Hubei tombus-like virus 42
Average proteome isoelectric point is 8.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KG95|A0A1L3KG95_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei tombus-like virus 42 OX=1923290 PE=4 SV=1
MM1 pKa = 7.83 DD2 pKa = 5.74 ASLFPLNIWIHH13 pKa = 6.3 SSQQHH18 pKa = 4.18 MNIRR22 pKa = 11.84 RR23 pKa = 11.84 AISHH27 pKa = 5.93 TLVCVVQGCAVVKK40 pKa = 10.56 DD41 pKa = 3.73 RR42 pKa = 11.84 LIAHH46 pKa = 6.23 ITVNIDD52 pKa = 3.02 DD53 pKa = 4.67 PMPVVLQISPPSKK66 pKa = 9.03 LQMAINHH73 pKa = 6.19 ACNLRR78 pKa = 11.84 SIVLSSTICVAKK90 pKa = 10.08 RR91 pKa = 11.84 LSGAISQAAQSIHH104 pKa = 6.25 TGLHH108 pKa = 5.71 LALHH112 pKa = 5.21 YY113 pKa = 9.18 TLLGAAVTAIIIWFIKK129 pKa = 8.16 TRR131 pKa = 11.84 KK132 pKa = 9.07 CRR134 pKa = 11.84 IIKK137 pKa = 9.22 VRR139 pKa = 11.84 NHH141 pKa = 5.44 NQMADD146 pKa = 2.63 IRR148 pKa = 11.84 RR149 pKa = 11.84 IKK151 pKa = 10.14 EE152 pKa = 3.61 QFATKK157 pKa = 10.01 IKK159 pKa = 10.58 RR160 pKa = 11.84 LTPPEE165 pKa = 4.08 FEE167 pKa = 4.11 QGKK170 pKa = 8.91 HH171 pKa = 5.81 VICAWEE177 pKa = 4.08 RR178 pKa = 11.84 KK179 pKa = 5.45 TTEE182 pKa = 3.74 QIINEE187 pKa = 4.18 WFHH190 pKa = 6.51 DD191 pKa = 3.59 QGIKK195 pKa = 10.46 FRR197 pKa = 11.84 DD198 pKa = 3.24 IGGSRR203 pKa = 11.84 VRR205 pKa = 11.84 GSNYY209 pKa = 8.04 ATSRR213 pKa = 11.84 HH214 pKa = 5.4 ICNPTLDD221 pKa = 3.61 SSDD224 pKa = 2.75 ILRR227 pKa = 11.84 RR228 pKa = 11.84 AKK230 pKa = 10.31 QPTTIFSEE238 pKa = 5.01 CTCLGQHH245 pKa = 6.32 CPYY248 pKa = 10.29 KK249 pKa = 10.66 NEE251 pKa = 3.95 YY252 pKa = 8.23 PGAMFIHH259 pKa = 6.85 SDD261 pKa = 3.89 YY262 pKa = 11.47 YY263 pKa = 11.5 LDD265 pKa = 5.0 ANALTDD271 pKa = 3.83 CVRR274 pKa = 11.84 SHH276 pKa = 6.11 TFVVTHH282 pKa = 7.13 RR283 pKa = 11.84 FTGDD287 pKa = 2.45 EE288 pKa = 3.98 GTFFNEE294 pKa = 4.22 AQWVKK299 pKa = 10.46 HH300 pKa = 4.41 GTKK303 pKa = 8.59 ITMTTPDD310 pKa = 3.34 GTKK313 pKa = 10.47 YY314 pKa = 9.94 NHH316 pKa = 7.59 PYY318 pKa = 10.19 NLWEE322 pKa = 4.15 NEE324 pKa = 3.75 GCIVGSSNACIYY336 pKa = 10.93 AKK338 pKa = 10.5 VGSTEE343 pKa = 3.86 TSDD346 pKa = 3.2 IYY348 pKa = 11.63 YY349 pKa = 10.01 LFPADD354 pKa = 3.56 GTYY357 pKa = 10.95 LKK359 pKa = 10.51 DD360 pKa = 4.19 DD361 pKa = 3.71 RR362 pKa = 11.84 LALKK366 pKa = 10.44 RR367 pKa = 11.84 STEE370 pKa = 3.99 LAEE373 pKa = 4.27 PLHH376 pKa = 6.07 PSGYY380 pKa = 10.7 VIIKK384 pKa = 9.63 SDD386 pKa = 3.27 QFFIYY391 pKa = 9.96 DD392 pKa = 3.51 DD393 pKa = 4.66 HH394 pKa = 7.57 DD395 pKa = 3.75 NLMIKK400 pKa = 10.51 GPVDD404 pKa = 3.67 IISRR408 pKa = 11.84 MKK410 pKa = 10.73 LRR412 pKa = 11.84 LGLAARR418 pKa = 11.84 TSNFNSVFFSYY429 pKa = 10.55 LQARR433 pKa = 11.84 CQAEE437 pKa = 4.53 EE438 pKa = 4.08 IGITDD443 pKa = 4.4 PLRR446 pKa = 11.84 LTEE449 pKa = 3.9 FALRR453 pKa = 11.84 EE454 pKa = 4.17 TEE456 pKa = 4.61 QFALTIAPRR465 pKa = 11.84 WSRR468 pKa = 11.84 QWDD471 pKa = 3.73 PANMTLCTGLKK482 pKa = 10.05 LRR484 pKa = 11.84 LHH486 pKa = 6.94 RR487 pKa = 11.84 MYY489 pKa = 10.39 NTIIRR494 pKa = 11.84 ATFDD498 pKa = 3.74 LLPTCRR504 pKa = 11.84 LRR506 pKa = 11.84 CIFAHH511 pKa = 7.29 RR512 pKa = 11.84 ILAPWAYY519 pKa = 10.47 RR520 pKa = 11.84 EE521 pKa = 4.1 ITLPGYY527 pKa = 10.31 IMEE530 pKa = 4.65 VRR532 pKa = 11.84 PQHH535 pKa = 5.47 TDD537 pKa = 2.73 VRR539 pKa = 11.84 GLRR542 pKa = 11.84 PSGQPFPDD550 pKa = 4.97 DD551 pKa = 3.6 GTDD554 pKa = 3.68 NDD556 pKa = 4.52 AEE558 pKa = 4.48 PDD560 pKa = 3.3 NSEE563 pKa = 4.18 SVRR566 pKa = 11.84 ADD568 pKa = 3.41 SVSGEE573 pKa = 4.22 FDD575 pKa = 3.35 SQSGNEE581 pKa = 3.83 SDD583 pKa = 4.83 GYY585 pKa = 8.99 QTATSSRR592 pKa = 11.84 SHH594 pKa = 5.87 VRR596 pKa = 11.84 NEE598 pKa = 3.67 APIVDD603 pKa = 4.17 TDD605 pKa = 3.65 ASTTSTPGQSGRR617 pKa = 11.84 TASLLPTDD625 pKa = 4.71 GMDD628 pKa = 4.01 SEE630 pKa = 4.97 TSRR633 pKa = 11.84 QIRR636 pKa = 11.84 PITRR640 pKa = 11.84 QDD642 pKa = 3.15 SADD645 pKa = 3.65 FEE647 pKa = 4.44 KK648 pKa = 11.16 SLIIFSGPSSSTSLPPTVVLDD669 pKa = 3.91 PNRR672 pKa = 11.84 NTVSSALYY680 pKa = 9.92 DD681 pKa = 3.97 DD682 pKa = 6.06 PILLTAEE689 pKa = 4.38 QFRR692 pKa = 11.84 SLCQSKK698 pKa = 10.71 DD699 pKa = 3.45 AVLKK703 pKa = 10.84 AHH705 pKa = 7.12 FSTKK709 pKa = 7.85 RR710 pKa = 11.84 TRR712 pKa = 11.84 EE713 pKa = 3.93 QIYY716 pKa = 9.25 HH717 pKa = 5.56 QLVQEE722 pKa = 3.86 ARR724 pKa = 11.84 RR725 pKa = 11.84 AKK727 pKa = 9.83 RR728 pKa = 11.84 HH729 pKa = 5.32 PSEE732 pKa = 4.06 TAGGGISEE740 pKa = 4.54 DD741 pKa = 3.6 PGNGVVPTPSRR752 pKa = 11.84 TTTVRR757 pKa = 11.84 PVGQEE762 pKa = 3.65 IPKK765 pKa = 8.92 MAPQTTRR772 pKa = 11.84 DD773 pKa = 3.64 SQNEE777 pKa = 4.24 SGQSRR782 pKa = 11.84 DD783 pKa = 3.27 NVQRR787 pKa = 11.84 RR788 pKa = 11.84 PSSEE792 pKa = 3.6 LHH794 pKa = 6.36 KK795 pKa = 11.15 ARR797 pKa = 11.84 DD798 pKa = 3.8 HH799 pKa = 6.15 PQVHH803 pKa = 6.27 RR804 pKa = 11.84 PKK806 pKa = 10.27 KK807 pKa = 8.4 HH808 pKa = 5.49 QSTHH812 pKa = 5.24 RR813 pKa = 11.84 RR814 pKa = 11.84 VSGHH818 pKa = 5.66 SRR820 pKa = 11.84 SLHH823 pKa = 4.19 QRR825 pKa = 11.84 HH826 pKa = 5.94 RR827 pKa = 11.84 EE828 pKa = 3.94 TRR830 pKa = 11.84 SSRR833 pKa = 11.84 PIPGQRR839 pKa = 11.84 HH840 pKa = 4.36 GAKK843 pKa = 10.15 DD844 pKa = 3.3 ARR846 pKa = 11.84 KK847 pKa = 9.62 EE848 pKa = 4.02 DD849 pKa = 3.71 GG850 pKa = 3.66
