Streptococcus phage phi-SC181

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K537|A0A5C1K537_9CAUD XRE family transcriptional regulator OS=Streptococcus phage phi-SC181 OX=2607969 PE=3 SV=1
MM1 pKa = 7.68ALLNIDD7 pKa = 3.96KK8 pKa = 9.38VTEE11 pKa = 4.25PFDD14 pKa = 4.53LEE16 pKa = 4.1TALTYY21 pKa = 9.39MRR23 pKa = 11.84KK24 pKa = 8.48NGEE27 pKa = 4.18FIRR30 pKa = 11.84CKK32 pKa = 9.37TAEE35 pKa = 3.98QDD37 pKa = 3.13FYY39 pKa = 11.24MYY41 pKa = 10.43LEE43 pKa = 4.31EE44 pKa = 4.27VRR46 pKa = 11.84RR47 pKa = 11.84PAIKK51 pKa = 9.97NGKK54 pKa = 7.82RR55 pKa = 11.84QLVTTEE61 pKa = 4.17TVWAFNQWGSTTLTLNLSDD80 pKa = 5.06LFHH83 pKa = 7.49DD84 pKa = 4.76CFYY87 pKa = 11.68LMRR90 pKa = 11.84FDD92 pKa = 5.99EE93 pKa = 4.69NGQPDD98 pKa = 3.81WSDD101 pKa = 3.11PTIVQEE107 pKa = 4.44GSLEE111 pKa = 4.28SEE113 pKa = 4.4VTDD116 pKa = 3.45EE117 pKa = 4.36GTVNSS122 pKa = 4.39

Molecular weight: 14.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K5Z9|A0A5C1K5Z9_9CAUD Adenine-specific methyltransferase or lactate dehydrogenase OS=Streptococcus phage phi-SC181 OX=2607969 PE=3 SV=1
MM1 pKa = 7.25TMNHH5 pKa = 6.18FKK7 pKa = 10.83GKK9 pKa = 9.64QFQQDD14 pKa = 3.74VIIVAVGYY22 pKa = 7.45YY23 pKa = 10.31LRR25 pKa = 11.84YY26 pKa = 7.98NLSYY30 pKa = 11.11RR31 pKa = 11.84EE32 pKa = 4.06VQEE35 pKa = 3.58ILYY38 pKa = 10.59DD39 pKa = 3.47RR40 pKa = 11.84GINVSHH46 pKa = 4.71TTIYY50 pKa = 10.54RR51 pKa = 11.84WVQEE55 pKa = 4.1YY56 pKa = 11.0GKK58 pKa = 10.52LLYY61 pKa = 10.23QIWKK65 pKa = 9.5KK66 pKa = 10.61KK67 pKa = 8.69NKK69 pKa = 9.61KK70 pKa = 9.91SFYY73 pKa = 8.3SWKK76 pKa = 9.54MDD78 pKa = 3.12EE79 pKa = 4.49TYY81 pKa = 10.87IKK83 pKa = 10.38IKK85 pKa = 10.7VKK87 pKa = 8.58WHH89 pKa = 4.98YY90 pKa = 10.56LYY92 pKa = 10.73RR93 pKa = 11.84AIDD96 pKa = 3.83ADD98 pKa = 4.65GLTLDD103 pKa = 2.58IWLRR107 pKa = 11.84KK108 pKa = 9.64KK109 pKa = 10.6RR110 pKa = 11.84DD111 pKa = 3.33TQAAYY116 pKa = 10.45AFLKK120 pKa = 10.75RR121 pKa = 11.84LVKK124 pKa = 10.68QFDD127 pKa = 3.71EE128 pKa = 4.65PKK130 pKa = 10.87VVVTDD135 pKa = 4.14KK136 pKa = 11.36APSITSAFKK145 pKa = 10.54KK146 pKa = 9.62LKK148 pKa = 10.45EE149 pKa = 3.78YY150 pKa = 10.98GFYY153 pKa = 10.24QGTEE157 pKa = 3.58HH158 pKa = 7.12RR159 pKa = 11.84TIKK162 pKa = 10.51YY163 pKa = 9.93LNNLIEE169 pKa = 4.19QDD171 pKa = 3.23HH172 pKa = 6.46RR173 pKa = 11.84PVKK176 pKa = 10.29RR177 pKa = 11.84RR178 pKa = 11.84NKK180 pKa = 9.7FYY182 pKa = 10.91RR183 pKa = 11.84SLRR186 pKa = 11.84TASTTIKK193 pKa = 10.47GMEE196 pKa = 4.94AIRR199 pKa = 11.84GLYY202 pKa = 10.03KK203 pKa = 9.75KK204 pKa = 7.98TRR206 pKa = 11.84KK207 pKa = 9.8EE208 pKa = 3.62GTLFGFSVCTEE219 pKa = 3.8IKK221 pKa = 10.5VLLGIPAA228 pKa = 4.29

Molecular weight: 27.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

11954

85

1515

412.2

46.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.157 ± 0.393

1.054 ± 0.163

6.132 ± 0.28

7.06 ± 0.479

4.015 ± 0.262

6.492 ± 0.336

1.916 ± 0.129

6.868 ± 0.315

7.395 ± 0.28

8.675 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.24

5.078 ± 0.273

2.986 ± 0.188

4.258 ± 0.257

4.793 ± 0.201

6.425 ± 0.442

6.199 ± 0.552

6.533 ± 0.302

1.313 ± 0.125

4.291 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski