Mycobacterium phage Omega (Mycobacteriophage Omega)
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q853V6|Q853V6_BPMOM Uncharacterized protein OS=Mycobacterium phage Omega OX=205879 GN=211 PE=4 SV=1
MM1 pKa = 7.45 SATADD6 pKa = 3.69 TLVSAFADD14 pKa = 3.47 LGEE17 pKa = 4.64 PLDD20 pKa = 5.63 DD21 pKa = 4.35 EE22 pKa = 5.07 ANCYY26 pKa = 10.75 LEE28 pKa = 4.47 VDD30 pKa = 3.71 TEE32 pKa = 4.05 EE33 pKa = 4.1 RR34 pKa = 11.84 QAIIVWVEE42 pKa = 3.24 PLAFHH47 pKa = 7.63 AEE49 pKa = 4.41 FNGPRR54 pKa = 11.84 QYY56 pKa = 11.42 VATSSYY62 pKa = 10.59 FVDD65 pKa = 4.42 KK66 pKa = 11.13 AMPDD70 pKa = 2.96 HH71 pKa = 6.82 WSAHH75 pKa = 4.34 VDD77 pKa = 2.93 ADD79 pKa = 3.42 GWLTRR84 pKa = 11.84 AVADD88 pKa = 4.69 AIVARR93 pKa = 11.84 LQGCIGARR101 pKa = 11.84 GEE103 pKa = 4.29 VYY105 pKa = 10.02 EE106 pKa = 4.57 LEE108 pKa = 4.42 YY109 pKa = 11.32 GGDD112 pKa = 3.55 EE113 pKa = 4.04 PNITFEE119 pKa = 4.3 IVTSYY124 pKa = 11.56 EE125 pKa = 3.87 DD126 pKa = 4.98 GEE128 pKa = 4.77 TFDD131 pKa = 3.78 HH132 pKa = 6.5 WFDD135 pKa = 3.49 RR136 pKa = 11.84 VGWPIVATLRR146 pKa = 11.84 NVTDD150 pKa = 4.35 PGTFMSPYY158 pKa = 8.77 LFAMASLEE166 pKa = 4.2 VEE168 pKa = 4.43 SS169 pKa = 4.53
Molecular weight: 18.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.745
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.757
Grimsley 3.656
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.05
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|Q854G1|Q854G1_BPMOM Uncharacterized protein OS=Mycobacterium phage Omega OX=205879 GN=106 PE=4 SV=1
MM1 pKa = 7.12 RR2 pKa = 11.84 TCTIPGCEE10 pKa = 4.32 KK11 pKa = 9.83 KK12 pKa = 9.56 HH13 pKa = 5.72 HH14 pKa = 6.39 ARR16 pKa = 11.84 GFCVVHH22 pKa = 5.64 YY23 pKa = 9.8 GRR25 pKa = 11.84 WTRR28 pKa = 11.84 SSDD31 pKa = 3.57 EE32 pKa = 3.44 PRR34 pKa = 11.84 GNYY37 pKa = 8.91 RR38 pKa = 11.84 RR39 pKa = 11.84 SHH41 pKa = 6.77 RR42 pKa = 11.84 SDD44 pKa = 5.46 CKK46 pKa = 11.15 LEE48 pKa = 3.74 WCNGRR53 pKa = 11.84 FYY55 pKa = 11.42 AKK57 pKa = 10.02 DD58 pKa = 3.06 LCRR61 pKa = 11.84 FHH63 pKa = 6.88 HH64 pKa = 6.03 QRR66 pKa = 11.84 LLRR69 pKa = 11.84 GKK71 pKa = 10.72 AMDD74 pKa = 4.31 DD75 pKa = 3.16 PRR77 pKa = 11.84 GNVGIFDD84 pKa = 4.07 WAACGTPAQYY94 pKa = 10.22 RR95 pKa = 11.84 KK96 pKa = 9.65 HH97 pKa = 5.68 FRR99 pKa = 11.84 HH100 pKa = 7.05 GIPLCDD106 pKa = 3.63 ACRR109 pKa = 11.84 KK110 pKa = 10.13 AEE112 pKa = 3.99 SRR114 pKa = 11.84 RR115 pKa = 11.84 QKK117 pKa = 10.83 DD118 pKa = 3.09 RR119 pKa = 11.84 RR120 pKa = 11.84 EE121 pKa = 3.73 KK122 pKa = 10.82 AKK124 pKa = 10.59 RR125 pKa = 3.35
Molecular weight: 14.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.37
IPC_protein 9.633
Toseland 10.321
ProMoST 10.072
Dawson 10.452
Bjellqvist 10.16
Wikipedia 10.613
Rodwell 10.76
Grimsley 10.496
Solomon 10.511
Lehninger 10.482
Nozaki 10.409
DTASelect 10.116
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.394
Patrickios 10.467
IPC_peptide 10.511
IPC2_peptide 9.472
IPC2.peptide.svr19 8.328
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
237
0
237
34708
22
1599
146.4
16.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.767 ± 0.236
1.513 ± 0.145
6.532 ± 0.161
7.033 ± 0.243
3.123 ± 0.117
8.903 ± 0.372
2.21 ± 0.1
4.832 ± 0.119
4.045 ± 0.157
8.099 ± 0.181
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.087
3.27 ± 0.104
5.474 ± 0.165
2.979 ± 0.177
6.811 ± 0.224
5.492 ± 0.167
5.382 ± 0.195
6.823 ± 0.177
2.233 ± 0.097
3.025 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here