Amino acid dipepetide frequency for Clostridium sp. CAG:230

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.104AlaAla: 6.104 ± 0.137	1.016AlaCys: 1.016 ± 0.04	4.415AlaAsp: 4.415 ± 0.088	3.922AlaGlu: 3.922 ± 0.083	3.259AlaPhe: 3.259 ± 0.074	5.318AlaGly: 5.318 ± 0.096	1.076AlaHis: 1.076 ± 0.041	5.466AlaIle: 5.466 ± 0.103	6.628AlaLys: 6.628 ± 0.108	6.448AlaLeu: 6.448 ± 0.093	2.318AlaMet: 2.318 ± 0.062	3.001AlaAsn: 3.001 ± 0.064	1.747AlaPro: 1.747 ± 0.067	2.692AlaGln: 2.692 ± 0.074	2.406AlaArg: 2.406 ± 0.064	4.244AlaSer: 4.244 ± 0.092	3.66AlaThr: 3.66 ± 0.088	5.594AlaVal: 5.594 ± 0.099	0.567AlaTrp: 0.567 ± 0.028	2.856AlaTyr: 2.856 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	0.908CysAla: 0.908 ± 0.04	0.297CysCys: 0.297 ± 0.02	0.836CysAsp: 0.836 ± 0.035	0.844CysGlu: 0.844 ± 0.032	0.748CysPhe: 0.748 ± 0.034	1.255CysGly: 1.255 ± 0.04	0.336CysHis: 0.336 ± 0.024	1.329CysIle: 1.329 ± 0.049	1.101CysLys: 1.101 ± 0.043	1.144CysLeu: 1.144 ± 0.046	0.475CysMet: 0.475 ± 0.027	0.667CysAsn: 0.667 ± 0.026	0.529CysPro: 0.529 ± 0.027	0.432CysGln: 0.432 ± 0.029	0.522CysArg: 0.522 ± 0.028	0.843CysSer: 0.843 ± 0.034	0.836CysThr: 0.836 ± 0.043	1.067CysVal: 1.067 ± 0.042	0.098CysTrp: 0.098 ± 0.011	0.655CysTyr: 0.655 ± 0.036	0.0CysXaa: 0.0 ± 0.0
Asp	4.475AspAla: 4.475 ± 0.084	0.917AspCys: 0.917 ± 0.035	3.079AspAsp: 3.079 ± 0.072	4.247AspGlu: 4.247 ± 0.085	2.515AspPhe: 2.515 ± 0.067	4.077AspGly: 4.077 ± 0.091	1.106AspHis: 1.106 ± 0.042	4.76AspIle: 4.76 ± 0.081	4.007AspLys: 4.007 ± 0.077	4.608AspLeu: 4.608 ± 0.088	1.942AspMet: 1.942 ± 0.055	2.239AspAsn: 2.239 ± 0.05	1.682AspPro: 1.682 ± 0.048	1.966AspGln: 1.966 ± 0.056	2.335AspArg: 2.335 ± 0.07	3.341AspSer: 3.341 ± 0.071	3.522AspThr: 3.522 ± 0.065	3.958AspVal: 3.958 ± 0.071	0.553AspTrp: 0.553 ± 0.023	2.738AspTyr: 2.738 ± 0.065	0.001AspXaa: 0.001 ± 0.001
Glu	4.84GluAla: 4.84 ± 0.089	0.849GluCys: 0.849 ± 0.034	4.152GluAsp: 4.152 ± 0.08	7.049GluGlu: 7.049 ± 0.133	2.309GluPhe: 2.309 ± 0.059	3.593GluGly: 3.593 ± 0.087	1.407GluHis: 1.407 ± 0.048	5.437GluIle: 5.437 ± 0.101	7.32GluLys: 7.32 ± 0.12	5.86GluLeu: 5.86 ± 0.096	2.461GluMet: 2.461 ± 0.062	3.963GluAsn: 3.963 ± 0.084	1.6GluPro: 1.6 ± 0.054	3.299GluGln: 3.299 ± 0.092	2.897GluArg: 2.897 ± 0.078	3.366GluSer: 3.366 ± 0.075	3.359GluThr: 3.359 ± 0.073	4.388GluVal: 4.388 ± 0.098	0.642GluTrp: 0.642 ± 0.029	3.269GluTyr: 3.269 ± 0.071	0.0GluXaa: 0.0 ± 0.0
Phe	3.074PheAla: 3.074 ± 0.061	0.765PheCys: 0.765 ± 0.034	2.566PheAsp: 2.566 ± 0.067	2.723PheGlu: 2.723 ± 0.063	2.029PhePhe: 2.029 ± 0.067	2.869PheGly: 2.869 ± 0.074	0.807PheHis: 0.807 ± 0.035	2.743PheIle: 2.743 ± 0.071	1.945PheLys: 1.945 ± 0.053	3.921PheLeu: 3.921 ± 0.104	1.149PheMet: 1.149 ± 0.048	1.416PheAsn: 1.416 ± 0.043	1.149PhePro: 1.149 ± 0.044	1.278PheGln: 1.278 ± 0.041	1.542PheArg: 1.542 ± 0.05	2.934PheSer: 2.934 ± 0.079	2.213PheThr: 2.213 ± 0.056	3.067PheVal: 3.067 ± 0.077	0.401PheTrp: 0.401 ± 0.026	1.859PheTyr: 1.859 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	4.325GlyAla: 4.325 ± 0.103	1.136GlyCys: 1.136 ± 0.047	3.2GlyAsp: 3.2 ± 0.069	3.827GlyGlu: 3.827 ± 0.077	2.856GlyPhe: 2.856 ± 0.069	3.883GlyGly: 3.883 ± 0.108	1.14GlyHis: 1.14 ± 0.04	5.85GlyIle: 5.85 ± 0.101	5.954GlyLys: 5.954 ± 0.099	5.091GlyLeu: 5.091 ± 0.102	2.394GlyMet: 2.394 ± 0.063	3.065GlyAsn: 3.065 ± 0.078	1.071GlyPro: 1.071 ± 0.035	1.98GlyGln: 1.98 ± 0.053	2.526GlyArg: 2.526 ± 0.066	3.964GlySer: 3.964 ± 0.078	4.013GlyThr: 4.013 ± 0.087	4.489GlyVal: 4.489 ± 0.08	0.607GlyTrp: 0.607 ± 0.036	3.242GlyTyr: 3.242 ± 0.075	0.001GlyXaa: 0.001 ± 0.001
His	1.147HisAla: 1.147 ± 0.043	0.359HisCys: 0.359 ± 0.023	0.992HisAsp: 0.992 ± 0.05	0.992HisGlu: 0.992 ± 0.037	1.01HisPhe: 1.01 ± 0.041	1.258HisGly: 1.258 ± 0.04	0.479HisHis: 0.479 ± 0.033	1.443HisIle: 1.443 ± 0.054	1.211HisLys: 1.211 ± 0.042	1.581HisLeu: 1.581 ± 0.053	0.641HisMet: 0.641 ± 0.025	0.796HisAsn: 0.796 ± 0.037	0.839HisPro: 0.839 ± 0.039	0.573HisGln: 0.573 ± 0.029	0.733HisArg: 0.733 ± 0.034	1.136HisSer: 1.136 ± 0.041	1.044HisThr: 1.044 ± 0.038	1.141HisVal: 1.141 ± 0.041	0.174HisTrp: 0.174 ± 0.017	0.83HisTyr: 0.83 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.218IleAla: 6.218 ± 0.101	1.449IleCys: 1.449 ± 0.05	4.337IleAsp: 4.337 ± 0.08	4.925IleGlu: 4.925 ± 0.099	2.76IlePhe: 2.76 ± 0.081	5.147IleGly: 5.147 ± 0.105	1.515IleHis: 1.515 ± 0.047	4.932IleIle: 4.932 ± 0.099	4.505IleLys: 4.505 ± 0.081	7.091IleLeu: 7.091 ± 0.128	2.008IleMet: 2.008 ± 0.05	3.053IleAsn: 3.053 ± 0.062	3.029IlePro: 3.029 ± 0.062	2.718IleGln: 2.718 ± 0.074	3.55IleArg: 3.55 ± 0.077	4.994IleSer: 4.994 ± 0.104	4.755IleThr: 4.755 ± 0.091	5.183IleVal: 5.183 ± 0.102	0.566IleTrp: 0.566 ± 0.03	2.791IleTyr: 2.791 ± 0.06	0.003IleXaa: 0.003 ± 0.002
