Oryza punctata (Red rice)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E0JYC5|A0A0E0JYC5_ORYPU Uncharacterized protein OS=Oryza punctata OX=4537 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 6.64CDD4 pKa = 3.34MDD6 pKa = 4.35DD7 pKa = 3.72LRR9 pKa = 11.84HH10 pKa = 6.28RR11 pKa = 11.84LLTVEE16 pKa = 3.99THH18 pKa = 6.74CFDD21 pKa = 3.46HH22 pKa = 6.99HH23 pKa = 7.45HH24 pKa = 7.46DD25 pKa = 3.84GCVVDD30 pKa = 3.82VAGANVAEE38 pKa = 4.23EE39 pKa = 4.31MVLSKK44 pKa = 10.81CVADD48 pKa = 3.77VDD50 pKa = 4.26LTGGKK55 pKa = 9.89DD56 pKa = 3.58LDD58 pKa = 4.03SGLCVLLDD66 pKa = 3.83EE67 pKa = 5.47KK68 pKa = 11.05VCQYY72 pKa = 10.45MIDD75 pKa = 4.3CIVEE79 pKa = 3.88VGVAEE84 pKa = 4.74VYY86 pKa = 10.75AEE88 pKa = 4.14EE89 pKa = 5.4PIVIDD94 pKa = 4.65VSEE97 pKa = 4.48EE98 pKa = 4.03DD99 pKa = 4.95DD100 pKa = 4.86DD101 pKa = 5.96GSDD104 pKa = 4.45YY105 pKa = 10.9EE106 pKa = 5.41LEE108 pKa = 4.19MAKK111 pKa = 10.54DD112 pKa = 3.56HH113 pKa = 6.86ANEE116 pKa = 4.42DD117 pKa = 3.93SEE119 pKa = 4.71EE120 pKa = 4.2DD121 pKa = 3.65EE122 pKa = 4.45PVNGNGDD129 pKa = 3.8EE130 pKa = 4.11PVKK133 pKa = 11.23GNGNEE138 pKa = 3.71EE139 pKa = 4.09DD140 pKa = 3.82EE141 pKa = 4.44PVNGNEE147 pKa = 5.31DD148 pKa = 3.88EE149 pKa = 4.5PLHH152 pKa = 6.21

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E0MEI5|A0A0E0MEI5_ORYPU Uncharacterized protein OS=Oryza punctata OX=4537 PE=4 SV=1
MM1 pKa = 7.83PSLTTGRR8 pKa = 11.84SPWRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 10.16RR16 pKa = 11.84GTPAVGTTTARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84TPTSLRR36 pKa = 11.84ALWRR40 pKa = 11.84RR41 pKa = 11.84IVPRR45 pKa = 11.84TSTTTAPRR53 pKa = 11.84VRR55 pKa = 11.84TRR57 pKa = 11.84KK58 pKa = 9.6PGLLSRR64 pKa = 11.84AFRR67 pKa = 11.84VLSCGGGRR75 pKa = 11.84RR76 pKa = 11.84SRR78 pKa = 11.84AARR81 pKa = 11.84RR82 pKa = 11.84WW83 pKa = 3.29

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31311

7120

38431

16585171

7

5284

431.6

47.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.955 ± 0.017

1.904 ± 0.007

5.437 ± 0.009

6.05 ± 0.015

3.666 ± 0.009

7.272 ± 0.015

2.51 ± 0.005

4.54 ± 0.01

4.988 ± 0.012

9.509 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.005

3.644 ± 0.009

5.489 ± 0.013

3.455 ± 0.01

6.213 ± 0.01

8.383 ± 0.015

4.858 ± 0.007

6.776 ± 0.009

1.3 ± 0.004

2.602 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski