Halobacteriovorax marinus (strain ATCC BAA-682 / DSM 15412 / SJ) (Bacteriovorax marinus)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3230 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1X637|E1X637_HALMS Putative integral membrane zinc-metalloprotease OS=Halobacteriovorax marinus (strain ATCC BAA-682 / DSM 15412 / SJ) OX=862908 GN=BMS_2595 PE=3 SV=1
MM1 pKa = 6.93 MVWNFRR7 pKa = 11.84 KK8 pKa = 10.14 ALTLMSLLTLFAACNSAKK26 pKa = 10.39 EE27 pKa = 4.25 SVEE30 pKa = 4.08 VVDD33 pKa = 3.34 QGRR36 pKa = 11.84 FNRR39 pKa = 11.84 PPVSTPDD46 pKa = 4.09 DD47 pKa = 3.83 PTNDD51 pKa = 4.61 DD52 pKa = 3.23 ISLVNSLVAITRR64 pKa = 11.84 EE65 pKa = 3.72 RR66 pKa = 11.84 LAPSDD71 pKa = 3.37 QAIITFTARR80 pKa = 11.84 DD81 pKa = 3.6 SDD83 pKa = 3.66 GVAINEE89 pKa = 4.36 GGLDD93 pKa = 3.31 VDD95 pKa = 5.19 FSLVGDD101 pKa = 4.2 GTSTGTFSATVDD113 pKa = 3.51 TGNGVYY119 pKa = 10.42 KK120 pKa = 10.25 ATLTGIDD127 pKa = 3.73 YY128 pKa = 10.8 GSKK131 pKa = 8.14 NTIRR135 pKa = 11.84 VEE137 pKa = 4.27 VEE139 pKa = 3.5 GSTLIFVQPLQLQVISGDD157 pKa = 3.66 YY158 pKa = 10.12 YY159 pKa = 11.32 RR160 pKa = 11.84 EE161 pKa = 3.72 IDD163 pKa = 4.32 LDD165 pKa = 3.68 NSTDD169 pKa = 3.27 QDD171 pKa = 3.5 EE172 pKa = 4.58 FQIEE176 pKa = 4.5 VNLTTSNFDD185 pKa = 3.31 YY186 pKa = 11.4 SNAEE190 pKa = 3.92 SAGEE194 pKa = 4.02 DD195 pKa = 2.92 IRR197 pKa = 11.84 FFDD200 pKa = 4.89 EE201 pKa = 5.81 DD202 pKa = 4.32 FNEE205 pKa = 3.72 QDD207 pKa = 3.06 FWIEE211 pKa = 3.71 NWDD214 pKa = 3.47 NTGDD218 pKa = 3.57 SKK220 pKa = 11.2 IWVKK224 pKa = 10.2 VQNSGTDD231 pKa = 3.17 KK232 pKa = 11.25 LLLVYY237 pKa = 10.48 GNNSLASASSRR248 pKa = 11.84 EE249 pKa = 4.14 GVFSYY254 pKa = 10.94 DD255 pKa = 3.44 VNKK258 pKa = 10.53 DD259 pKa = 2.72 IYY261 pKa = 11.19 YY262 pKa = 10.28 EE263 pKa = 4.26 LSQVAGPRR271 pKa = 11.84 NYY273 pKa = 10.62 GISSYY278 pKa = 10.81 ISNNNVDD285 pKa = 3.62 VLTNAGYY292 pKa = 10.54 SSQTISPVAATTYY305 pKa = 10.88 SNVISGLIGVDD316 pKa = 3.96 GPISGRR322 pKa = 11.84 FLTFNDD328 pKa = 4.17 GADD331 pKa = 3.48 TVAPLSFASTTLAYY345 pKa = 8.8 PKK347 pKa = 10.64 SRR349 pKa = 11.84 GTDD352 pKa = 2.72 DD353 pKa = 3.15 WDD355 pKa = 3.38 IYY357 pKa = 11.12 NPNSVTANFTLSNYY371 pKa = 9.98 DD372 pKa = 3.03 SSGNLVNSNSYY383 pKa = 10.88 SLAAGAEE390 pKa = 3.82 LHH392 pKa = 6.68 IDD394 pKa = 3.47 YY395 pKa = 10.56 DD396 pKa = 4.05 VSKK399 pKa = 10.07 MGLIEE404 pKa = 4.36 SDD406 pKa = 4.0 YY407 pKa = 10.84 PLLGLYY413 pKa = 8.46 YY414 pKa = 10.36 QSNSNDD420 pKa = 3.18 AVVMMKK426 pKa = 10.23 PSTDD430 pKa = 3.27 IIGPAATSGSIAIVEE445 pKa = 4.6 DD446 pKa = 3.81 GTNGTIYY453 pKa = 8.97 YY454 pKa = 10.11 TNGTTQAFSGDD465 pKa = 3.6 KK466 pKa = 10.85 GDD468 pKa = 4.63 VIDD471 pKa = 4.64 FSGGGSQNSATGLRR485 pKa = 11.84 VIATKK490 pKa = 10.5 GVSAVSQADD499 pKa = 3.42 SDD501 pKa = 4.18 GSEE504 pKa = 4.39 SASFWPIEE512 pKa = 4.2 EE513 pKa = 4.85 LNNDD517 pKa = 4.1 YY518 pKa = 10.56 IVPASSQYY526 pKa = 11.0 VVIICPEE533 pKa = 4.12 TVNITLTDD541 pKa = 3.57 PGGNDD546 pKa = 3.06 NSGVCVPAGGNNPGVISFGSATVVSFQDD574 pKa = 3.54 GTRR577 pKa = 11.84 VSGDD581 pKa = 3.17 ANFFMYY587 pKa = 10.61 YY588 pKa = 9.97 EE589 pKa = 4.4 YY590 pKa = 10.88 EE591 pKa = 5.4 DD592 pKa = 3.99 EE593 pKa = 5.85 DD594 pKa = 3.9 EE595 pKa = 4.6 TNVVSWKK602 pKa = 8.11 QARR605 pKa = 11.84 SYY607 pKa = 11.46 SPVPVSTSVGAEE619 pKa = 3.95 QSWDD623 pKa = 3.21
Molecular weight: 66.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.579
ProMoST 3.948
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.923
Patrickios 1.443
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|E1X1N0|E1X1N0_HALMS Putative integral membrane protein OS=Halobacteriovorax marinus (strain ATCC BAA-682 / DSM 15412 / SJ) OX=862908 GN=BMS_0007 PE=4 SV=1
MM1 pKa = 7.83 SKK3 pKa = 9.05 RR4 pKa = 11.84 TWQPKK9 pKa = 7.64 RR10 pKa = 11.84 KK11 pKa = 9.41 KK12 pKa = 9.57 RR13 pKa = 11.84 LRR15 pKa = 11.84 VHH17 pKa = 6.74 GFLKK21 pKa = 10.62 RR22 pKa = 11.84 MSTAGGKK29 pKa = 9.27 NVINARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.01 GRR40 pKa = 11.84 KK41 pKa = 8.14 QLTVSTGSKK50 pKa = 10.24
Molecular weight: 5.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3230
0
3230
1060232
33
3358
328.2
37.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.818 ± 0.043
1.105 ± 0.017
5.543 ± 0.031
7.371 ± 0.052
5.237 ± 0.039
6.11 ± 0.045
1.882 ± 0.022
7.527 ± 0.04
8.229 ± 0.052
9.931 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.238 ± 0.023
5.282 ± 0.036
3.161 ± 0.025
2.986 ± 0.024
4.103 ± 0.029
8.003 ± 0.049
4.983 ± 0.039
5.956 ± 0.039
0.919 ± 0.014
3.615 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here