Lys	5.993LysAla: 5.993 ± 0.103	0.793LysCys: 0.793 ± 0.035	4.878LysAsp: 4.878 ± 0.088	8.905LysGlu: 8.905 ± 0.137	1.895LysPhe: 1.895 ± 0.055	4.556LysGly: 4.556 ± 0.085	1.2LysHis: 1.2 ± 0.048	5.742LysIle: 5.742 ± 0.107	9.867LysLys: 9.867 ± 0.168	5.559LysLeu: 5.559 ± 0.094	2.661LysMet: 2.661 ± 0.061	4.368LysAsn: 4.368 ± 0.086	1.832LysPro: 1.832 ± 0.056	2.798LysGln: 2.798 ± 0.067	3.273LysArg: 3.273 ± 0.08	3.854LysSer: 3.854 ± 0.09	4.443LysThr: 4.443 ± 0.102	5.094LysVal: 5.094 ± 0.09	0.652LysTrp: 0.652 ± 0.032	3.121LysTyr: 3.121 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	6.303LeuAla: 6.303 ± 0.103	1.548LeuCys: 1.548 ± 0.053	4.793LeuAsp: 4.793 ± 0.096	6.128LeuGlu: 6.128 ± 0.122	3.911LeuPhe: 3.911 ± 0.105	5.281LeuGly: 5.281 ± 0.085	1.726LeuHis: 1.726 ± 0.054	5.534LeuIle: 5.534 ± 0.103	6.146LeuLys: 6.146 ± 0.093	8.314LeuLeu: 8.314 ± 0.155	2.218LeuMet: 2.218 ± 0.066	3.476LeuAsn: 3.476 ± 0.078	3.025LeuPro: 3.025 ± 0.07	3.32LeuGln: 3.32 ± 0.073	3.281LeuArg: 3.281 ± 0.072	6.202LeuSer: 6.202 ± 0.102	4.885LeuThr: 4.885 ± 0.084	5.15LeuVal: 5.15 ± 0.09	0.667LeuTrp: 0.667 ± 0.033	3.391LeuTyr: 3.391 ± 0.083	0.003LeuXaa: 0.003 ± 0.002
Met	2.233MetAla: 2.233 ± 0.059	0.32MetCys: 0.32 ± 0.019	1.902MetAsp: 1.902 ± 0.051	2.409MetGlu: 2.409 ± 0.067	0.992MetPhe: 0.992 ± 0.039	1.877MetGly: 1.877 ± 0.054	0.692MetHis: 0.692 ± 0.032	2.39MetIle: 2.39 ± 0.073	2.571MetLys: 2.571 ± 0.059	2.704MetLeu: 2.704 ± 0.056	0.996MetMet: 0.996 ± 0.042	1.59MetAsn: 1.59 ± 0.047	1.083MetPro: 1.083 ± 0.037	1.432MetGln: 1.432 ± 0.045	1.201MetArg: 1.201 ± 0.039	1.626MetSer: 1.626 ± 0.047	1.923MetThr: 1.923 ± 0.055	1.827MetVal: 1.827 ± 0.048	0.191MetTrp: 0.191 ± 0.015	1.014MetTyr: 1.014 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.614AsnAla: 3.614 ± 0.074	0.666AsnCys: 0.666 ± 0.037	2.558AsnAsp: 2.558 ± 0.065	2.858AsnGlu: 2.858 ± 0.069	1.555AsnPhe: 1.555 ± 0.049	3.628AsnGly: 3.628 ± 0.079	0.895AsnHis: 0.895 ± 0.041	3.468AsnIle: 3.468 ± 0.078	3.033AsnLys: 3.033 ± 0.069	3.653AsnLeu: 3.653 ± 0.078	1.377AsnMet: 1.377 ± 0.051	1.889AsnAsn: 1.889 ± 0.058	1.778AsnPro: 1.778 ± 0.051	1.476AsnGln: 1.476 ± 0.047	1.953AsnArg: 1.953 ± 0.057	2.415AsnSer: 2.415 ± 0.064	2.805AsnThr: 2.805 ± 0.076	3.228AsnVal: 3.228 ± 0.069	0.344AsnTrp: 0.344 ± 0.025	1.898AsnTyr: 1.898 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.089ProAla: 2.089 ± 0.058	0.431ProCys: 0.431 ± 0.026	2.135ProAsp: 2.135 ± 0.061	2.263ProGlu: 2.263 ± 0.061	1.424ProPhe: 1.424 ± 0.048	1.785ProGly: 1.785 ± 0.052	0.485ProHis: 0.485 ± 0.029	2.026ProIle: 2.026 ± 0.056	2.22ProLys: 2.22 ± 0.058	2.342ProLeu: 2.342 ± 0.066	0.777ProMet: 0.777 ± 0.032	1.124ProAsn: 1.124 ± 0.037	0.514ProPro: 0.514 ± 0.031	0.96ProGln: 0.96 ± 0.032	0.832ProArg: 0.832 ± 0.034	1.756ProSer: 1.756 ± 0.054	1.625ProThr: 1.625 ± 0.071	2.614ProVal: 2.614 ± 0.068	0.261ProTrp: 0.261 ± 0.02	1.343ProTyr: 1.343 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.427GlnAla: 2.427 ± 0.067	0.417GlnCys: 0.417 ± 0.026	1.958GlnAsp: 1.958 ± 0.05	3.058GlnGlu: 3.058 ± 0.075	1.23GlnPhe: 1.23 ± 0.042	1.923GlnGly: 1.923 ± 0.051	0.511GlnHis: 0.511 ± 0.026	3.003GlnIle: 3.003 ± 0.07	4.312GlnLys: 4.312 ± 0.103	2.668GlnLeu: 2.668 ± 0.068	1.275GlnMet: 1.275 ± 0.054	2.136GlnAsn: 2.136 ± 0.064	0.835GlnPro: 0.835 ± 0.032	1.435GlnGln: 1.435 ± 0.052	1.397GlnArg: 1.397 ± 0.052	2.034GlnSer: 2.034 ± 0.054	2.139GlnThr: 2.139 ± 0.064	1.96GlnVal: 1.96 ± 0.055	0.288GlnTrp: 0.288 ± 0.024	1.535GlnTyr: 1.535 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.114ArgAla: 2.114 ± 0.055	0.535ArgCys: 0.535 ± 0.025	2.023ArgAsp: 2.023 ± 0.056	3.019ArgGlu: 3.019 ± 0.074	1.824ArgPhe: 1.824 ± 0.057	2.023ArgGly: 2.023 ± 0.055	0.772ArgHis: 0.772 ± 0.031	3.334ArgIle: 3.334 ± 0.076	3.607ArgLys: 3.607 ± 0.078	3.297ArgLeu: 3.297 ± 0.08	1.48ArgMet: 1.48 ± 0.045	1.969ArgAsn: 1.969 ± 0.06	1.062ArgPro: 1.062 ± 0.039	1.655ArgGln: 1.655 ± 0.053	1.686ArgArg: 1.686 ± 0.054	2.16ArgSer: 2.16 ± 0.056	1.984ArgThr: 1.984 ± 0.048	2.452ArgVal: 2.452 ± 0.064	0.301ArgTrp: 0.301 ± 0.02	1.836ArgTyr: 1.836 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	4.187SerAla: 4.187 ± 0.087	0.794SerCys: 0.794 ± 0.035	3.708SerAsp: 3.708 ± 0.067	3.613SerGlu: 3.613 ± 0.078	2.706SerPhe: 2.706 ± 0.072	4.552SerGly: 4.552 ± 0.083	1.044SerHis: 1.044 ± 0.041	4.413SerIle: 4.413 ± 0.093	4.422SerLys: 4.422 ± 0.09	5.275SerLeu: 5.275 ± 0.096	1.941SerMet: 1.941 ± 0.047	2.678SerAsn: 2.678 ± 0.064	1.509SerPro: 1.509 ± 0.05	1.926SerGln: 1.926 ± 0.053	2.275SerArg: 2.275 ± 0.065	4.013SerSer: 4.013 ± 0.122	3.163SerThr: 3.163 ± 0.08	4.549SerVal: 4.549 ± 0.094	0.495SerTrp: 0.495 ± 0.029	2.684SerTyr: 2.684 ± 0.061	0.003SerXaa: 0.003 ± 0.002
Thr	4.457ThrAla: 4.457 ± 0.102	0.653ThrCys: 0.653 ± 0.029	3.43ThrAsp: 3.43 ± 0.075	3.506ThrGlu: 3.506 ± 0.081	2.305ThrPhe: 2.305 ± 0.064	4.28ThrGly: 4.28 ± 0.091	0.847ThrHis: 0.847 ± 0.033	4.654ThrIle: 4.654 ± 0.084	4.562ThrLys: 4.562 ± 0.092	4.849ThrLeu: 4.849 ± 0.091	1.527ThrMet: 1.527 ± 0.045	2.465ThrAsn: 2.465 ± 0.059	1.99ThrPro: 1.99 ± 0.056	2.147ThrGln: 2.147 ± 0.062	1.722ThrArg: 1.722 ± 0.041	3.245ThrSer: 3.245 ± 0.084	3.515ThrThr: 3.515 ± 0.095	4.261ThrVal: 4.261 ± 0.106	0.437ThrTrp: 0.437 ± 0.028	2.208ThrTyr: 2.208 ± 0.062	0.001ThrXaa: 0.001 ± 0.001
Val	5.023ValAla: 5.023 ± 0.096	1.158ValCys: 1.158 ± 0.049	3.784ValAsp: 3.784 ± 0.067	4.34ValGlu: 4.34 ± 0.1	2.866ValPhe: 2.866 ± 0.069	4.032ValGly: 4.032 ± 0.088	1.117ValHis: 1.117 ± 0.033	5.389ValIle: 5.389 ± 0.107	4.864ValLys: 4.864 ± 0.089	6.288ValLeu: 6.288 ± 0.082	1.956ValMet: 1.956 ± 0.054	2.852ValAsn: 2.852 ± 0.071	2.183ValPro: 2.183 ± 0.062	2.08ValGln: 2.08 ± 0.053	2.557ValArg: 2.557 ± 0.07	4.854ValSer: 4.854 ± 0.094	4.413ValThr: 4.413 ± 0.097	4.989ValVal: 4.989 ± 0.104	0.559ValTrp: 0.559 ± 0.029	2.738ValTyr: 2.738 ± 0.063	0.0ValXaa: 0.0 ± 0.0
Trp	0.401TrpAla: 0.401 ± 0.025	0.155TrpCys: 0.155 ± 0.013	0.549TrpAsp: 0.549 ± 0.025	0.542TrpGlu: 0.542 ± 0.026	0.348TrpPhe: 0.348 ± 0.025	0.592TrpGly: 0.592 ± 0.03	0.171TrpHis: 0.171 ± 0.013	0.648TrpIle: 0.648 ± 0.03	0.77TrpLys: 0.77 ± 0.033	0.737TrpLeu: 0.737 ± 0.031	0.3TrpMet: 0.3 ± 0.021	0.504TrpAsn: 0.504 ± 0.026	0.15TrpPro: 0.15 ± 0.016	0.355TrpGln: 0.355 ± 0.029	0.274TrpArg: 0.274 ± 0.02	0.513TrpSer: 0.513 ± 0.029	0.354TrpThr: 0.354 ± 0.022	0.396TrpVal: 0.396 ± 0.024	0.098TrpTrp: 0.098 ± 0.012	0.38TrpTyr: 0.38 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.806TyrAla: 2.806 ± 0.064	0.666TyrCys: 0.666 ± 0.033	2.773TyrAsp: 2.773 ± 0.066	2.891TyrGlu: 2.891 ± 0.067	1.907TyrPhe: 1.907 ± 0.052	2.685TyrGly: 2.685 ± 0.064	1.053TyrHis: 1.053 ± 0.037	2.968TyrIle: 2.968 ± 0.064	2.438TyrLys: 2.438 ± 0.063	3.872TyrLeu: 3.872 ± 0.092	1.076TyrMet: 1.076 ± 0.038	1.838TyrAsn: 1.838 ± 0.053	1.393TyrPro: 1.393 ± 0.045	2.019TyrGln: 2.019 ± 0.056	2.168TyrArg: 2.168 ± 0.066	2.454TyrSer: 2.454 ± 0.062	2.409TyrThr: 2.409 ± 0.07	2.636TyrVal: 2.636 ± 0.052	0.337TyrTrp: 0.337 ± 0.024	1.997TyrTyr: 1.997 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.003XaaThr: 0.003 ± 0.002	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.021XaaXaa: 0.021 ± 0.008

Statistics based on 2209 proteins (717718 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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