Molecular weight: 95.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.071
IPC2_protein 8.17
IPC_protein 7.995
Toseland 7.878
ProMoST 8.58
Dawson 8.785
Bjellqvist 9.033
Wikipedia 8.77
Rodwell 8.843
Grimsley 7.936
Solomon 8.843
Lehninger 8.843
Nozaki 9.238
DTASelect 8.799
Thurlkill 8.873
EMBOSS 8.975
Sillero 9.151
Patrickios 4.673
IPC_peptide 8.843
IPC2_peptide 8.009
IPC2.peptide.svr19 7.991
Protein with the highest isoelectric point:
>tr|A0A1L3KG95|A0A1L3KG95_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei tombus-like virus 42 OX=1923290 PE=4 SV=1
MM1 pKa = 7.21 FVDD4 pKa = 4.7 CGRR7 pKa = 11.84 VDD9 pKa = 3.5 SPFRR13 pKa = 11.84 TTARR17 pKa = 11.84 IMMPNLTTANLSVPIVCQEE36 pKa = 3.7 NLIASLEE43 pKa = 4.18 TRR45 pKa = 11.84 VMGTKK50 pKa = 9.37 PQPHH54 pKa = 7.35 PGLTFAMKK62 pKa = 9.49 PQLLIPMPLLPQHH75 pKa = 6.9 PANQDD80 pKa = 2.87 GLLAYY85 pKa = 7.94 CQRR88 pKa = 11.84 MGWTAKK94 pKa = 10.6 LVDD97 pKa = 4.87 KK98 pKa = 10.98 YY99 pKa = 11.43 DD100 pKa = 3.87 PSQDD104 pKa = 3.54 KK105 pKa = 10.41 IVQILRR111 pKa = 11.84 NPSSYY116 pKa = 11.1 SADD119 pKa = 3.38 PALAPLCHH127 pKa = 6.08 QRR129 pKa = 11.84 SYY131 pKa = 8.08 WTPTEE136 pKa = 3.91 IPLALHH142 pKa = 5.62 YY143 pKa = 7.36 TTTLYY148 pKa = 10.83 SSQPSSLDD156 pKa = 2.94 HH157 pKa = 6.13 CVNRR161 pKa = 11.84 KK162 pKa = 6.86 TLSSKK167 pKa = 9.43 LTSPPNGLEE176 pKa = 3.76 NKK178 pKa = 8.64 FTINSSKK185 pKa = 10.74 KK186 pKa = 8.98 HH187 pKa = 4.85 VEE189 pKa = 3.89 PRR191 pKa = 11.84 DD192 pKa = 3.51 TPLRR196 pKa = 11.84 LLVEE200 pKa = 4.62 EE201 pKa = 4.38 FLKK204 pKa = 10.03 TPEE207 pKa = 4.32 MEE209 pKa = 4.29 WFPHH213 pKa = 5.97 HH214 pKa = 7.15 LEE216 pKa = 4.63 PLPFDD221 pKa = 3.46 QWVKK225 pKa = 10.68 RR226 pKa = 11.84 YY227 pKa = 8.37 PKK229 pKa = 9.34 WRR231 pKa = 11.84 RR232 pKa = 11.84 KK233 pKa = 7.55 QLEE236 pKa = 3.94 IAKK239 pKa = 9.32 MKK241 pKa = 9.67 VDD243 pKa = 3.45 KK244 pKa = 11.42 VGITSKK250 pKa = 10.67 DD251 pKa = 2.99 ARR253 pKa = 11.84 VRR255 pKa = 11.84 NFIKK259 pKa = 10.65 RR260 pKa = 11.84 EE261 pKa = 4.11 TTHH264 pKa = 7.13 KK265 pKa = 10.12 FTDD268 pKa = 3.55 PRR270 pKa = 11.84 NISPRR275 pKa = 11.84 TDD277 pKa = 2.96 EE278 pKa = 4.29 FLVTVGPYY286 pKa = 9.37 ISAIEE291 pKa = 4.09 KK292 pKa = 9.31 HH293 pKa = 5.64 AHH295 pKa = 5.69 HH296 pKa = 7.04 APFLVKK302 pKa = 10.55 GMGLKK307 pKa = 9.04 MRR309 pKa = 11.84 AKK311 pKa = 10.72 KK312 pKa = 9.34 MDD314 pKa = 3.46 KK315 pKa = 10.43 LLGFGRR321 