Kanyawara virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ledantevirus; Kanyawara ledantevirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220FHW5|A0A220FHW5_9RHAB Phosphoprotein OS=Kanyawara virus OX=2014931 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.27FNRR5 pKa = 11.84SKK7 pKa = 10.22IHH9 pKa = 5.85SAASRR14 pKa = 11.84LSWSAVNTNLGGLEE28 pKa = 3.89EE29 pKa = 4.54DD30 pKa = 3.51AEE32 pKa = 4.44EE33 pKa = 4.85IILEE37 pKa = 4.13NSSSVKK43 pKa = 10.36EE44 pKa = 4.2ADD46 pKa = 3.14QSAYY50 pKa = 10.9SSWANNPVIEE60 pKa = 4.3TLDD63 pKa = 3.83EE64 pKa = 4.37IFSEE68 pKa = 5.5DD69 pKa = 4.21SDD71 pKa = 3.94TDD73 pKa = 3.72TEE75 pKa = 4.44KK76 pKa = 10.9QSSSQNSKK84 pKa = 8.02STEE87 pKa = 4.15DD88 pKa = 3.5IVCKK92 pKa = 10.3EE93 pKa = 4.29SNSTEE98 pKa = 4.01DD99 pKa = 3.64VIEE102 pKa = 4.14SLKK105 pKa = 11.05KK106 pKa = 9.12EE107 pKa = 4.36DD108 pKa = 3.18EE109 pKa = 4.05RR110 pKa = 11.84QRR112 pKa = 11.84AGKK115 pKa = 9.39KK116 pKa = 9.51DD117 pKa = 3.33KK118 pKa = 11.02RR119 pKa = 11.84SITLIIPNISRR130 pKa = 11.84IHH132 pKa = 5.11NQRR135 pKa = 11.84MLDD138 pKa = 3.78DD139 pKa = 4.47VIKK142 pKa = 10.61EE143 pKa = 3.78ITNNLVEE150 pKa = 4.17QLGFQTYY157 pKa = 9.45PDD159 pKa = 3.56LMEE162 pKa = 5.71INDD165 pKa = 4.51RR166 pKa = 11.84SLKK169 pKa = 10.65LYY171 pKa = 10.67VSADD175 pKa = 3.22NDD177 pKa = 3.71VATSASKK184 pKa = 11.02VSLDD188 pKa = 3.61SKK190 pKa = 9.7PTISTLPQNSPEE202 pKa = 4.02SLIDD206 pKa = 3.45RR207 pKa = 11.84FRR209 pKa = 11.84TGITFQKK216 pKa = 10.37RR217 pKa = 11.84RR218 pKa = 11.84GGDD221 pKa = 3.35LKK223 pKa = 10.17ITLSSGRR230 pKa = 11.84ITEE233 pKa = 5.24LMILDD238 pKa = 5.17CYY240 pKa = 10.66SAAANEE246 pKa = 4.21DD247 pKa = 3.68DD248 pKa = 5.8AIRR251 pKa = 11.84TILKK255 pKa = 9.77KK256 pKa = 10.83ANLYY260 pKa = 7.05TTLTLLTNYY269 pKa = 9.94KK270 pKa = 10.44

Molecular weight: 30.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220FHW5|A0A220FHW5_9RHAB Phosphoprotein OS=Kanyawara virus OX=2014931 PE=4 SV=1
MM1 pKa = 7.26LQRR4 pKa = 11.84FKK6 pKa = 11.24KK7 pKa = 8.16HH8 pKa = 5.69TKK10 pKa = 10.14LKK12 pKa = 9.2TPSAPSQSTIYY23 pKa = 10.81GLSTKK28 pKa = 9.46WDD30 pKa = 3.53LHH32 pKa = 5.67TNPQIDD38 pKa = 3.84NVVQPYY44 pKa = 9.46RR45 pKa = 11.84VEE47 pKa = 3.8EE48 pKa = 3.92TLRR51 pKa = 11.84IQNKK55 pKa = 9.79LEE57 pKa = 3.82IRR59 pKa = 11.84AKK61 pKa = 10.53EE62 pKa = 4.02PLRR65 pKa = 11.84DD66 pKa = 3.54LTDD69 pKa = 3.63CLKK72 pKa = 10.51IAEE75 pKa = 4.23VWIDD79 pKa = 3.85EE80 pKa = 4.32QNCPYY85 pKa = 8.96WQVGIDD91 pKa = 3.3TWIFICMALHH101 pKa = 5.53AQKK104 pKa = 10.69DD105 pKa = 4.29GSCRR109 pKa = 11.84YY110 pKa = 7.88IHH112 pKa = 7.65LYY114 pKa = 8.42RR115 pKa = 11.84TQLDD119 pKa = 3.45QVVDD123 pKa = 4.85FKK125 pKa = 11.0IQRR128 pKa = 11.84PFLEE132 pKa = 4.67RR133 pKa = 11.84INYY136 pKa = 7.89KK137 pKa = 10.02KK138 pKa = 9.98HH139 pKa = 4.3SQYY142 pKa = 11.45YY143 pKa = 6.31EE144 pKa = 3.96TTHH147 pKa = 6.82RR148 pKa = 11.84GSTCSVSYY156 pKa = 10.37EE157 pKa = 4.32SNLALSKK164 pKa = 10.1RR165 pKa = 11.84TGIPSHH171 pKa = 6.47VLYY174 pKa = 10.41QYY176 pKa = 10.11PLKK179 pKa = 10.93DD180 pKa = 3.47GNSPPKK186 pKa = 10.37FDD188 pKa = 4.93DD189 pKa = 4.59LGIKK193 pKa = 8.81TPIVVTYY200 pKa = 10.66ADD202 pKa = 5.06DD203 pKa = 4.32GSQIIGTKK211 pKa = 9.93

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3573

211

2117

714.6

81.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.618 ± 0.435

1.959 ± 0.361

5.682 ± 0.5

5.57 ± 0.676

3.834 ± 0.463

5.43 ± 0.589

2.883 ± 0.449

7.529 ± 0.582

6.465 ± 0.282

10.327 ± 0.652

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.276

5.43 ± 0.348

4.086 ± 0.365

3.61 ± 0.481

5.038 ± 0.337

8.34 ± 0.89

6.297 ± 0.572

5.038 ± 0.419

1.931 ± 0.228

3.638 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski