Victivallis vadensis
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4059 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1ANM2|A0A2U1ANM2_9BACT Prepilin-type N-terminal cleavage/methylation domain-containing protein/prepilin-type processing-associated H-X9-DG protein OS=Victivallis vadensis OX=172901 GN=C8D82_1297 PE=4 SV=1
MM1 pKa = 8.03 DD2 pKa = 4.83 EE3 pKa = 4.15 SLKK6 pKa = 10.94 FISFVVNYY14 pKa = 9.64 CRR16 pKa = 11.84 NTLIDD21 pKa = 4.18 FYY23 pKa = 10.41 PYY25 pKa = 10.05 QQGHH29 pKa = 5.85 EE30 pKa = 4.37 KK31 pKa = 10.68 LNEE34 pKa = 4.63 DD35 pKa = 3.77 IYY37 pKa = 11.06 GMKK40 pKa = 10.2 YY41 pKa = 10.17 YY42 pKa = 10.73 SEE44 pKa = 3.93 DD45 pKa = 3.88 HH46 pKa = 6.3 EE47 pKa = 4.5 WVEE50 pKa = 4.45 IVGDD54 pKa = 3.84 EE55 pKa = 4.12 ATVGISEE62 pKa = 4.45 YY63 pKa = 11.13 AVDD66 pKa = 4.01 EE67 pKa = 5.07 LGDD70 pKa = 3.4 ITYY73 pKa = 10.29 VEE75 pKa = 4.5 LPEE78 pKa = 4.87 EE79 pKa = 4.34 EE80 pKa = 4.87 DD81 pKa = 4.0 DD82 pKa = 5.61 FIIGDD87 pKa = 3.58 RR88 pKa = 11.84 LGEE91 pKa = 4.17 VEE93 pKa = 4.64 SVNSSSEE100 pKa = 3.79 IYY102 pKa = 10.58 SPISGTVSQVNEE114 pKa = 4.11 ALADD118 pKa = 3.8 EE119 pKa = 5.19 PGLINEE125 pKa = 4.36 SPEE128 pKa = 4.12 DD129 pKa = 3.78 KK130 pKa = 10.89 GWLCRR135 pKa = 11.84 LTNFDD140 pKa = 4.44 SSEE143 pKa = 4.35 LDD145 pKa = 3.53 DD146 pKa = 4.92 MMNEE150 pKa = 4.21 DD151 pKa = 5.1 AYY153 pKa = 11.26 QKK155 pKa = 10.35 YY156 pKa = 9.82 LRR158 pKa = 11.84 KK159 pKa = 9.67 LRR161 pKa = 11.84 RR162 pKa = 3.33
Molecular weight: 18.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.745
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.783
Rodwell 3.757
Grimsley 3.656
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.164
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.05
Patrickios 1.888
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.94
Protein with the highest isoelectric point:
>tr|A0A2U1AE99|A0A2U1AE99_9BACT AlpA family transcriptional regulator OS=Victivallis vadensis OX=172901 GN=C8D82_1485 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 9.41 RR14 pKa = 11.84 RR15 pKa = 11.84 IGFFARR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 LVIKK32 pKa = 10.44 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.71 GRR39 pKa = 11.84 AKK41 pKa = 9.64 LTAA44 pKa = 4.21
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4059
0
4059
1560380
29
3328
384.4
42.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.075 ± 0.04
1.484 ± 0.016
5.135 ± 0.025
6.64 ± 0.035
4.576 ± 0.025
8.07 ± 0.042
1.833 ± 0.015
5.058 ± 0.029
4.32 ± 0.032
10.136 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.251 ± 0.017
3.612 ± 0.029
5.264 ± 0.033
2.871 ± 0.021
7.253 ± 0.044
5.426 ± 0.027
4.802 ± 0.027
6.529 ± 0.033
1.491 ± 0.016
3.174 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here