Salmonella phage SEN4
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4R507|A0A0M4R507_9CAUD Late control D protein OS=Salmonella phage SEN4 OX=1647465 GN=SEN4_28 PE=4 SV=1
MM1 pKa = 7.64 SGTIDD6 pKa = 4.84 LSQLPPPVVVEE17 pKa = 4.06 PLDD20 pKa = 3.89 FEE22 pKa = 4.7 TLFAQRR28 pKa = 11.84 KK29 pKa = 6.95 AAFIAMYY36 pKa = 10.23 PEE38 pKa = 4.92 DD39 pKa = 4.31 EE40 pKa = 4.28 QEE42 pKa = 4.23 EE43 pKa = 4.27 IARR46 pKa = 11.84 TLEE49 pKa = 4.3 LEE51 pKa = 4.2 SEE53 pKa = 4.87 PITMLLEE60 pKa = 4.14 EE61 pKa = 4.03 NCYY64 pKa = 10.44 RR65 pKa = 11.84 EE66 pKa = 4.01 LLLRR70 pKa = 11.84 QRR72 pKa = 11.84 VNEE75 pKa = 3.92 AARR78 pKa = 11.84 AVMLAYY84 pKa = 9.8 STDD87 pKa = 3.45 SDD89 pKa = 4.56 LDD91 pKa = 3.66 NLAVNFNVEE100 pKa = 3.66 RR101 pKa = 11.84 LTIQEE106 pKa = 4.41 EE107 pKa = 4.42 DD108 pKa = 4.01 DD109 pKa = 4.2 SVTPPIEE116 pKa = 4.01 AVVEE120 pKa = 4.06 SDD122 pKa = 2.72 ADD124 pKa = 3.56 LRR126 pKa = 11.84 TRR128 pKa = 11.84 TQQAFEE134 pKa = 4.23 GLSVAGPTAAYY145 pKa = 8.17 EE146 pKa = 4.04 FWGRR150 pKa = 11.84 SADD153 pKa = 3.49 GRR155 pKa = 11.84 VADD158 pKa = 4.7 ISAVSPTPACVTISVLSRR176 pKa = 11.84 EE177 pKa = 4.37 GDD179 pKa = 3.47 GTASDD184 pKa = 4.66 DD185 pKa = 3.98 LLSVVAAALNDD196 pKa = 3.71 EE197 pKa = 4.58 EE198 pKa = 4.51 VRR200 pKa = 11.84 PVADD204 pKa = 3.01 RR205 pKa = 11.84 VTVQSADD212 pKa = 3.06 IVPYY216 pKa = 10.65 QIDD219 pKa = 3.74 ATLYY223 pKa = 9.62 IYY225 pKa = 9.19 PGPEE229 pKa = 3.79 AEE231 pKa = 4.54 PVRR234 pKa = 11.84 QASEE238 pKa = 4.05 QQLQAYY244 pKa = 6.87 ITAQNRR250 pKa = 11.84 LGRR253 pKa = 11.84 DD254 pKa = 2.99 IRR256 pKa = 11.84 ISAIYY261 pKa = 9.65 AALHH265 pKa = 5.3 VEE267 pKa = 4.81 GVQRR271 pKa = 11.84 VEE273 pKa = 3.98 LAQPVADD280 pKa = 4.37 IVLSDD285 pKa = 3.81 YY286 pKa = 10.63 QASHH290 pKa = 5.6 CTEE293 pKa = 3.71 YY294 pKa = 10.62 TITVGGYY301 pKa = 10.39 DD302 pKa = 3.42 EE303 pKa = 4.8
Molecular weight: 33.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.146
IPC2_protein 4.05
IPC_protein 4.012
Toseland 3.834
ProMoST 4.126
Dawson 3.973
Bjellqvist 4.151
Wikipedia 3.859
Rodwell 3.846
Grimsley 3.732
Solomon 3.973
Lehninger 3.923
Nozaki 4.088
DTASelect 4.253
Thurlkill 3.846
EMBOSS 3.872
Sillero 4.126
Patrickios 3.3
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.05
Protein with the highest isoelectric point:
