Amino acid dipepetide frequency for Firmicutes bacterium CAG:24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.436AlaAla: 9.436 ± 0.183	1.334AlaCys: 1.334 ± 0.04	5.194AlaAsp: 5.194 ± 0.091	6.053AlaGlu: 6.053 ± 0.105	3.377AlaPhe: 3.377 ± 0.063	6.974AlaGly: 6.974 ± 0.106	1.253AlaHis: 1.253 ± 0.041	4.757AlaIle: 4.757 ± 0.083	4.74AlaLys: 4.74 ± 0.096	7.73AlaLeu: 7.73 ± 0.111	2.438AlaMet: 2.438 ± 0.059	2.417AlaAsn: 2.417 ± 0.065	2.34AlaPro: 2.34 ± 0.061	3.196AlaGln: 3.196 ± 0.069	3.266AlaArg: 3.266 ± 0.064	4.033AlaSer: 4.033 ± 0.076	3.072AlaThr: 3.072 ± 0.076	6.953AlaVal: 6.953 ± 0.121	0.865AlaTrp: 0.865 ± 0.038	2.818AlaTyr: 2.818 ± 0.071	0.001AlaXaa: 0.001 ± 0.001
Cys	1.219CysAla: 1.219 ± 0.039	0.356CysCys: 0.356 ± 0.021	0.829CysAsp: 0.829 ± 0.034	0.952CysGlu: 0.952 ± 0.035	0.715CysPhe: 0.715 ± 0.031	1.586CysGly: 1.586 ± 0.044	0.33CysHis: 0.33 ± 0.02	1.043CysIle: 1.043 ± 0.039	0.742CysLys: 0.742 ± 0.032	1.484CysLeu: 1.484 ± 0.045	0.458CysMet: 0.458 ± 0.022	0.542CysAsn: 0.542 ± 0.028	0.687CysPro: 0.687 ± 0.038	0.521CysGln: 0.521 ± 0.028	0.993CysArg: 0.993 ± 0.039	1.041CysSer: 1.041 ± 0.034	0.73CysThr: 0.73 ± 0.034	1.206CysVal: 1.206 ± 0.046	0.175CysTrp: 0.175 ± 0.016	0.674CysTyr: 0.674 ± 0.029	0.001CysXaa: 0.001 ± 0.001
Asp	4.614AspAla: 4.614 ± 0.093	0.855AspCys: 0.855 ± 0.036	2.574AspAsp: 2.574 ± 0.075	4.096AspGlu: 4.096 ± 0.076	2.515AspPhe: 2.515 ± 0.057	4.829AspGly: 4.829 ± 0.083	0.936AspHis: 0.936 ± 0.031	3.602AspIle: 3.602 ± 0.064	2.856AspLys: 2.856 ± 0.071	4.659AspLeu: 4.659 ± 0.087	1.648AspMet: 1.648 ± 0.049	1.92AspAsn: 1.92 ± 0.056	2.049AspPro: 2.049 ± 0.047	1.649AspGln: 1.649 ± 0.047	2.762AspArg: 2.762 ± 0.062	3.079AspSer: 3.079 ± 0.062	3.056AspThr: 3.056 ± 0.066	3.858AspVal: 3.858 ± 0.071	0.742AspTrp: 0.742 ± 0.033	2.547AspTyr: 2.547 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	6.448GluAla: 6.448 ± 0.099	0.823GluCys: 0.823 ± 0.029	4.12GluAsp: 4.12 ± 0.091	7.242GluGlu: 7.242 ± 0.132	2.446GluPhe: 2.446 ± 0.053	4.342GluGly: 4.342 ± 0.064	1.416GluHis: 1.416 ± 0.047	5.16GluIle: 5.16 ± 0.088	6.909GluLys: 6.909 ± 0.11	6.82GluLeu: 6.82 ± 0.117	2.28GluMet: 2.28 ± 0.056	3.876GluAsn: 3.876 ± 0.072	2.041GluPro: 2.041 ± 0.05	3.202GluGln: 3.202 ± 0.067	3.616GluArg: 3.616 ± 0.071	3.308GluSer: 3.308 ± 0.08	3.998GluThr: 3.998 ± 0.083	4.243GluVal: 4.243 ± 0.087	0.751GluTrp: 0.751 ± 0.031	2.857GluTyr: 2.857 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	3.343PheAla: 3.343 ± 0.069	0.904PheCys: 0.904 ± 0.035	2.461PheAsp: 2.461 ± 0.055	2.69PheGlu: 2.69 ± 0.053	2.107PhePhe: 2.107 ± 0.061	3.257PheGly: 3.257 ± 0.074	0.919PheHis: 0.919 ± 0.035	2.293PheIle: 2.293 ± 0.061	1.543PheLys: 1.543 ± 0.053	4.565PheLeu: 4.565 ± 0.077	1.083PheMet: 1.083 ± 0.038	1.313PheAsn: 1.313 ± 0.038	1.529PhePro: 1.529 ± 0.044	1.388PheGln: 1.388 ± 0.042	1.812PheArg: 1.812 ± 0.046	3.0PheSer: 3.0 ± 0.069	2.233PheThr: 2.233 ± 0.051	2.881PheVal: 2.881 ± 0.066	0.588PheTrp: 0.588 ± 0.033	1.89PheTyr: 1.89 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	5.356GlyAla: 5.356 ± 0.098	1.47GlyCys: 1.47 ± 0.046	3.391GlyAsp: 3.391 ± 0.069	4.716GlyGlu: 4.716 ± 0.074	3.228GlyPhe: 3.228 ± 0.066	4.809GlyGly: 4.809 ± 0.093	1.324GlyHis: 1.324 ± 0.042	6.05GlyIle: 6.05 ± 0.098	5.484GlyLys: 5.484 ± 0.089	6.244GlyLeu: 6.244 ± 0.091	2.745GlyMet: 2.745 ± 0.056	2.841GlyAsn: 2.841 ± 0.063	1.353GlyPro: 1.353 ± 0.045	2.643GlyGln: 2.643 ± 0.058	3.559GlyArg: 3.559 ± 0.068	4.217GlySer: 4.217 ± 0.078	4.374GlyThr: 4.374 ± 0.067	4.942GlyVal: 4.942 ± 0.092	0.828GlyTrp: 0.828 ± 0.032	3.247GlyTyr: 3.247 ± 0.069	0.0GlyXaa: 0.0 ± 0.0
His	1.286HisAla: 1.286 ± 0.046	0.348HisCys: 0.348 ± 0.021	0.945HisAsp: 0.945 ± 0.038	1.024HisGlu: 1.024 ± 0.035	0.954HisPhe: 0.954 ± 0.032	1.355HisGly: 1.355 ± 0.041	0.42HisHis: 0.42 ± 0.044	1.175HisIle: 1.175 ± 0.033	0.837HisLys: 0.837 ± 0.033	1.648HisLeu: 1.648 ± 0.049	0.555HisMet: 0.555 ± 0.024	0.705HisAsn: 0.705 ± 0.032	0.97HisPro: 0.97 ± 0.038	0.598HisGln: 0.598 ± 0.023	0.934HisArg: 0.934 ± 0.032	0.952HisSer: 0.952 ± 0.032	0.946HisThr: 0.946 ± 0.037	1.228HisVal: 1.228 ± 0.039	0.195HisTrp: 0.195 ± 0.015	0.808HisTyr: 0.808 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.651IleAla: 5.651 ± 0.095	1.345IleCys: 1.345 ± 0.041	3.501IleAsp: 3.501 ± 0.072	4.163IleGlu: 4.163 ± 0.068	2.83IlePhe: 2.83 ± 0.07	5.105IleGly: 5.105 ± 0.095	1.281IleHis: 1.281 ± 0.038	3.703IleIle: 3.703 ± 0.088	2.884IleLys: 2.884 ± 0.066	6.958IleLeu: 6.958 ± 0.111	1.529IleMet: 1.529 ± 0.049	2.215IleAsn: 2.215 ± 0.048	2.963IlePro: 2.963 ± 0.057	1.966IleGln: 1.966 ± 0.043	3.876IleArg: 3.876 ± 0.078	4.404IleSer: 4.404 ± 0.068	3.473IleThr: 3.473 ± 0.067	4.652IleVal: 4.652 ± 0.077	0.714IleTrp: 0.714 ± 0.031	2.436IleTyr: 2.436 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	