Marixanthomonas sp. HN-E44
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3068 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U0HX65|A0A2U0HX65_9FLAO Uncharacterized protein OS=Marixanthomonas sp. HN-E44 OX=2174845 GN=DDV96_13620 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 9.05 KK3 pKa = 8.31 TLLLLITLCCGFCYY17 pKa = 9.6 GQNYY21 pKa = 8.91 NVSIYY26 pKa = 8.92 QQIADD31 pKa = 3.76 NKK33 pKa = 9.95 GGFNAAIDD41 pKa = 3.59 NADD44 pKa = 2.93 WFGYY48 pKa = 8.77 SVEE51 pKa = 5.08 SIGDD55 pKa = 3.77 LNGDD59 pKa = 3.77 GIEE62 pKa = 4.13 DD63 pKa = 3.84 VVVGSLKK70 pKa = 10.92 DD71 pKa = 3.48 DD72 pKa = 4.08 DD73 pKa = 5.2 GGFNRR78 pKa = 11.84 GALYY82 pKa = 10.94 VLFLNRR88 pKa = 11.84 DD89 pKa = 3.77 GEE91 pKa = 4.17 VDD93 pKa = 3.76 SYY95 pKa = 11.81 QKK97 pKa = 10.67 ISDD100 pKa = 4.03 TEE102 pKa = 4.25 GGFQGVLDD110 pKa = 4.27 DD111 pKa = 3.86 WDD113 pKa = 3.82 IFGSSISYY121 pKa = 10.56 LGDD124 pKa = 3.18 INNDD128 pKa = 2.73 GFTEE132 pKa = 3.95 IGVGAEE138 pKa = 3.81 YY139 pKa = 10.81 DD140 pKa = 3.37 GDD142 pKa = 3.45 GGYY145 pKa = 8.17 WHH147 pKa = 6.88 GAVWILSLDD156 pKa = 3.61 SNGIVQSYY164 pKa = 10.66 SKK166 pKa = 10.68 ISDD169 pKa = 3.68 TEE171 pKa = 4.28 GGFLAPLGDD180 pKa = 3.62 EE181 pKa = 5.02 DD182 pKa = 5.76 VFGTDD187 pKa = 2.94 IASLGDD193 pKa = 3.71 LNGDD197 pKa = 3.58 GFGDD201 pKa = 3.38 IVVSARR207 pKa = 11.84 RR208 pKa = 11.84 DD209 pKa = 3.38 PDD211 pKa = 3.64 GGSDD215 pKa = 3.18 RR216 pKa = 11.84 GAVYY220 pKa = 10.44 ILFLNNDD227 pKa = 3.75 LSVNNYY233 pKa = 8.34 QKK235 pKa = 10.52 ISQTQGNFQGYY246 pKa = 9.68 LDD248 pKa = 4.34 PGDD251 pKa = 3.93 YY252 pKa = 10.8 FGGAVANIGDD262 pKa = 3.89 IDD264 pKa = 4.88 LDD266 pKa = 4.09 GVVDD270 pKa = 3.9 IAVGAYY276 pKa = 9.72 RR277 pKa = 11.84 DD278 pKa = 3.93 DD279 pKa = 5.58 DD280 pKa = 4.26 GGIDD284 pKa = 3.38 QGAVYY289 pKa = 10.81 VLFLNSDD296 pKa = 3.92 GSVKK300 pKa = 10.39 DD301 pKa = 3.59 YY302 pKa = 11.53 QKK304 pKa = 10.84 ISDD307 pKa = 3.98 VYY309 pKa = 11.01 GGLEE313 pKa = 3.9 TTFNNGMFFGTSIDD327 pKa = 3.48 LAYY330 pKa = 10.48 DD331 pKa = 3.23 INDD334 pKa = 3.46 NGLTEE339 pKa = 4.83 IIVGAKK345 pKa = 10.16 GYY347 pKa = 9.44 WNEE350 pKa = 3.93 EE351 pKa = 3.68 NSEE354 pKa = 3.72 IGAFYY359 pKa = 10.51 ILEE362 pKa = 4.49 LNNNGTVNNSVRR374 pKa = 11.84 YY375 pKa = 7.54 TKK377 pKa = 10.89 GEE379 pKa = 3.85 QFFDD383 pKa = 3.67 GDD385 pKa = 4.23 VVPEE389 pKa = 4.06 SSFGFSVSYY398 pKa = 10.93 NRR400 pKa = 11.84 GLSTNDD406 pKa = 3.57 AIVIGAHH413 pKa = 4.59 TTGDD417 pKa = 3.07 INGFEE422 pKa = 4.44 NGSLWILQLGKK433 pKa = 10.05 ILSIDD438 pKa = 4.7 DD439 pKa = 3.21 IDD441 pKa = 4.55 GNNEE445 pKa = 3.85 QILVYY450 pKa = 9.93 PNPTNNSFSLHH461 pKa = 6.62 EE462 pKa = 4.81 IDD464 pKa = 6.35 DD465 pKa = 3.58 ILMIQIFDD473 pKa = 3.82 SQGKK477 pKa = 9.48 EE478 pKa = 3.49 IISFKK483 pKa = 10.53 DD484 pKa = 2.98 IKK486 pKa = 11.2 SNNFDD491 pKa = 3.4 VSFLPVGVYY500 pKa = 10.18 VILIKK505 pKa = 11.01 SNNEE509 pKa = 4.18 DD510 pKa = 2.91 ISSYY514 pKa = 11.46 KK515 pKa = 10.35 LIKK518 pKa = 10.2 NN519 pKa = 3.64
Molecular weight: 56.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.49
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.401
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.706
Sillero 3.859
Patrickios 1.163
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A2U0I0V5|A0A2U0I0V5_9FLAO DUF4168 domain-containing protein OS=Marixanthomonas sp. HN-E44 OX=2174845 GN=DDV96_08880 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.18 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.17 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.02 GRR39 pKa = 11.84 KK40 pKa = 8.12 KK41 pKa = 10.62 LSVSSEE47 pKa = 4.03 TRR49 pKa = 11.84 HH50 pKa = 5.96 KK51 pKa = 10.68 KK52 pKa = 9.8
Molecular weight: 6.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3068
0
3068
1006229
24
4048
328.0
36.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.701 ± 0.048
0.726 ± 0.018
5.605 ± 0.039
6.92 ± 0.045
5.107 ± 0.038
6.47 ± 0.051
1.797 ± 0.021
7.321 ± 0.045
7.775 ± 0.063
9.221 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.19 ± 0.028
5.882 ± 0.046
3.555 ± 0.024
3.52 ± 0.027
3.522 ± 0.037
6.109 ± 0.033
6.143 ± 0.067
6.434 ± 0.037
1.01 ± 0.017
3.992 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here