pKa = 11.84 YY322 pKa = 9.43 LEE324 pKa = 4.2 VDD326 pKa = 4.43 FTRR329 pKa = 11.84 FDD331 pKa = 3.46 KK332 pKa = 11.04 TISADD337 pKa = 3.1 IIRR340 pKa = 11.84 IVEE343 pKa = 4.27 KK344 pKa = 11.07 YY345 pKa = 10.69 LLTKK349 pKa = 10.05 PYY351 pKa = 10.74 SRR353 pKa = 11.84 DD354 pKa = 2.52 HH355 pKa = 6.41 HH356 pKa = 7.1 AYY358 pKa = 8.18 HH359 pKa = 6.29 QCMKK363 pKa = 10.65 QLTTTSGVSYY373 pKa = 10.45 FGTRR377 pKa = 11.84 YY378 pKa = 9.08 KK379 pKa = 11.1 VKK381 pKa = 9.35 GTRR384 pKa = 11.84 CSGDD388 pKa = 2.7 AHH390 pKa = 6.14 TSLGNGLINRR400 pKa = 11.84 FIIWLCLRR408 pKa = 11.84 KK409 pKa = 9.8 LPQNAWDD416 pKa = 4.09 SMHH419 pKa = 6.9 EE420 pKa = 4.16 GDD422 pKa = 5.56 DD423 pKa = 4.41 GIIGVSLSYY432 pKa = 11.23 VNQAVYY438 pKa = 10.86 NLQFLACLGFEE449 pKa = 4.64 SKK451 pKa = 10.96 LKK453 pKa = 8.54 VCKK456 pKa = 10.46 SIEE459 pKa = 3.96 EE460 pKa = 4.2 VVFCGRR466 pKa = 11.84 RR467 pKa = 11.84 HH468 pKa = 4.7 VRR470 pKa = 11.84 TAKK473 pKa = 10.34 EE474 pKa = 3.76 SATICDD480 pKa = 4.12 VIRR483 pKa = 11.84 TMRR486 pKa = 11.84 KK487 pKa = 9.22 FNTTCSLGPPDD498 pKa = 4.42 LLLYY502 pKa = 10.88 AKK504 pKa = 10.12 ALSYY508 pKa = 11.18 NYY510 pKa = 9.6 TDD512 pKa = 4.21 ADD514 pKa = 3.95 TPIIGPVSYY523 pKa = 10.96 AVARR527 pKa = 11.84 CLAHH531 pKa = 7.1 CNIKK535 pKa = 10.52 YY536 pKa = 9.98 SRR538 pKa = 11.84 KK539 pKa = 7.68 QFKK542 pKa = 10.09 RR543 pKa = 11.84 ALKK546 pKa = 8.84 TAVLEE551 pKa = 4.12 RR552 pKa = 11.84 WVLNDD557 pKa = 3.48 NIGSISYY564 pKa = 9.96 HH565 pKa = 6.22 RR566 pKa = 11.84 LLNAKK571 pKa = 9.46 PPDD574 pKa = 3.4 ISPYY578 pKa = 10.54 LITASLMLNRR588 pKa = 11.84 PTSVQKK594 pKa = 10.97 LMQQQ598 pKa = 3.14
Molecular weight: 68.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.906
IPC2_protein 8.96
IPC_protein 8.843
Toseland 9.56
ProMoST 9.341
Dawson 9.838
Bjellqvist 9.589
Wikipedia 9.999
Rodwell 10.16
Grimsley 9.911
Solomon 9.853
Lehninger 9.809
Nozaki 9.736
DTASelect 9.531
Thurlkill 9.692
EMBOSS 9.999
Sillero 9.794
Patrickios 5.08
IPC_peptide 9.853
IPC2_peptide 8.346
IPC2.peptide.svr19 7.957
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1448
598
850
724.0
81.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.423 ± 0.345
2.279 ± 0.139
5.525 ± 0.409
4.213 ± 0.324
3.384 ± 0.077
4.972 ± 0.379
3.867 ± 0.317
6.423 ± 0.548
5.525 ± 1.213
8.425 ± 1.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.141 ± 0.425
3.729 ± 0.071
6.215 ± 0.388
4.282 ± 0.467
7.528 ± 0.711
8.011 ± 0.7
7.666 ± 0.288
5.18 ± 0.307
1.174 ± 0.099
3.039 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here