = 9.98SRR538 pKa = 11.84KK539 pKa = 7.68QFKK542 pKa = 10.09RR543 pKa = 11.84ALKK546 pKa = 8.84TAVLEE551 pKa = 4.12RR552 pKa = 11.84WVLNDD557 pKa = 3.48NIGSISYY564 pKa = 9.96HH565 pKa = 6.22RR566 pKa = 11.84LLNAKK571 pKa = 9.46PPDD574 pKa = 3.4ISPYY578 pKa = 10.54LITASLMLNRR588 pKa = 11.84PTSVQKK594 pKa = 10.97LMQQQ598 pKa = 3.14

Molecular weight:
68.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1448

598

850

724.0

81.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.423 ± 0.345

2.279 ± 0.139

5.525 ± 0.409

4.213 ± 0.324

3.384 ± 0.077

4.972 ± 0.379

3.867 ± 0.317

6.423 ± 0.548

5.525 ± 1.213

8.425 ± 1.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.425

3.729 ± 0.071

6.215 ± 0.388

4.282 ± 0.467

7.528 ± 0.711

8.011 ± 0.7

7.666 ± 0.288

5.18 ± 0.307

1.174 ± 0.099

3.039 ± 0.489

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski