butyrate-producing bacterium SS3/4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2993 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7GP80|D7GP80_9FIRM Bifunctional NAD(P)H-hydrate repair enzyme OS=butyrate-producing bacterium SS3/4 OX=245014 GN=nnrE PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.31KK3 pKa = 8.63VTALAMAAVVAASALTGCGSSSTAEE28 pKa = 3.82TTAAATEE35 pKa = 4.24AKK37 pKa = 9.54TEE39 pKa = 3.81AAAADD44 pKa = 3.59STKK47 pKa = 10.63AAKK50 pKa = 10.07EE51 pKa = 3.75DD52 pKa = 3.29GKK54 pKa = 9.49TYY56 pKa = 11.03NVGICQLVQHH66 pKa = 7.09PALDD70 pKa = 3.65AATEE74 pKa = 4.18GFEE77 pKa = 5.09AALKK81 pKa = 10.79DD82 pKa = 3.75KK83 pKa = 11.37LGDD86 pKa = 3.55NVKK89 pKa = 10.35FDD91 pKa = 4.16LQNASGDD98 pKa = 3.91SATCATIINQFVSNDD113 pKa = 2.89VDD115 pKa = 5.54LILANATAALQAAAAGTSEE134 pKa = 4.05IPILGTSITDD144 pKa = 3.22YY145 pKa = 10.35ATALDD150 pKa = 3.6IDD152 pKa = 4.02NWTGTTGLNISGTSDD167 pKa = 4.45LAPLDD172 pKa = 3.6QQAAMLNEE180 pKa = 4.74LFPDD184 pKa = 4.04AKK186 pKa = 10.85NVGLLYY192 pKa = 10.79CSAEE196 pKa = 4.16PNSKK200 pKa = 10.05YY201 pKa = 10.18QATTVKK207 pKa = 10.65GYY209 pKa = 10.75LEE211 pKa = 4.04EE212 pKa = 5.78LGYY215 pKa = 8.66TCKK218 pKa = 10.46EE219 pKa = 3.77YY220 pKa = 10.54TFADD224 pKa = 3.85SNDD227 pKa = 3.42IASVTTTAAGEE238 pKa = 4.01NDD240 pKa = 3.82VLYY243 pKa = 11.18VPTDD247 pKa = 3.2NTAASNAEE255 pKa = 4.28IINNICLPAKK265 pKa = 10.07VPVIAGEE272 pKa = 3.92EE273 pKa = 5.06GICSGCGVATLSIDD287 pKa = 3.79YY288 pKa = 11.02YY289 pKa = 11.19DD290 pKa = 3.63IGYY293 pKa = 9.54KK294 pKa = 9.96AGEE297 pKa = 4.16MGAEE301 pKa = 3.68ILMDD305 pKa = 3.96GKK307 pKa = 10.15DD308 pKa = 3.7VKK310 pKa = 10.86EE311 pKa = 4.09MPIEE315 pKa = 4.18FAPEE319 pKa = 3.66VTKK322 pKa = 10.53KK323 pKa = 10.59YY324 pKa = 11.18NKK326 pKa = 9.95ANCEE330 pKa = 3.91ALGITVPDD338 pKa = 4.17DD339 pKa = 3.36YY340 pKa = 11.89VAIEE344 pKa = 4.0EE345 pKa = 4.29

Molecular weight: 35.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7GRM5|D7GRM5_9FIRM Predicted RNA-binding protein OS=butyrate-producing bacterium SS3/4 OX=245014 GN=CK3_06570 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 7.29NTQRR12 pKa = 11.84KK13 pKa = 7.77RR14 pKa = 11.84VHH16 pKa = 6.19GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 8.88VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2993

0

2993

854591

13

3132

285.5

31.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.172 ± 0.05

1.463 ± 0.019

5.662 ± 0.044

7.551 ± 0.055

4.059 ± 0.036

7.572 ± 0.059

1.755 ± 0.018

6.871 ± 0.042

6.75 ± 0.043

8.785 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.244 ± 0.025

4.108 ± 0.038

3.488 ± 0.026

2.946 ± 0.027

4.599 ± 0.042

5.675 ± 0.039

5.555 ± 0.038

6.959 ± 0.044

0.922 ± 0.015

3.865 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski