Firmicutes bacterium CAG:24

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5H0B2|R5H0B2_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:24 OX=1263012 GN=BN555_00317 PE=4 SV=1
MM1 pKa = 7.5SMKK4 pKa = 10.55KK5 pKa = 10.16LGSILLSTAMVMSLAACGSQNTSAPAATTDD35 pKa = 3.54DD36 pKa = 3.86SATKK40 pKa = 10.27EE41 pKa = 4.07EE42 pKa = 4.23AVAPAEE48 pKa = 4.28SAVAADD54 pKa = 3.94TDD56 pKa = 4.11VSGSTLEE63 pKa = 4.09VAVTYY68 pKa = 10.21TGDD71 pKa = 3.31QATTFQGLVDD81 pKa = 4.02KK82 pKa = 11.21FEE84 pKa = 4.21EE85 pKa = 4.88EE86 pKa = 4.25YY87 pKa = 11.23GVTVNIAEE95 pKa = 4.29YY96 pKa = 11.04GDD98 pKa = 5.13DD99 pKa = 4.15YY100 pKa = 11.87EE101 pKa = 4.53NTLKK105 pKa = 9.92TRR107 pKa = 11.84MASNEE112 pKa = 4.09LPDD115 pKa = 3.65VFQTHH120 pKa = 5.39GWSLLRR126 pKa = 11.84YY127 pKa = 9.47KK128 pKa = 10.27EE129 pKa = 4.11YY130 pKa = 11.16LMNLQDD136 pKa = 4.08QPWVSDD142 pKa = 3.66YY143 pKa = 11.59DD144 pKa = 3.88EE145 pKa = 4.79SALGVIQDD153 pKa = 4.5DD154 pKa = 4.02DD155 pKa = 4.0GAIYY159 pKa = 11.11VLMISEE165 pKa = 5.79LINGTLVNLDD175 pKa = 3.44ACDD178 pKa = 3.42AAGVDD183 pKa = 4.65PYY185 pKa = 11.29AIHH188 pKa = 6.29TWDD191 pKa = 5.19DD192 pKa = 3.41FTEE195 pKa = 4.38ACAKK199 pKa = 10.17IKK201 pKa = 10.12TSGLTPLNVVSNPGLLANFAGTFVSYY227 pKa = 10.01EE228 pKa = 3.92GEE230 pKa = 4.11MFEE233 pKa = 6.1DD234 pKa = 3.67SAAMLDD240 pKa = 5.56GSFDD244 pKa = 3.31WQDD247 pKa = 3.44YY248 pKa = 10.38KK249 pKa = 11.61DD250 pKa = 3.91SLVKK254 pKa = 10.65YY255 pKa = 6.7MANWIDD261 pKa = 2.94SGYY264 pKa = 10.79YY265 pKa = 9.74YY266 pKa = 10.97DD267 pKa = 6.52DD268 pKa = 4.06ILTMADD274 pKa = 2.98TDD276 pKa = 3.75LTEE279 pKa = 4.43RR280 pKa = 11.84FAAGKK285 pKa = 10.18GAFSLGNDD293 pKa = 3.57PSVMLTCLSLNPDD306 pKa = 2.75ARR308 pKa = 11.84FAFMPSFASKK318 pKa = 10.57EE319 pKa = 4.1GGKK322 pKa = 8.72EE323 pKa = 3.55HH324 pKa = 7.13VGIGEE329 pKa = 4.51GDD331 pKa = 3.41TFGIWKK337 pKa = 8.62DD338 pKa = 3.41TGNADD343 pKa = 3.39AAKK346 pKa = 10.67LFLEE350 pKa = 4.48YY351 pKa = 9.53MARR354 pKa = 11.84PEE356 pKa = 4.13VAQEE360 pKa = 3.69MNAATGKK367 pKa = 10.06ISCLKK372 pKa = 9.54STMEE376 pKa = 4.23IDD378 pKa = 3.74DD379 pKa = 4.27SYY381 pKa = 11.6GLQVFQEE388 pKa = 4.34MKK390 pKa = 10.12EE391 pKa = 3.93KK392 pKa = 10.92CADD395 pKa = 3.41CDD397 pKa = 3.29IFYY400 pKa = 10.88EE401 pKa = 4.24NLWDD405 pKa = 3.86RR406 pKa = 11.84QYY408 pKa = 10.45MPSGMWSIFGNASDD422 pKa = 4.64MLFDD426 pKa = 5.85DD427 pKa = 5.48DD428 pKa = 5.84SEE430 pKa = 5.42AGQDD434 pKa = 3.57EE435 pKa = 4.87VIEE438 pKa = 4.12YY439 pKa = 10.85LLEE442 pKa = 4.39NYY444 pKa = 9.8QDD446 pKa = 4.74LYY448 pKa = 11.56DD449 pKa = 4.3AAQEE453 pKa = 4.29GG454 pKa = 3.97

Molecular weight: 49.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5H0Z3|R5H0Z3_9FIRM Aldose 1-epimerase OS=Firmicutes bacterium CAG:24 OX=1263012 GN=BN555_01445 PE=3 SV=1
MM1 pKa = 7.69TIQKK5 pKa = 9.78RR6 pKa = 11.84FFIIFFRR13 pKa = 11.84IFFYY17 pKa = 10.94VSPSSDD23 pKa = 2.61RR24 pKa = 11.84RR25 pKa = 11.84QRR27 pKa = 11.84VHH29 pKa = 6.09NCNIIYY35 pKa = 10.05RR36 pKa = 11.84LCFSKK41 pKa = 11.07NSFEE45 pKa = 4.14RR46 pKa = 11.84MSVLFRR52 pKa = 11.84QRR54 pKa = 11.84IKK56 pKa = 10.81RR57 pKa = 11.84IDD59 pKa = 3.34FEE61 pKa = 4.45YY62 pKa = 9.91FIRR65 pKa = 11.84MLQKK69 pKa = 10.12FQTQIQCQIAFSASSRR85 pKa = 11.84RR86 pKa = 11.84LYY88 pKa = 10.58KK89 pKa = 10.11INTVFFCSLQIFFCFQKK106 pKa = 10.72EE107 pKa = 4.29LISEE111 pKa = 4.85DD112 pKa = 3.21IDD114 pKa = 4.06LLFTIPFLSRR124 pKa = 11.84ITFFSILPNRR134 pKa = 11.84FHH136 pKa = 7.12QRR138 pKa = 11.84RR139 pKa = 11.84TLINLPEE146 pKa = 4.6FGSVSLSKK154 pKa = 9.74TIHH157 pKa = 6.24LKK159 pKa = 9.15STVKK163 pKa = 8.85FCRR166 pKa = 11.84RR167 pKa = 11.84IVVTGYY173 pKa = 8.51QNRR176 pKa = 11.84IFQTDD181 pKa = 2.89KK182 pKa = 10.82KK183 pKa = 9.89IVYY186 pKa = 9.98NIVFCSPKK194 pKa = 9.92RR195 pKa = 11.84FSAHH199 pKa = 5.97KK200 pKa = 9.63VLIYY204 pKa = 8.08TFRR207 pKa = 11.84YY208 pKa = 8.9RR209 pKa = 11.84DD210 pKa = 3.74LVKK213 pKa = 10.39KK214 pKa = 10.36AQLGKK219 pKa = 9.95SRR221 pKa = 11.84NRR223 pKa = 11.84FFFPLSQQFLCFQLKK238 pKa = 9.13VFQICFHH245 pKa = 6.49RR246 pKa = 11.84FEE248 pKa = 4.45IAVMFCDD255 pKa = 5.05YY256 pKa = 10.65IRR258 pKa = 11.84QFPSKK263 pKa = 10.17CNQRR267 pKa = 11.84FLLLFFHH274 pKa = 7.55FIEE277 pKa = 5.06TIFNFII283 pKa = 4.16

Molecular weight: 34.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2518

0

2518

809563

29

1630

321.5

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.225 ± 0.053

1.629 ± 0.018

5.485 ± 0.04

7.59 ± 0.055

4.18 ± 0.032

7.282 ± 0.039

1.788 ± 0.021

6.565 ± 0.042

6.235 ± 0.031

9.463 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.985 ± 0.022

3.832 ± 0.03

3.451 ± 0.026

3.65 ± 0.028

4.785 ± 0.041

5.628 ± 0.036

5.266 ± 0.028

6.904 ± 0.041

1.088 ± 0.02

3.967 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski