Arthrobacter phage Elsa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mudcatvirus; unclassified Mudcatvirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222Z6U4|A0A222Z6U4_9CAUD Uncharacterized protein OS=Arthrobacter phage Elsa OX=2024276 GN=SEA_ELSA_75 PE=4 SV=1
MM1 pKa = 6.98VALASRR7 pKa = 11.84YY8 pKa = 6.38VHH10 pKa = 6.88KK11 pKa = 9.1PTEE14 pKa = 4.06VLAVEE19 pKa = 4.14LTEE22 pKa = 5.8DD23 pKa = 3.48NLEE26 pKa = 4.33SVAYY30 pKa = 8.25WVNGDD35 pKa = 3.35IFQNPITSTMQSFFRR50 pKa = 11.84IEE52 pKa = 4.05SRR54 pKa = 11.84DD55 pKa = 3.44SEE57 pKa = 4.78LIAEE61 pKa = 4.4VGDD64 pKa = 3.95IVVLDD69 pKa = 4.19DD70 pKa = 5.77DD71 pKa = 5.02EE72 pKa = 4.85FFAMDD77 pKa = 4.14KK78 pKa = 9.91EE79 pKa = 4.85TFHH82 pKa = 6.7EE83 pKa = 4.64KK84 pKa = 10.65YY85 pKa = 8.91VTNEE89 pKa = 3.35ATNIHH94 pKa = 6.07GVWLNNPFSGG104 pKa = 4.8

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222Z6N7|A0A222Z6N7_9CAUD Major capsid and protease fusion protein OS=Arthrobacter phage Elsa OX=2024276 GN=SEA_ELSA_12 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 9.88TRR4 pKa = 11.84TTMVSLLKK12 pKa = 10.71KK13 pKa = 9.93PLQTFQSIYY22 pKa = 10.93LKK24 pKa = 10.37IAEE27 pKa = 4.38PRR29 pKa = 11.84NIRR32 pKa = 11.84ILLFLAYY39 pKa = 10.42LIFIVIGFNGLAHH52 pKa = 7.15PPRR55 pKa = 11.84SYY57 pKa = 10.84EE58 pKa = 3.83QALGSMNLVFAMFGFVSFGALLGSIAVLPGIWWLEE93 pKa = 3.75RR94 pKa = 11.84SGLLSMGIGVLFYY107 pKa = 11.13VLILHH112 pKa = 6.42EE113 pKa = 4.38RR114 pKa = 11.84HH115 pKa = 6.13ASPFAMGIAFIALLVCAVRR134 pKa = 11.84WLEE137 pKa = 3.5IRR139 pKa = 11.84RR140 pKa = 11.84YY141 pKa = 9.51QLAPRR146 pKa = 11.84EE147 pKa = 4.36GG148 pKa = 3.36

Molecular weight: 16.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

18288

32

1492

188.5

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.191 ± 0.442

0.629 ± 0.101

6.376 ± 0.291

7.125 ± 0.391

3.855 ± 0.206

6.797 ± 0.452

1.914 ± 0.175

6.299 ± 0.243

6.523 ± 0.239

8.033 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.696 ± 0.163

4.763 ± 0.135

3.822 ± 0.193

3.341 ± 0.151

5.206 ± 0.311

6.217 ± 0.244

6.026 ± 0.26

6.983 ± 0.231

1.416 ± 0.107

3.789 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski