Wenling crustacean virus 13
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KNB5|A0A1L3KNB5_9VIRU Putative glycoprotein OS=Wenling crustacean virus 13 OX=1923482 PE=4 SV=1
MM1 pKa = 7.48 HH2 pKa = 6.51 SHH4 pKa = 5.8 VSIVHH9 pKa = 6.49 GGHH12 pKa = 5.65 EE13 pKa = 4.48 AGRR16 pKa = 11.84 MTLLPNQCLDD26 pKa = 3.18 MHH28 pKa = 7.6 KK29 pKa = 11.05 NMAYY33 pKa = 8.68 QHH35 pKa = 6.56 HH36 pKa = 5.81 GHH38 pKa = 6.07 MVLLDD43 pKa = 4.0 GPNTITDD50 pKa = 3.39 ATITVAGSVDD60 pKa = 3.31 SSGWCEE66 pKa = 3.56 GSSFSHH72 pKa = 6.7 RR73 pKa = 11.84 GSSYY77 pKa = 8.49 TSVVVVDD84 pKa = 3.52 TLTIKK89 pKa = 10.53 VKK91 pKa = 10.65 QVDD94 pKa = 4.0 ALVQATSGEE103 pKa = 4.1 VHH105 pKa = 6.76 LPNGVSCLLGSKK117 pKa = 9.98 DD118 pKa = 4.33 CYY120 pKa = 10.45 HH121 pKa = 7.79 PIYY124 pKa = 10.75 GMAVIDD130 pKa = 3.66 EE131 pKa = 4.55 TNIGGCGDD139 pKa = 4.14 DD140 pKa = 3.31 SHH142 pKa = 7.68 EE143 pKa = 4.79 IIYY146 pKa = 9.15 EE147 pKa = 4.13 GPIEE151 pKa = 4.17 MVSMFGDD158 pKa = 3.2 SGEE161 pKa = 4.04 DD162 pKa = 2.94 EE163 pKa = 4.78 RR164 pKa = 11.84 YY165 pKa = 9.47 FVVEE169 pKa = 3.93 TPEE172 pKa = 3.96 TVFALKK178 pKa = 10.08 RR179 pKa = 11.84 GKK181 pKa = 10.07 KK182 pKa = 9.31 IQVCRR187 pKa = 11.84 EE188 pKa = 4.02 NAWSTEE194 pKa = 3.88 HH195 pKa = 6.96 PKK197 pKa = 10.84 LIVIPKK203 pKa = 8.78 PEE205 pKa = 3.92 WGFKK209 pKa = 6.94 FTKK212 pKa = 9.93 RR213 pKa = 11.84 ISGASSVHH221 pKa = 6.77 LIPYY225 pKa = 8.99 VNSKK229 pKa = 9.83 IQFLDD234 pKa = 3.53 LKK236 pKa = 10.56 VGQNMVRR243 pKa = 11.84 TKK245 pKa = 10.84 VEE247 pKa = 4.06 LLEE250 pKa = 4.34 QICEE254 pKa = 3.94 NSRR257 pKa = 11.84 KK258 pKa = 9.32 IVEE261 pKa = 4.15 NRR263 pKa = 11.84 LMLAKK268 pKa = 10.57 LFPHH272 pKa = 5.99 QVASLHH278 pKa = 5.21 YY279 pKa = 9.42 NKK281 pKa = 9.27 PGYY284 pKa = 8.53 MGRR287 pKa = 11.84 VSGEE291 pKa = 3.52 ALHH294 pKa = 7.13 IIKK297 pKa = 10.14 CKK299 pKa = 9.25 PVSVSGRR306 pKa = 11.84 KK307 pKa = 9.32 AEE309 pKa = 4.07 GCYY312 pKa = 9.93 QGIPVTHH319 pKa = 6.86 KK320 pKa = 10.28 NEE322 pKa = 3.93 SWFLLPITRR331 pKa = 11.84 ILSRR335 pKa = 11.84 NAVEE339 pKa = 4.65 TTCSRR344 pKa = 11.84 RR345 pKa = 11.84 LPNVYY350 pKa = 10.36 LFGDD354 pKa = 3.94 SWWEE358 pKa = 3.7 LGPEE362 pKa = 4.18 PRR364 pKa = 11.84 PAQPPKK370 pKa = 10.17 TMTVGSLLPQWGRR383 pKa = 11.84 TRR385 pKa = 11.84 TSPLGFGGLYY395 pKa = 9.94 PYY397 pKa = 10.41 RR398 pKa = 11.84 DD399 pKa = 3.2 MMAYY403 pKa = 9.04 EE404 pKa = 3.93 QALFGPVVTDD414 pKa = 2.94 TGIAIMTRR422 pKa = 11.84 KK423 pKa = 6.05 MTGMASHH430 pKa = 7.86 DD431 pKa = 3.73 EE432 pKa = 4.53 TFSSTKK438 pKa = 10.65 LFDD441 pKa = 3.61 QDD443 pKa = 5.27 DD444 pKa = 3.83 MGSFQDD450 pKa = 5.66 SLIEE454 pKa = 4.48 HH455 pKa = 6.3 NWGPLAWMGEE465 pKa = 3.88 ILAEE469 pKa = 4.15 GAAVIVCIVLILLLLKK485 pKa = 10.68 LVVRR489 pKa = 11.84 VYY491 pKa = 11.0 KK492 pKa = 10.63 LWRR495 pKa = 11.84 VSGAGPWLLWSILSVFTEE513 pKa = 5.08 TYY515 pKa = 9.17 TAIKK519 pKa = 10.28 FITNLDD525 pKa = 4.34 KK526 pKa = 10.93 IPCSACHH533 pKa = 6.66 PCTCHH538 pKa = 6.22 TRR540 pKa = 11.84 TDD542 pKa = 3.49 STQEE546 pKa = 3.4 IDD548 pKa = 3.69 SEE550 pKa = 4.2 NGRR553 pKa = 11.84 LYY555 pKa = 10.38 PVFSHH560 pKa = 7.33 ADD562 pKa = 3.06 IAVTNAEE569 pKa = 4.28 VAA571 pKa = 3.53
Molecular weight: 63.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.04
IPC2_protein 6.02
IPC_protein 6.109
Toseland 6.453
ProMoST 6.532
Dawson 6.453
Bjellqvist 6.453
Wikipedia 6.478
Rodwell 6.453
Grimsley 6.59
Solomon 6.465
Lehninger 6.453
Nozaki 6.751
DTASelect 6.927
Thurlkill 6.956
EMBOSS 6.912
Sillero 6.854
Patrickios 3.948
IPC_peptide 6.478
IPC2_peptide 6.795
IPC2.peptide.svr19 6.732
Protein with the highest isoelectric point:
>tr|A0A1L3KN74|A0A1L3KN74_9VIRU Large structural protein OS=Wenling crustacean virus 13 OX=1923482 PE=4 SV=1
MM1 pKa = 7.96 KK2 pKa = 10.15 IPQILLKK9 pKa = 10.03 HH10 pKa = 5.99 PPVLYY15 pKa = 10.29 VVVVMAQHH23 pKa = 4.74 VHH25 pKa = 5.87 LVNGLLDD32 pKa = 3.51 KK33 pKa = 11.29 AGFWRR38 pKa = 11.84 EE39 pKa = 3.71 VLKK42 pKa = 11.17 QEE44 pKa = 4.2 DD45 pKa = 5.1 PYY47 pKa = 11.34 MKK49 pKa = 10.45 LCLRR53 pKa = 11.84 HH54 pKa = 6.09 CNARR58 pKa = 11.84 PWVAPDD64 pKa = 5.22 GIRR67 pKa = 11.84 KK68 pKa = 8.45 WGRR71 pKa = 11.84 AMEE74 pKa = 4.38 VAWLLSTRR82 pKa = 11.84 IVATLRR88 pKa = 11.84 VTTPHH93 pKa = 6.9 SILVWTMMEE102 pKa = 3.85 TLAPQVHH109 pKa = 6.91 DD110 pKa = 3.46 EE111 pKa = 4.27 RR112 pKa = 11.84 YY113 pKa = 9.51 IPRR116 pKa = 11.84 LEE118 pKa = 4.1 ANALIVRR125 pKa = 11.84 SVYY128 pKa = 10.61 NDD130 pKa = 3.17 SSFQSLVSNYY140 pKa = 8.97 TDD142 pKa = 3.81 ANLAQDD148 pKa = 4.27 LHH150 pKa = 7.35 DD151 pKa = 5.31 LSQDD155 pKa = 3.34 PDD157 pKa = 3.74 FEE159 pKa = 5.3 RR160 pKa = 11.84 PANDD164 pKa = 3.86 DD165 pKa = 3.72 GVPTTIAIQFSAVANKK181 pKa = 10.1 SAEE184 pKa = 3.95 GAMAILWVLAKK195 pKa = 10.43 RR196 pKa = 11.84 SVNLHH201 pKa = 5.06 TRR203 pKa = 11.84 PAQVLVHH210 pKa = 5.94 TLCALAKK217 pKa = 10.15 QGNATQAFVEE227 pKa = 5.05 KK228 pKa = 9.19 ITQGFVTDD236 pKa = 3.36 TGKK239 pKa = 9.74 PLNLDD244 pKa = 3.3 AEE246 pKa = 4.95 GIEE249 pKa = 4.28 ACWRR253 pKa = 11.84 LIVHH257 pKa = 6.67 HH258 pKa = 7.02 VDD260 pKa = 3.5 DD261 pKa = 5.35 SNVEE265 pKa = 4.31 AIVSDD270 pKa = 3.78 WLAKK274 pKa = 10.29 LPHH277 pKa = 6.09 EE278 pKa = 4.43 ALRR281 pKa = 11.84 LRR283 pKa = 11.84 ITLQQIPGEE292 pKa = 4.24 GLTCITSIIKK302 pKa = 10.36 AIQDD306 pKa = 3.38 FPGFNWTWVYY316 pKa = 11.5 KK317 pKa = 10.25 NFPGEE322 pKa = 3.72 MTAAGTASIAITNRR336 pKa = 11.84 GFYY339 pKa = 10.23 GYY341 pKa = 10.55 KK342 pKa = 9.73 KK343 pKa = 10.44 DD344 pKa = 3.76 LSLVKK349 pKa = 10.1 AANFRR354 pKa = 11.84 TVGYY358 pKa = 9.74 IAQQLLVRR366 pKa = 11.84 AGGEE370 pKa = 4.33 GPLRR374 pKa = 11.84 AAKK377 pKa = 10.43 CFTRR381 pKa = 11.84 TPKK384 pKa = 10.61 LKK386 pKa = 10.76 ALIDD390 pKa = 3.6 QMIDD394 pKa = 3.32 DD395 pKa = 5.28 FIINAVTIPVNPGDD409 pKa = 4.27 EE410 pKa = 4.34 VPAGIWASIMGYY422 pKa = 8.64 SVPPAPQPVLMAGPQPGGGHH442 pKa = 6.9 GGGQLPPGGPGPGAPPAPPQPQAGPNVQGGNRR474 pKa = 11.84 PP475 pKa = 3.35
Molecular weight: 51.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.956
IPC2_protein 7.249
IPC_protein 7.263
Toseland 7.117
ProMoST 7.863
Dawson 7.907
Bjellqvist 8.141
Wikipedia 7.834
Rodwell 7.907
Grimsley 7.205
Solomon 7.995
Lehninger 8.009
Nozaki 8.39
DTASelect 8.039
Thurlkill 8.083
EMBOSS 8.156
Sillero 8.361
Patrickios 4.482
IPC_peptide 7.995
IPC2_peptide 7.395
IPC2.peptide.svr19 7.336
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3224
475
2178
1074.7
121.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.669 ± 1.071
2.233 ± 0.272
4.932 ± 0.195
6.234 ± 0.84
3.226 ± 0.153
5.552 ± 1.264
3.35 ± 0.239
6.328 ± 0.009
4.901 ± 0.202
10.205 ± 0.682
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.294
3.66 ± 0.192
5.18 ± 0.958
3.412 ± 0.457
5.924 ± 0.783
6.886 ± 0.859
5.955 ± 0.23
7.444 ± 0.447
2.171 ± 0.041
3.319 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here