Desulfovibrio sp. X2
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3444 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S7VAY5|S7VAY5_9DELT Uncharacterized protein OS=Desulfovibrio sp. X2 OX=941449 GN=dsx2_0088 PE=4 SV=1
MM1 pKa = 7.5 SLTGSLYY8 pKa = 10.86 AGISGLQAHH17 pKa = 5.6 SQKK20 pKa = 10.22 MSVIGNNLANTSTIGFKK37 pKa = 10.64 SSTMQFEE44 pKa = 5.14 DD45 pKa = 3.43 IFYY48 pKa = 10.69 QSVNTGAGIGQVGVGAGVAAIYY70 pKa = 11.02 SNFAQGSYY78 pKa = 10.48 EE79 pKa = 4.24 STSEE83 pKa = 4.03 ATDD86 pKa = 3.38 VAIGGTGFFMVNNPNTDD103 pKa = 2.65 EE104 pKa = 4.1 MYY106 pKa = 8.9 YY107 pKa = 9.98 TRR109 pKa = 11.84 AGEE112 pKa = 3.95 FRR114 pKa = 11.84 FDD116 pKa = 3.23 NSGYY120 pKa = 10.78 LVDD123 pKa = 3.78 TNGYY127 pKa = 8.89 RR128 pKa = 11.84 VQGWQVKK135 pKa = 9.81 DD136 pKa = 3.87 GSSSGTVQTTGVSGDD151 pKa = 3.32 IQLKK155 pKa = 9.89 NFQSPPEE162 pKa = 4.13 ATSTVSMLLNLDD174 pKa = 3.79 SSSDD178 pKa = 3.74 DD179 pKa = 3.94 DD180 pKa = 4.28 SANAANPFFSLFGNWDD196 pKa = 3.49 GTDD199 pKa = 3.37 SDD201 pKa = 4.44 TPIADD206 pKa = 3.35 SRR208 pKa = 11.84 YY209 pKa = 9.74 AYY211 pKa = 10.69 QNTITVYY218 pKa = 10.78 DD219 pKa = 4.2 EE220 pKa = 4.38 NGSAQTLTVYY230 pKa = 10.72 CDD232 pKa = 3.53 PVKK235 pKa = 10.71 DD236 pKa = 3.7 ASVVSNSGGNLQWEE250 pKa = 5.19 FIVACDD256 pKa = 3.57 PSEE259 pKa = 4.43 DD260 pKa = 3.3 GRR262 pKa = 11.84 TIGGQKK268 pKa = 10.1 VGSTSAAGLLMTGTLTFNAAGQLTGMSAYY297 pKa = 8.93 TLASGASGDD306 pKa = 5.44 LKK308 pKa = 11.14 DD309 pKa = 4.11 LSNWTTADD317 pKa = 4.0 LNDD320 pKa = 4.93 DD321 pKa = 3.92 GLPTFTANFTGEE333 pKa = 4.03 ADD335 pKa = 3.36 ASATGEE341 pKa = 4.14 ANASAIAIDD350 pKa = 4.69 FGIHH354 pKa = 7.31 DD355 pKa = 4.7 SGSPTSWSGSASNASMVGNNPSMLDD380 pKa = 3.15 NFTSPKK386 pKa = 9.91 ISALTTTSYY395 pKa = 11.29 DD396 pKa = 3.4 SSSTTISQSQDD407 pKa = 2.54 GYY409 pKa = 11.72 GPGFLTDD416 pKa = 3.37 ISVNRR421 pKa = 11.84 DD422 pKa = 2.85 GVITGTYY429 pKa = 10.73 SNGQVLDD436 pKa = 4.39 LYY438 pKa = 11.38 VLTLASFTNPYY449 pKa = 9.8 GLSRR453 pKa = 11.84 EE454 pKa = 3.92 GDD456 pKa = 3.55 NLFSATRR463 pKa = 11.84 EE464 pKa = 3.79 SGAAVTGTANTGQLGSISSNTLEE487 pKa = 4.14 QSNVDD492 pKa = 3.52 TATEE496 pKa = 4.09 MVDD499 pKa = 5.24 LITTQRR505 pKa = 11.84 GFEE508 pKa = 4.22 ANSKK512 pKa = 10.68 VITTADD518 pKa = 3.25 SMLSEE523 pKa = 5.48 LIQLKK528 pKa = 10.17 RR529 pKa = 3.41
Molecular weight: 55.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.617
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.228
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.973
Patrickios 2.486
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|S7V9I9|S7V9I9_9DELT Acetolactate synthase OS=Desulfovibrio sp. X2 OX=941449 GN=dsx2_2403 PE=4 SV=1
MM1 pKa = 7.66 SSRR4 pKa = 11.84 LTLVIRR10 pKa = 11.84 VDD12 pKa = 4.33 FKK14 pKa = 10.19 TRR16 pKa = 11.84 RR17 pKa = 11.84 VLAVFGGRR25 pKa = 11.84 RR26 pKa = 11.84 SRR28 pKa = 11.84 LAPAAPRR35 pKa = 11.84 APLPAHH41 pKa = 7.15 LLRR44 pKa = 11.84 LTTGAATASQGGAQQ58 pKa = 3.48
Molecular weight: 6.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.362
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.106
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.857
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3444
0
3444
1149838
32
1807
333.9
36.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.984 ± 0.065
1.344 ± 0.018
5.406 ± 0.028
6.493 ± 0.042
3.817 ± 0.026
8.652 ± 0.035
2.027 ± 0.02
4.271 ± 0.035
3.678 ± 0.039
11.096 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.533 ± 0.017
2.328 ± 0.021
5.227 ± 0.032
2.895 ± 0.026
7.321 ± 0.044
5.338 ± 0.028
4.718 ± 0.026
7.392 ± 0.033
1.185 ± 0.015
2.295 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here