>tr|A0A0M5M1H9|A0A0M5M1H9_9CAUD Integrase OS=Salmonella phage SEN4 OX=1647465 GN=SEN4_31 PE=3 SV=1
MM1 pKa = 7.74 TGQQTDD7 pKa = 3.79 PLFQQLDD14 pKa = 3.38 DD15 pKa = 3.49 WLARR19 pKa = 11.84 VAAQLSPGHH28 pKa = 5.97 RR29 pKa = 11.84 RR30 pKa = 11.84 KK31 pKa = 8.87 LTRR34 pKa = 11.84 DD35 pKa = 3.19 VAIGLRR41 pKa = 11.84 KK42 pKa = 9.46 RR43 pKa = 11.84 QQKK46 pKa = 10.28 RR47 pKa = 11.84 IASQKK52 pKa = 8.7 NPSGEE57 pKa = 4.15 SYY59 pKa = 9.16 QARR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 KK65 pKa = 9.45 ILRR68 pKa = 11.84 TQGGIKK74 pKa = 9.63 FIWNDD79 pKa = 3.07 EE80 pKa = 3.84 ARR82 pKa = 11.84 EE83 pKa = 3.95 LRR85 pKa = 11.84 NWRR88 pKa = 11.84 TTGRR92 pKa = 11.84 GEE94 pKa = 3.67 HH95 pKa = 6.29 RR96 pKa = 11.84 AITGYY101 pKa = 10.81 DD102 pKa = 3.18 VDD104 pKa = 4.59 RR105 pKa = 11.84 GALRR109 pKa = 11.84 TFYY112 pKa = 10.87 KK113 pKa = 10.27 RR114 pKa = 11.84 DD115 pKa = 2.95 IQRR118 pKa = 11.84 YY119 pKa = 8.88 IEE121 pKa = 4.19 INLNQSKK128 pKa = 9.4 QNRR131 pKa = 11.84 TRR133 pKa = 11.84 KK134 pKa = 10.36 DD135 pKa = 3.07 PMFRR139 pKa = 11.84 KK140 pKa = 9.77 LRR142 pKa = 11.84 TARR145 pKa = 11.84 FLKK148 pKa = 10.65 AYY150 pKa = 6.05 GTSGIAVVGFQGHH163 pKa = 4.6 TAEE166 pKa = 4.51 IASVHH171 pKa = 5.79 QYY173 pKa = 11.53 GEE175 pKa = 3.9 VDD177 pKa = 3.46 NVVPGARR184 pKa = 11.84 ARR186 pKa = 11.84 YY187 pKa = 7.83 PVRR190 pKa = 11.84 EE191 pKa = 4.09 LLGMTEE197 pKa = 5.85 GDD199 pKa = 4.56 LDD201 pKa = 3.8 WLADD205 pKa = 3.66 TVVAFMQEE213 pKa = 3.51 II214 pKa = 3.73
Molecular weight: 24.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.721
IPC_protein 10.643
Toseland 10.716
ProMoST 10.467
Dawson 10.818
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.965
Grimsley 10.877
Solomon 10.95
Lehninger 10.906
Nozaki 10.687
DTASelect 10.555
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.657
IPC_peptide 10.95
IPC2_peptide 9.385
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
10358
38
974
220.4
24.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.34 ± 0.59
1.197 ± 0.191
6.15 ± 0.295
5.793 ± 0.31
3.437 ± 0.318
7.289 ± 0.296
1.757 ± 0.189
5.252 ± 0.209
5.223 ± 0.326
8.37 ± 0.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.365 ± 0.159
3.92 ± 0.199
4.518 ± 0.207
4.364 ± 0.381
6.343 ± 0.507
6.179 ± 0.323
6.246 ± 0.401
6.488 ± 0.226
1.574 ± 0.163
3.196 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here