Podoviridae sp. ctQNx1
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W9U1|A0A5Q2W9U1_9CAUD Uncharacterized protein OS=Podoviridae sp. ctQNx1 OX=2656711 PE=4 SV=1
MM1 pKa = 8.13 VYY3 pKa = 10.01 EE4 pKa = 4.12 IDD6 pKa = 3.71 GGAVLEE12 pKa = 4.27 NEE14 pKa = 4.55 EE15 pKa = 4.17 YY16 pKa = 10.76 TLVGNDD22 pKa = 4.29 AEE24 pKa = 4.32 NAMDD28 pKa = 4.53 DD29 pKa = 4.24 RR30 pKa = 11.84 LTITHH35 pKa = 7.08 KK36 pKa = 10.98 ASGDD40 pKa = 3.95 SVTLTGLANMTMRR53 pKa = 11.84 HH54 pKa = 4.95 VLAEE58 pKa = 3.69 HH59 pKa = 6.48 GMRR62 pKa = 11.84 VIDD65 pKa = 5.06 LYY67 pKa = 10.73 FEE69 pKa = 3.87 RR70 pKa = 11.84 TAFFTLVNNTPGSTFIPASLSGSAVSS96 pKa = 3.93
Molecular weight: 10.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.466
IPC2_protein 4.507
IPC_protein 4.368
Toseland 4.215
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.228
Rodwell 4.215
Grimsley 4.139
Solomon 4.329
Lehninger 4.279
Nozaki 4.457
DTASelect 4.609
Thurlkill 4.24
EMBOSS 4.24
Sillero 4.495
Patrickios 3.63
IPC_peptide 4.329
IPC2_peptide 4.482
IPC2.peptide.svr19 4.409
Protein with the highest isoelectric point:
>tr|A0A5Q2W6B1|A0A5Q2W6B1_9CAUD Uncharacterized protein OS=Podoviridae sp. ctQNx1 OX=2656711 PE=4 SV=1
MM1 pKa = 7.41 LKK3 pKa = 9.88 VRR5 pKa = 11.84 IWFRR9 pKa = 11.84 VILPATATSRR19 pKa = 11.84 GAPRR23 pKa = 11.84 SGHH26 pKa = 6.07 PGVHH30 pKa = 4.93 IVVNARR36 pKa = 11.84 VHH38 pKa = 5.63 KK39 pKa = 10.7
Molecular weight: 4.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.281
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
11807
29
752
161.7
18.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.672 ± 0.537
1.609 ± 0.215
4.853 ± 0.28
6.488 ± 0.439
3.481 ± 0.242
7.275 ± 0.328
2.312 ± 0.228
5.717 ± 0.178
5.471 ± 0.36
7.758 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.159 ± 0.166
4.743 ± 0.279
4.226 ± 0.217
4.955 ± 0.56
5.793 ± 0.295
5.742 ± 0.32
5.632 ± 0.312
6.267 ± 0.414
1.448 ± 0.151
3.396 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here