Mameliella alba
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5714 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B3RIY3|A0A0B3RIY3_9RHOB Uncharacterized protein OS=Mameliella alba OX=561184 GN=GGR29_000948 PE=4 SV=1
MM1 pKa = 7.78 CFLCQSLDD9 pKa = 3.68 PSIEE13 pKa = 3.87 IYY15 pKa = 10.45 DD16 pKa = 3.78 GHH18 pKa = 7.15 GLTGPTAAPSGANGGTTEE36 pKa = 3.84 AALPVFTLDD45 pKa = 3.18 QVAYY49 pKa = 10.05 QLTHH53 pKa = 7.01 GYY55 pKa = 7.25 WQNGGGDD62 pKa = 2.99 WRR64 pKa = 11.84 AFDD67 pKa = 3.64 VQAGGEE73 pKa = 4.0 ITVNIDD79 pKa = 3.09 ALDD82 pKa = 4.01 AAGQTAALAALEE94 pKa = 4.16 AWTAVSGIQFTLTSGTADD112 pKa = 2.79 ITFDD116 pKa = 3.63 DD117 pKa = 4.57 TEE119 pKa = 5.08 SGAYY123 pKa = 9.58 AYY125 pKa = 10.66 SYY127 pKa = 11.52 VYY129 pKa = 10.14 TSWNEE134 pKa = 3.36 IDD136 pKa = 3.35 QSFVNVHH143 pKa = 6.59 PSWQGYY149 pKa = 8.29 GDD151 pKa = 4.26 YY152 pKa = 10.93 YY153 pKa = 9.0 YY154 pKa = 9.58 QTYY157 pKa = 8.98 IHH159 pKa = 7.24 EE160 pKa = 4.73 IGHH163 pKa = 6.46 ALGLGHH169 pKa = 7.18 GGNYY173 pKa = 9.87 NGAADD178 pKa = 4.89 FGTDD182 pKa = 2.45 AHH184 pKa = 6.43 YY185 pKa = 11.5 ANDD188 pKa = 3.48 SWQMSIMSYY197 pKa = 9.67 FAQWEE202 pKa = 4.36 NPNVDD207 pKa = 3.05 ASGMYY212 pKa = 10.37 LATPQMADD220 pKa = 2.5 ILAIQNLYY228 pKa = 8.15 GTPDD232 pKa = 3.32 NVHH235 pKa = 6.49 TGNTVYY241 pKa = 11.13 GDD243 pKa = 3.84 NTNLTATGMDD253 pKa = 4.67 LAPGRR258 pKa = 11.84 AVAIFDD264 pKa = 3.56 SSGIDD269 pKa = 3.63 VIDD272 pKa = 4.1 LGSRR276 pKa = 11.84 GHH278 pKa = 6.02 DD279 pKa = 2.91 QRR281 pKa = 11.84 LSLEE285 pKa = 4.24 PEE287 pKa = 3.78 SWSDD291 pKa = 2.82 IDD293 pKa = 5.07 GYY295 pKa = 11.56 AGNFAIARR303 pKa = 11.84 GAVIEE308 pKa = 3.95 NAITGSGDD316 pKa = 3.27 DD317 pKa = 4.83 HH318 pKa = 6.27 ITGNAASNDD327 pKa = 3.63 IQSGAGADD335 pKa = 3.8 TIKK338 pKa = 10.91 GGEE341 pKa = 4.22 GDD343 pKa = 3.75 DD344 pKa = 4.55 TIDD347 pKa = 3.52 GGAGEE352 pKa = 4.56 DD353 pKa = 3.44 VVVFEE358 pKa = 4.82 GASEE362 pKa = 4.25 GFALAWNGALTVTDD376 pKa = 3.95 IDD378 pKa = 4.24 LTDD381 pKa = 4.28 GGDD384 pKa = 3.86 DD385 pKa = 3.99 GTDD388 pKa = 2.92 TLLGVEE394 pKa = 4.55 TLSFTDD400 pKa = 4.24 GAFGTVQDD408 pKa = 4.43 NGTTTLVRR416 pKa = 11.84 LYY418 pKa = 9.21 QTGSNLTAATLEE430 pKa = 3.85 IDD432 pKa = 4.41 VDD434 pKa = 4.37 DD435 pKa = 3.76 THH437 pKa = 6.55 EE438 pKa = 4.14 WEE440 pKa = 4.8 SIARR444 pKa = 11.84 DD445 pKa = 4.07 FTATGQWAKK454 pKa = 8.25 QTNTYY459 pKa = 11.31 DD460 pKa = 3.1 NGRR463 pKa = 11.84 VLEE466 pKa = 4.13 IFYY469 pKa = 10.92 SDD471 pKa = 3.85 GLRR474 pKa = 11.84 TSSTMTDD481 pKa = 3.13 SADD484 pKa = 3.2 VYY486 pKa = 11.37 AWDD489 pKa = 4.75 SYY491 pKa = 10.41 TDD493 pKa = 4.09 GYY495 pKa = 10.54 DD496 pKa = 3.09 ASGARR501 pKa = 11.84 IYY503 pKa = 11.19 NGITWDD509 pKa = 3.39 GGKK512 pKa = 9.98 LVEE515 pKa = 4.42 THH517 pKa = 6.79 FDD519 pKa = 3.32 ADD521 pKa = 3.83 GARR524 pKa = 11.84 TSSLVTDD531 pKa = 4.43 GGDD534 pKa = 3.06 VYY536 pKa = 11.13 GWHH539 pKa = 6.42 TIEE542 pKa = 5.53 RR543 pKa = 11.84 VYY545 pKa = 11.53 DD546 pKa = 3.76 DD547 pKa = 5.28 AGVLTDD553 pKa = 3.6 QTNTYY558 pKa = 11.09 DD559 pKa = 3.96 DD560 pKa = 4.29 GRR562 pKa = 11.84 VQQIVYY568 pKa = 9.5 TEE570 pKa = 4.25 GARR573 pKa = 11.84 SAIHH577 pKa = 5.49 MTDD580 pKa = 3.18 AGDD583 pKa = 3.62 VASWASYY590 pKa = 10.46 SDD592 pKa = 4.57 FYY594 pKa = 11.51 ATATGARR601 pKa = 11.84 TARR604 pKa = 11.84 QMTYY608 pKa = 10.9 DD609 pKa = 4.2 DD610 pKa = 4.04 GRR612 pKa = 11.84 QVEE615 pKa = 4.13 IGFDD619 pKa = 3.64 GGRR622 pKa = 11.84 MVSHH626 pKa = 7.58 LLTDD630 pKa = 4.07 GADD633 pKa = 3.48 DD634 pKa = 4.5 FVWSSIARR642 pKa = 11.84 TWDD645 pKa = 3.08 AEE647 pKa = 4.2 GQLDD651 pKa = 4.35 SQTNTYY657 pKa = 11.15 DD658 pKa = 3.31 DD659 pKa = 4.15 GRR661 pKa = 11.84 VHH663 pKa = 7.27 EE664 pKa = 4.96 IDD666 pKa = 3.63 YY667 pKa = 11.2 ADD669 pKa = 3.81 GVRR672 pKa = 11.84 SSSVLTDD679 pKa = 3.37 TQDD682 pKa = 2.98 AYY684 pKa = 10.92 AWTSATEE691 pKa = 4.22 TYY693 pKa = 9.99 DD694 pKa = 3.23 ASGALVEE701 pKa = 5.81 RR702 pKa = 11.84 VTIWDD707 pKa = 4.54 DD708 pKa = 3.41 GSQDD712 pKa = 3.25 VVTYY716 pKa = 10.71 GDD718 pKa = 3.9 SPVVV722 pKa = 3.24
Molecular weight: 77.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.49
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.554
Grimsley 3.401
Solomon 3.745
Lehninger 3.706
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.706
Sillero 3.859
Patrickios 0.477
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A0B3RZK6|A0A0B3RZK6_9RHOB 8-oxo-dGTP diphosphatase OS=Mameliella alba OX=561184 GN=mutT PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5714
0
5714
1739916
29
4497
304.5
33.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.318 ± 0.045
0.946 ± 0.012
6.257 ± 0.035
6.005 ± 0.028
3.618 ± 0.019
8.972 ± 0.036
2.068 ± 0.016
4.814 ± 0.024
2.878 ± 0.026
10.104 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.747 ± 0.018
2.433 ± 0.02
5.308 ± 0.029
3.163 ± 0.015
6.993 ± 0.034
4.958 ± 0.021
5.466 ± 0.027
7.286 ± 0.028
1.453 ± 0.013
2.213 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here