Musa acuminata subsp. malaccensis (Wild banana) (Musa malaccensis)
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36474 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M0U7Q2|M0U7Q2_MUSAM Uncharacterized protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103973565 PE=4 SV=1
MM1 pKa = 7.94 ASLLLLLSMASLLLSPLSLADD22 pKa = 3.99 PNATTMPTPSGEE34 pKa = 4.22 IQRR37 pKa = 11.84 EE38 pKa = 3.58 MDD40 pKa = 3.41 QQILATVLPDD50 pKa = 3.53 SSSNTQPFLTSPTGKK65 pKa = 8.31 YY66 pKa = 7.94 TSYY69 pKa = 11.41 LLRR72 pKa = 11.84 HH73 pKa = 5.52 KK74 pKa = 7.21 TTPEE78 pKa = 3.6 SGGYY82 pKa = 10.48 GSDD85 pKa = 3.04 FCYY88 pKa = 10.39 IQIQDD93 pKa = 3.96 TVSGDD98 pKa = 4.05 SVWEE102 pKa = 4.32 SEE104 pKa = 4.39 CEE106 pKa = 4.06 PVSSANACTLVFSDD120 pKa = 3.72 TGLAVLDD127 pKa = 4.81 GSQSVWDD134 pKa = 4.0 TGASNGNNFPATLEE148 pKa = 4.03 LADD151 pKa = 5.06 LGDD154 pKa = 3.79 MMVADD159 pKa = 4.89 KK160 pKa = 11.14 DD161 pKa = 4.21 GEE163 pKa = 4.63 LVWKK167 pKa = 10.64 ASDD170 pKa = 3.93 DD171 pKa = 3.46 PRR173 pKa = 11.84 VNQGCGSPDD182 pKa = 3.6 LGSGSPTFVGGDD194 pKa = 3.46 DD195 pKa = 4.22 SSPLGQQLPPPPPSLSEE212 pKa = 3.99 GALPLAPASPPVVGDD227 pKa = 4.23 DD228 pKa = 3.93 NLTSGQAPLLAPTSAPFAAPTSGDD252 pKa = 3.17 EE253 pKa = 4.25 NIPYY257 pKa = 9.18 NQQPPSYY264 pKa = 9.15 SSNVAFGQQQEE275 pKa = 4.18 QQMSAFHH282 pKa = 6.53 GLHH285 pKa = 6.13 GVNQPLVDD293 pKa = 3.73 NNAYY297 pKa = 10.49 DD298 pKa = 3.66 SGCSRR303 pKa = 11.84 KK304 pKa = 9.79 EE305 pKa = 3.65 GLIGILLVVVSHH317 pKa = 6.5 LVLRR321 pKa = 11.84 GLL323 pKa = 4.07
Molecular weight: 33.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 1.163
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|M0UB60|M0UB60_MUSAM IPPc domain-containing protein OS=Musa acuminata subsp. malaccensis OX=214687 PE=3 SV=1
MM1 pKa = 7.91 VMASTSAAQQVLRR14 pKa = 11.84 GGSLSSPPPPRR25 pKa = 11.84 RR26 pKa = 11.84 SPGSRR31 pKa = 11.84 RR32 pKa = 11.84 SRR34 pKa = 11.84 AVATSSTRR42 pKa = 11.84 NGLMARR48 pKa = 11.84 SRR50 pKa = 11.84 VTTGSTRR57 pKa = 11.84 LASARR62 pKa = 11.84 TRR64 pKa = 11.84 PSS66 pKa = 2.71
Molecular weight: 6.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.281
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36474
0
36474
12599352
10
12011
345.4
38.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.616 ± 0.017
1.987 ± 0.007
5.209 ± 0.009
6.14 ± 0.015
4.058 ± 0.01
6.741 ± 0.014
2.511 ± 0.007
4.968 ± 0.011
5.345 ± 0.015
9.876 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.006
3.789 ± 0.01
5.149 ± 0.013
3.534 ± 0.011
5.984 ± 0.013
8.902 ± 0.02
4.757 ± 0.009
6.509 ± 0.011
1.326 ± 0.005
2.711 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here