4.764LysAla: 4.764 ± 0.094	0.655LysCys: 0.655 ± 0.033	3.557LysAsp: 3.557 ± 0.065	6.118LysGlu: 6.118 ± 0.1	1.553LysPhe: 1.553 ± 0.045	3.784LysGly: 3.784 ± 0.073	0.945LysHis: 0.945 ± 0.033	4.263LysIle: 4.263 ± 0.08	5.807LysLys: 5.807 ± 0.094	5.289LysLeu: 5.289 ± 0.084	1.865LysMet: 1.865 ± 0.047	3.238LysAsn: 3.238 ± 0.07	1.881LysPro: 1.881 ± 0.053	2.524LysGln: 2.524 ± 0.061	3.078LysArg: 3.078 ± 0.062	2.934LysSer: 2.934 ± 0.061	3.536LysThr: 3.536 ± 0.065	3.618LysVal: 3.618 ± 0.073	0.6LysTrp: 0.6 ± 0.027	2.344LysTyr: 2.344 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	7.498LeuAla: 7.498 ± 0.114	1.829LeuCys: 1.829 ± 0.049	5.079LeuAsp: 5.079 ± 0.072	6.736LeuGlu: 6.736 ± 0.112	4.325LeuPhe: 4.325 ± 0.089	5.997LeuGly: 5.997 ± 0.092	1.687LeuHis: 1.687 ± 0.046	5.87LeuIle: 5.87 ± 0.1	5.754LeuLys: 5.754 ± 0.09	9.674LeuLeu: 9.674 ± 0.149	2.781LeuMet: 2.781 ± 0.056	3.529LeuAsn: 3.529 ± 0.064	3.901LeuPro: 3.901 ± 0.077	3.598LeuGln: 3.598 ± 0.062	4.038LeuArg: 4.038 ± 0.081	6.392LeuSer: 6.392 ± 0.103	5.47LeuThr: 5.47 ± 0.081	5.775LeuVal: 5.775 ± 0.08	0.959LeuTrp: 0.959 ± 0.034	3.734LeuTyr: 3.734 ± 0.073	0.0LeuXaa: 0.0 ± 0.0
Met	2.379MetAla: 2.379 ± 0.058	0.327MetCys: 0.327 ± 0.018	1.734MetAsp: 1.734 ± 0.044	2.767MetGlu: 2.767 ± 0.062	0.986MetPhe: 0.986 ± 0.036	2.063MetGly: 2.063 ± 0.053	0.463MetHis: 0.463 ± 0.022	1.923MetIle: 1.923 ± 0.053	2.251MetLys: 2.251 ± 0.054	2.755MetLeu: 2.755 ± 0.059	0.899MetMet: 0.899 ± 0.039	1.323MetAsn: 1.323 ± 0.044	1.139MetPro: 1.139 ± 0.036	1.29MetGln: 1.29 ± 0.035	1.283MetArg: 1.283 ± 0.036	1.638MetSer: 1.638 ± 0.047	1.733MetThr: 1.733 ± 0.047	1.854MetVal: 1.854 ± 0.046	0.229MetTrp: 0.229 ± 0.017	0.815MetTyr: 0.815 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.165AsnAla: 3.165 ± 0.065	0.65AsnCys: 0.65 ± 0.031	1.833AsnAsp: 1.833 ± 0.048	2.311AsnGlu: 2.311 ± 0.052	1.476AsnPhe: 1.476 ± 0.044	3.559AsnGly: 3.559 ± 0.072	0.719AsnHis: 0.719 ± 0.028	2.556AsnIle: 2.556 ± 0.062	1.782AsnLys: 1.782 ± 0.045	3.496AsnLeu: 3.496 ± 0.064	1.098AsnMet: 1.098 ± 0.034	1.349AsnAsn: 1.349 ± 0.05	1.947AsnPro: 1.947 ± 0.053	1.412AsnGln: 1.412 ± 0.046	2.154AsnArg: 2.154 ± 0.062	2.063AsnSer: 2.063 ± 0.051	2.094AsnThr: 2.094 ± 0.055	2.663AsnVal: 2.663 ± 0.064	0.443AsnTrp: 0.443 ± 0.025	1.553AsnTyr: 1.553 ± 0.048	0.001AsnXaa: 0.001 ± 0.001
