Anaerostipes hadrus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerostipes

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4MWL6|D4MWL6_ANAHA DUF1653 domain-containing protein OS=Anaerostipes hadrus OX=649756 GN=CL2_30100 PE=4 SV=1
MM1 pKa = 8.15PDD3 pKa = 3.05NLFPEE8 pKa = 4.92EE9 pKa = 4.21YY10 pKa = 9.78EE11 pKa = 4.0NEE13 pKa = 3.92EE14 pKa = 4.47EE15 pKa = 4.33YY16 pKa = 11.19FEE18 pKa = 5.9DD19 pKa = 4.21EE20 pKa = 4.35EE21 pKa = 5.03NEE23 pKa = 4.07GTEE26 pKa = 4.12EE27 pKa = 4.08EE28 pKa = 4.28NTEE31 pKa = 4.02EE32 pKa = 4.85EE33 pKa = 4.09EE34 pKa = 4.23DD35 pKa = 3.44AGYY38 pKa = 10.3KK39 pKa = 10.17PSIFFDD45 pKa = 3.9FDD47 pKa = 3.11TGDD50 pKa = 3.34FVTLHH55 pKa = 6.64DD56 pKa = 4.82GKK58 pKa = 11.02LKK60 pKa = 10.04EE61 pKa = 4.16ASGFEE66 pKa = 4.89AWVQWCYY73 pKa = 9.26KK74 pKa = 10.17TIMTQRR80 pKa = 11.84YY81 pKa = 7.16AHH83 pKa = 6.79EE84 pKa = 5.51GYY86 pKa = 8.36STDD89 pKa = 3.2IGIDD93 pKa = 3.59YY94 pKa = 10.44EE95 pKa = 4.48SALQADD101 pKa = 4.01SRR103 pKa = 11.84EE104 pKa = 4.12EE105 pKa = 4.0AEE107 pKa = 5.22SILQRR112 pKa = 11.84EE113 pKa = 4.25IEE115 pKa = 4.35EE116 pKa = 4.57ALMADD121 pKa = 3.41PSEE124 pKa = 4.05RR125 pKa = 11.84TLYY128 pKa = 10.25VGNIMFQWEE137 pKa = 4.34AEE139 pKa = 4.22HH140 pKa = 6.94CLVTVQVQGIDD151 pKa = 3.39GDD153 pKa = 4.36IEE155 pKa = 3.96IQTQFEE161 pKa = 4.77SEE163 pKa = 4.65VVV165 pKa = 3.2

Molecular weight: 19.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4MX64|D4MX64_ANAHA tRNA modification GTPase MnmE OS=Anaerostipes hadrus OX=649756 GN=mnmE PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.25VLKK32 pKa = 10.25SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84NRR41 pKa = 11.84LSAA44 pKa = 3.84

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2771

0

2771

812062

13

2106

293.1

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.781 ± 0.048

1.396 ± 0.019

5.799 ± 0.036

7.522 ± 0.053

4.09 ± 0.035

6.828 ± 0.041

1.844 ± 0.021

8.075 ± 0.05

8.407 ± 0.055

8.507 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.257 ± 0.026

4.485 ± 0.034

3.01 ± 0.026

3.311 ± 0.029

3.859 ± 0.033

5.585 ± 0.038

5.435 ± 0.043

6.798 ± 0.038

0.807 ± 0.016

4.202 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski