Odonata-associated circular virus-14
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UI10|A0A0B4UI10_9VIRU ATP-dependent helicase Rep OS=Odonata-associated circular virus-14 OX=1592114 PE=3 SV=1
MM1 pKa = 7.19 SQSQQSRR8 pKa = 11.84 RR9 pKa = 11.84 WFITINNYY17 pKa = 8.64 TSADD21 pKa = 3.06 IDD23 pKa = 3.47 ALRR26 pKa = 11.84 EE27 pKa = 3.98 IEE29 pKa = 4.32 CNYY32 pKa = 9.88 IVACTEE38 pKa = 3.68 IAPTTGTPHH47 pKa = 4.61 VHH49 pKa = 6.51 ALVIFNTNKK58 pKa = 9.99 RR59 pKa = 11.84 FFAVKK64 pKa = 10.07 RR65 pKa = 11.84 LVARR69 pKa = 11.84 ADD71 pKa = 3.27 IEE73 pKa = 4.41 IVKK76 pKa = 8.8 GTFNQAYY83 pKa = 10.18 DD84 pKa = 3.78 YY85 pKa = 8.79 VTKK88 pKa = 10.44 DD89 pKa = 2.97 GRR91 pKa = 11.84 IILEE95 pKa = 4.34 EE96 pKa = 4.05 GDD98 pKa = 3.75 KK99 pKa = 11.01 PKK101 pKa = 10.69 EE102 pKa = 3.91 KK103 pKa = 10.82 HH104 pKa = 6.0 NVEE107 pKa = 4.39 CTFKK111 pKa = 11.38 AMVQAAKK118 pKa = 10.57 DD119 pKa = 3.53 GTIDD123 pKa = 4.2 KK124 pKa = 9.78 EE125 pKa = 4.0 CLMYY129 pKa = 10.54 CRR131 pKa = 11.84 YY132 pKa = 10.27 EE133 pKa = 4.01 KK134 pKa = 10.64 FFSRR138 pKa = 11.84 FEE140 pKa = 3.92 KK141 pKa = 10.7 RR142 pKa = 11.84 EE143 pKa = 3.88 EE144 pKa = 3.76 FCYY147 pKa = 10.77 DD148 pKa = 3.72 GEE150 pKa = 4.45 LSCKK154 pKa = 9.73 NAWIYY159 pKa = 10.28 GPPGTGKK166 pKa = 9.84 SRR168 pKa = 11.84 LVRR171 pKa = 11.84 EE172 pKa = 4.33 YY173 pKa = 11.01 ARR175 pKa = 11.84 SRR177 pKa = 11.84 GYY179 pKa = 10.48 RR180 pKa = 11.84 IYY182 pKa = 11.04 EE183 pKa = 3.94 KK184 pKa = 10.77 LSNKK188 pKa = 8.26 WWDD191 pKa = 3.5 NYY193 pKa = 11.11 DD194 pKa = 3.52 GEE196 pKa = 4.7 EE197 pKa = 4.18 VVLMEE202 pKa = 5.5 DD203 pKa = 4.71 LDD205 pKa = 4.22 PKK207 pKa = 9.86 VCEE210 pKa = 4.11 VLIHH214 pKa = 6.91 HH215 pKa = 6.87 IKK217 pKa = 10.4 LWADD221 pKa = 4.12 RR222 pKa = 11.84 YY223 pKa = 9.79 PFRR226 pKa = 11.84 AEE228 pKa = 3.72 VKK230 pKa = 10.16 GGSKK234 pKa = 10.4 RR235 pKa = 11.84 LLPKK239 pKa = 10.04 FQLIVTSHH247 pKa = 6.21 YY248 pKa = 11.0 SLSEE252 pKa = 4.26 CFTGPDD258 pKa = 3.12 GSAICRR264 pKa = 11.84 RR265 pKa = 11.84 FDD267 pKa = 3.25 EE268 pKa = 4.56 WEE270 pKa = 4.04 MNN272 pKa = 3.57
Molecular weight: 31.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.593
IPC2_protein 6.751
IPC_protein 6.766
Toseland 6.561
ProMoST 7.234
Dawson 7.38
Bjellqvist 7.512
Wikipedia 7.307
Rodwell 7.395
Grimsley 6.62
Solomon 7.424
Lehninger 7.454
Nozaki 7.834
DTASelect 7.585
Thurlkill 7.629
EMBOSS 7.644
Sillero 7.878
Patrickios 4.088
IPC_peptide 7.424
IPC2_peptide 7.132
IPC2.peptide.svr19 7.065
Protein with the highest isoelectric point:
>tr|A0A0B4UI10|A0A0B4UI10_9VIRU ATP-dependent helicase Rep OS=Odonata-associated circular virus-14 OX=1592114 PE=3 SV=1
MM1 pKa = 7.96 PSRR4 pKa = 11.84 PTRR7 pKa = 11.84 VSRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 SNKK15 pKa = 8.2 KK16 pKa = 8.47 WSPVLIQEE24 pKa = 4.42 SVSYY28 pKa = 10.74 SLGGTSVKK36 pKa = 10.36 LSSVPLCTNSNNTSLAPTATIIKK59 pKa = 9.65 AGNFKK64 pKa = 10.87 VVVDD68 pKa = 4.29 VNISDD73 pKa = 3.93 SFTGSGRR80 pKa = 11.84 MYY82 pKa = 11.43 VMFVPQGYY90 pKa = 9.67 DD91 pKa = 3.02 LANATSAFPGQHH103 pKa = 6.78 PEE105 pKa = 4.81 WIMCWRR111 pKa = 11.84 GFEE114 pKa = 4.35 PGHH117 pKa = 6.48 NGLQAVSMQSKK128 pKa = 10.22 LKK130 pKa = 10.68 RR131 pKa = 11.84 NLNSGDD137 pKa = 3.69 QIVLVIVANNLSGVASTVSLTVSCSYY163 pKa = 10.44 VCCANYY169 pKa = 10.44 NDD171 pKa = 4.62 SFFF174 pKa = 5.42
Molecular weight: 18.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.847
IPC2_protein 8.682
IPC_protein 8.668
Toseland 9.048
ProMoST 9.048
Dawson 9.414
Bjellqvist 9.399
Wikipedia 9.56
Rodwell 9.545
Grimsley 9.37
Solomon 9.545
Lehninger 9.516
Nozaki 9.56
DTASelect 9.253
Thurlkill 9.326
EMBOSS 9.56
Sillero 9.545
Patrickios 4.507
IPC_peptide 9.531
IPC2_peptide 8.507
IPC2.peptide.svr19 7.801
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
446
174
272
223.0
25.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.054 ± 0.169
3.363 ± 0.27
4.709 ± 1.013
6.054 ± 2.391
4.709 ± 0.379
6.054 ± 0.465
1.794 ± 0.356
5.605 ± 0.874
6.278 ± 1.245
6.278 ± 0.342
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.242 ± 0.349
5.605 ± 1.348
4.036 ± 0.628
2.691 ± 0.418
6.278 ± 0.928
8.744 ± 3.106
5.381 ± 0.202
7.848 ± 1.697
2.018 ± 0.162
4.26 ± 0.766
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here