Pro	2.9ProAla: 2.9 ± 0.067	0.483ProCys: 0.483 ± 0.027	2.5ProAsp: 2.5 ± 0.059	3.576ProGlu: 3.576 ± 0.076	1.586ProPhe: 1.586 ± 0.047	2.677ProGly: 2.677 ± 0.062	0.614ProHis: 0.614 ± 0.027	1.953ProIle: 1.953 ± 0.057	1.821ProLys: 1.821 ± 0.052	2.869ProLeu: 2.869 ± 0.058	0.956ProMet: 0.956 ± 0.034	1.166ProAsn: 1.166 ± 0.032	0.697ProPro: 0.697 ± 0.03	1.169ProGln: 1.169 ± 0.038	1.017ProArg: 1.017 ± 0.036	1.768ProSer: 1.768 ± 0.045	1.582ProThr: 1.582 ± 0.047	3.202ProVal: 3.202 ± 0.055	0.394ProTrp: 0.394 ± 0.023	1.581ProTyr: 1.581 ± 0.05	0.001ProXaa: 0.001 ± 0.001
Gln	2.867GlnAla: 2.867 ± 0.059	0.371GlnCys: 0.371 ± 0.023	1.844GlnAsp: 1.844 ± 0.044	3.383GlnGlu: 3.383 ± 0.074	1.281GlnPhe: 1.281 ± 0.035	2.022GlnGly: 2.022 ± 0.049	0.547GlnHis: 0.547 ± 0.025	2.803GlnIle: 2.803 ± 0.063	3.176GlnLys: 3.176 ± 0.068	3.375GlnLeu: 3.375 ± 0.06	1.269GlnMet: 1.269 ± 0.041	1.737GlnAsn: 1.737 ± 0.046	1.07GlnPro: 1.07 ± 0.043	1.517GlnGln: 1.517 ± 0.056	1.584GlnArg: 1.584 ± 0.048	1.703GlnSer: 1.703 ± 0.048	2.065GlnThr: 2.065 ± 0.056	2.227GlnVal: 2.227 ± 0.05	0.343GlnTrp: 0.343 ± 0.025	1.319GlnTyr: 1.319 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	3.155ArgAla: 3.155 ± 0.063	0.704ArgCys: 0.704 ± 0.033	2.279ArgAsp: 2.279 ± 0.059	4.106ArgGlu: 4.106 ± 0.083	2.141ArgPhe: 2.141 ± 0.054	2.527ArgGly: 2.527 ± 0.061	0.863ArgHis: 0.863 ± 0.029	3.713ArgIle: 3.713 ± 0.072	3.504ArgLys: 3.504 ± 0.073	4.507ArgLeu: 4.507 ± 0.079	1.808ArgMet: 1.808 ± 0.048	1.849ArgAsn: 1.849 ± 0.049	1.508ArgPro: 1.508 ± 0.05	2.101ArgGln: 2.101 ± 0.055	2.747ArgArg: 2.747 ± 0.07	2.485ArgSer: 2.485 ± 0.057	2.412ArgThr: 2.412 ± 0.06	3.034ArgVal: 3.034 ± 0.062	0.413ArgTrp: 0.413 ± 0.023	1.995ArgTyr: 1.995 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	4.837SerAla: 4.837 ± 0.089	0.991SerCys: 0.991 ± 0.038	3.184SerAsp: 3.184 ± 0.064	3.671SerGlu: 3.671 ± 0.069	2.732SerPhe: 2.732 ± 0.064	4.976SerGly: 4.976 ± 0.084	0.957SerHis: 0.957 ± 0.034	3.713SerIle: 3.713 ± 0.075	2.768SerLys: 2.768 ± 0.06	5.073SerLeu: 5.073 ± 0.093	1.707SerMet: 1.707 ± 0.051	1.862SerAsn: 1.862 ± 0.051	1.75SerPro: 1.75 ± 0.046	1.734SerGln: 1.734 ± 0.045	2.956SerArg: 2.956 ± 0.063	3.26SerSer: 3.26 ± 0.082	2.694SerThr: 2.694 ± 0.065	4.407SerVal: 4.407 ± 0.067	0.668SerTrp: 0.668 ± 0.031	2.337SerTyr: 2.337 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	5.046ThrAla: 5.046 ± 0.08	0.669ThrCys: 0.669 ± 0.024	3.235ThrAsp: 3.235 ± 0.071	3.933ThrGlu: 3.933 ± 0.078	2.102ThrPhe: 2.102 ± 0.048	4.707ThrGly: 4.707 ± 0.08	0.877ThrHis: 0.877 ± 0.029	3.401ThrIle: 3.401 ± 0.078	2.868ThrLys: 2.868 ± 0.066	4.794ThrLeu: 4.794 ± 0.08	1.345ThrMet: 1.345 ± 0.041	1.768ThrAsn: 1.768 ± 0.051	2.092ThrPro: 2.092 ± 0.054	1.68ThrGln: 1.68 ± 0.047	1.999ThrArg: 1.999 ± 0.048	2.674ThrSer: 2.674 ± 0.063	2.647ThrThr: 2.647 ± 0.067	4.368ThrVal: 4.368 ± 0.074	0.587ThrTrp: 0.587 ± 0.029	1.864ThrTyr: 1.864 ± 0.053	0.001ThrXaa: 0.001 ± 0.001
Val	4.716ValAla: 4.716 ± 0.088	1.351ValCys: 1.351 ± 0.041	3.544ValAsp: 3.544 ± 0.065	4.554ValGlu: 4.554 ± 0.088	3.171ValPhe: 3.171 ± 0.067	4.484ValGly: 4.484 ± 0.084	1.223ValHis: 1.223 ± 0.042	4.701ValIle: 4.701 ± 0.086	4.007ValLys: 4.007 ± 0.076	7.599ValLeu: 7.599 ± 0.11	1.965ValMet: 1.965 ± 0.054	2.566ValAsn: 2.566 ± 0.052	2.736ValPro: 2.736 ± 0.06	2.118ValGln: 2.118 ± 0.04	3.417ValArg: 3.417 ± 0.055	4.619ValSer: 4.619 ± 0.069	4.015ValThr: 4.015 ± 0.074	4.775ValVal: 4.775 ± 0.09	0.815ValTrp: 0.815 ± 0.032	2.663ValTyr: 2.663 ± 0.053	0.001ValXaa: 0.001 ± 0.001
Trp	0.644TrpAla: 0.644 ± 0.03	0.193TrpCys: 0.193 ± 0.014	0.588TrpAsp: 0.588 ± 0.03	0.794TrpGlu: 0.794 ± 0.036	0.478TrpPhe: 0.478 ± 0.023	0.739TrpGly: 0.739 ± 0.037	0.225TrpHis: 0.225 ± 0.017	0.777TrpIle: 0.777 ± 0.033	0.898TrpLys: 0.898 ± 0.035	1.11TrpLeu: 1.11 ± 0.045	0.429TrpMet: 0.429 ± 0.022	0.615TrpAsn: 0.615 ± 0.029	0.277TrpPro: 0.277 ± 0.018	0.504TrpGln: 0.504 ± 0.025	0.435TrpArg: 0.435 ± 0.023	0.514TrpSer: 0.514 ± 0.027	0.493TrpThr: 0.493 ± 0.025	0.568TrpVal: 0.568 ± 0.028	0.132TrpTrp: 0.132 ± 0.013	0.473TrpTyr: 0.473 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.921TyrAla: 2.921 ± 0.061	0.627TyrCys: 0.627 ± 0.034	2.453TyrAsp: 2.453 ± 0.063	3.008TyrGlu: 3.008 ± 0.058	1.799TyrPhe: 1.799 ± 0.047	3.028TyrGly: 3.028 ± 0.061	0.882TyrHis: 0.882 ± 0.038	2.196TyrIle: 2.196 ± 0.058	1.722TyrLys: 1.722 ± 0.047	3.975TyrLeu: 3.975 ± 0.077	0.991TyrMet: 0.991 ± 0.035	1.493TyrAsn: 1.493 ± 0.046	1.582TyrPro: 1.582 ± 0.042	1.694TyrGln: 1.694 ± 0.049	2.328TyrArg: 2.328 ± 0.056	2.193TyrSer: 2.193 ± 0.064	2.033TyrThr: 2.033 ± 0.059	2.588TyrVal: 2.588 ± 0.056	0.443TyrTrp: 0.443 ± 0.026	1.716TyrTyr: 1.716 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.012XaaXaa: 0.012 ± 0.006

Statistics based on 2518 proteins (809563 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---