Streptococcus phage Javan268

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AW75|A0A4D6AW75_9CAUD Integrase OS=Streptococcus phage Javan268 OX=2548083 GN=Javan268_0001 PE=4 SV=1
MM1 pKa = 6.57STKK4 pKa = 10.84AEE6 pKa = 4.15VLQFAHH12 pKa = 6.55NLANSGMGVDD22 pKa = 3.81NDD24 pKa = 3.99GAYY27 pKa = 7.89GTQCADD33 pKa = 3.64LPCYY37 pKa = 10.38IMRR40 pKa = 11.84QFFDD44 pKa = 3.09VSLYY48 pKa = 10.89GNAYY52 pKa = 10.09DD53 pKa = 4.81LLASAEE59 pKa = 4.27SQGVDD64 pKa = 3.03VRR66 pKa = 11.84YY67 pKa = 10.28DD68 pKa = 3.22VAYY71 pKa = 9.78PEE73 pKa = 4.75AGWIFVKK80 pKa = 10.82SFVAGDD86 pKa = 3.64GVNYY90 pKa = 10.09GHH92 pKa = 7.24TGLTVEE98 pKa = 5.22DD99 pKa = 4.02SDD101 pKa = 3.98GLTVKK106 pKa = 10.0TIEE109 pKa = 4.65QNIDD113 pKa = 3.46GNWDD117 pKa = 3.46FLEE120 pKa = 4.35VGGPARR126 pKa = 11.84YY127 pKa = 9.2HH128 pKa = 5.94EE129 pKa = 4.42RR130 pKa = 11.84TVGEE134 pKa = 3.77IVGYY138 pKa = 9.89IVPPYY143 pKa = 10.62EE144 pKa = 5.56DD145 pKa = 3.42GTDD148 pKa = 3.38DD149 pKa = 3.5VSEE152 pKa = 4.7VEE154 pKa = 4.48TEE156 pKa = 3.94PTTDD160 pKa = 3.51EE161 pKa = 4.09IVLEE165 pKa = 4.39EE166 pKa = 4.16EE167 pKa = 5.07DD168 pKa = 3.65GTFTVGEE175 pKa = 3.66AHH177 pKa = 6.42INVRR181 pKa = 11.84RR182 pKa = 11.84APNLTSDD189 pKa = 3.57VVAVYY194 pKa = 10.35EE195 pKa = 4.23PGEE198 pKa = 4.2TVQYY202 pKa = 10.67DD203 pKa = 3.91SKK205 pKa = 11.58GSANGYY211 pKa = 8.76RR212 pKa = 11.84WISFVGASGSRR223 pKa = 11.84NYY225 pKa = 9.46MAIGQTDD232 pKa = 3.57EE233 pKa = 3.92VGNRR237 pKa = 11.84ITLWGTVDD245 pKa = 3.09

Molecular weight: 26.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B0M2|A0A4D6B0M2_9CAUD Capsid and scaffold protein OS=Streptococcus phage Javan268 OX=2548083 GN=Javan268_0044 PE=4 SV=1
MM1 pKa = 6.64GTNVSVKK8 pKa = 10.09VDD10 pKa = 3.46LKK12 pKa = 11.18GIEE15 pKa = 4.43KK16 pKa = 10.21KK17 pKa = 10.87VSPTALAKK25 pKa = 10.66GKK27 pKa = 9.94LAIANQMLIDD37 pKa = 3.88FTPFVPRR44 pKa = 11.84KK45 pKa = 9.49SGEE48 pKa = 3.93LSGSGQATKK57 pKa = 10.83DD58 pKa = 3.42GVKK61 pKa = 10.61YY62 pKa = 9.36PGPYY66 pKa = 10.14ARR68 pKa = 11.84AQFYY72 pKa = 10.31GSSYY76 pKa = 9.9NKK78 pKa = 9.35VRR80 pKa = 11.84TFVFKK85 pKa = 10.78KK86 pKa = 9.01YY87 pKa = 6.05TTPGTGKK94 pKa = 10.24RR95 pKa = 11.84WDD97 pKa = 3.92LKK99 pKa = 11.32AEE101 pKa = 4.24ALHH104 pKa = 6.29SSEE107 pKa = 3.7WGKK110 pKa = 11.07VGLRR114 pKa = 11.84AMGVKK119 pKa = 10.0AA120 pKa = 4.47

Molecular weight: 13.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10148

39

1169

203.0

22.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.657 ± 1.09

0.404 ± 0.098

6.395 ± 0.371

6.681 ± 0.546

3.922 ± 0.207

6.977 ± 0.535

1.35 ± 0.175

6.642 ± 0.28

7.588 ± 0.489

7.913 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.311

5.439 ± 0.316

3.193 ± 0.29

4.346 ± 0.312

3.981 ± 0.272

6.908 ± 0.57

6.218 ± 0.338

6.504 ± 0.32

1.202 ± 0.168

3.971 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski