Bos taurus papillomavirus 11
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1CGB9|E1CGB9_BPV3 Replication protein E1 OS=Bos taurus papillomavirus 11 OX=714200 GN=E1 PE=3 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.31 GPNVTLEE9 pKa = 4.6 GIAADD14 pKa = 4.43 LEE16 pKa = 4.64 NAVSPINLNCEE27 pKa = 4.03 EE28 pKa = 4.78 EE29 pKa = 4.11 IDD31 pKa = 3.97 TEE33 pKa = 4.47 EE34 pKa = 4.48 VEE36 pKa = 4.49 LPNPFEE42 pKa = 4.17 ITATCYY48 pKa = 10.01 VCEE51 pKa = 4.78 KK52 pKa = 9.96 ILRR55 pKa = 11.84 LAVVTSNDD63 pKa = 4.67 GIHH66 pKa = 6.35 QLQQLLFEE74 pKa = 4.88 NLSLLCSACSRR85 pKa = 11.84 DD86 pKa = 3.58 VFCNRR91 pKa = 11.84 RR92 pKa = 11.84 PVKK95 pKa = 10.6 NGPP98 pKa = 3.32
Molecular weight: 10.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.313
IPC2_protein 4.495
IPC_protein 4.329
Toseland 4.19
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.113
Rodwell 4.177
Grimsley 4.101
Solomon 4.253
Lehninger 4.202
Nozaki 4.38
DTASelect 4.457
Thurlkill 4.19
EMBOSS 4.126
Sillero 4.444
Patrickios 3.032
IPC_peptide 4.266
IPC2_peptide 4.431
IPC2.peptide.svr19 4.377
Protein with the highest isoelectric point:
>tr|E1CGC1|E1CGC1_BPV3 E4 protein OS=Bos taurus papillomavirus 11 OX=714200 GN=E4 PE=4 SV=1
MM1 pKa = 7.71 ASLEE5 pKa = 4.03 ARR7 pKa = 11.84 FDD9 pKa = 3.74 AVQEE13 pKa = 4.17 SLLQIYY19 pKa = 9.7 EE20 pKa = 4.37 SEE22 pKa = 4.3 SSSLEE27 pKa = 3.43 LCYY30 pKa = 10.35 QYY32 pKa = 10.46 WQLIRR37 pKa = 11.84 KK38 pKa = 8.28 EE39 pKa = 3.74 NALYY43 pKa = 8.26 FTARR47 pKa = 11.84 QQGKK51 pKa = 7.31 TRR53 pKa = 11.84 LGLYY57 pKa = 9.26 AVPATRR63 pKa = 11.84 VSEE66 pKa = 4.33 QKK68 pKa = 11.0 AKK70 pKa = 10.84 DD71 pKa = 3.84 AIKK74 pKa = 8.27 MTLYY78 pKa = 10.92 LEE80 pKa = 4.44 SLKK83 pKa = 9.1 QTQFATEE90 pKa = 3.87 RR91 pKa = 11.84 WTLMDD96 pKa = 3.68 TSSEE100 pKa = 4.15 TFMAPPEE107 pKa = 4.08 NTFKK111 pKa = 10.99 KK112 pKa = 9.89 RR113 pKa = 11.84 GQHH116 pKa = 3.64 VTVVYY121 pKa = 10.8 DD122 pKa = 3.62 HH123 pKa = 7.53 DD124 pKa = 5.12 ANNSMLYY131 pKa = 8.97 TLWTEE136 pKa = 4.46 LYY138 pKa = 10.78 YY139 pKa = 10.93 LDD141 pKa = 5.72 TSDD144 pKa = 3.58 TWHH147 pKa = 6.62 KK148 pKa = 6.72 TTSSVDD154 pKa = 3.27 YY155 pKa = 11.11 DD156 pKa = 4.04 GIFYY160 pKa = 10.23 IDD162 pKa = 3.21 IYY164 pKa = 8.95 GTKK167 pKa = 9.03 IYY169 pKa = 10.3 YY170 pKa = 9.94 INFQDD175 pKa = 5.02 DD176 pKa = 3.2 ASLYY180 pKa = 9.77 SNSGQWEE187 pKa = 4.07 VHH189 pKa = 5.73 FEE191 pKa = 4.2 NKK193 pKa = 9.46 VLSPPVTSSLPVGTSGRR210 pKa = 11.84 RR211 pKa = 11.84 RR212 pKa = 11.84 AQTGDD217 pKa = 2.87 HH218 pKa = 6.23 AQPGGRR224 pKa = 11.84 KK225 pKa = 8.78 PSQLAGDD232 pKa = 3.78 SRR234 pKa = 11.84 RR235 pKa = 11.84 RR236 pKa = 11.84 SQSQSSSRR244 pKa = 11.84 SRR246 pKa = 11.84 SRR248 pKa = 11.84 SRR250 pKa = 11.84 SPTKK254 pKa = 9.9 EE255 pKa = 3.55 PHH257 pKa = 5.21 SGRR260 pKa = 11.84 RR261 pKa = 11.84 EE262 pKa = 3.79 AGIPGQQQGRR272 pKa = 11.84 PPGRR276 pKa = 11.84 RR277 pKa = 11.84 RR278 pKa = 11.84 GEE280 pKa = 4.05 SPEE283 pKa = 3.53 QRR285 pKa = 11.84 IFGGPSPPTPGQVGGRR301 pKa = 11.84 HH302 pKa = 5.92 RR303 pKa = 11.84 SPSSKK308 pKa = 8.71 PTSRR312 pKa = 11.84 LARR315 pKa = 11.84 LINEE319 pKa = 4.63 AYY321 pKa = 10.02 DD322 pKa = 3.8 PPVLLLQGPANILKK336 pKa = 9.87 CFRR339 pKa = 11.84 RR340 pKa = 11.84 RR341 pKa = 11.84 ATQTHH346 pKa = 5.11 GHH348 pKa = 6.74 RR349 pKa = 11.84 FLCMSTSWTWVCKK362 pKa = 9.63 TSPLKK367 pKa = 10.61 SGHH370 pKa = 6.3 RR371 pKa = 11.84 MLIAFTNCDD380 pKa = 3.01 QRR382 pKa = 11.84 KK383 pKa = 8.76 GFLSSVRR390 pKa = 11.84 LPKK393 pKa = 10.63 GVTFVKK399 pKa = 10.8 GSLTAYY405 pKa = 9.19 SICYY409 pKa = 9.24
Molecular weight: 46.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.88
IPC2_protein 9.282
IPC_protein 9.385
Toseland 9.721
ProMoST 9.589
Dawson 10.028
Bjellqvist 9.78
Wikipedia 10.248
Rodwell 10.233
Grimsley 10.131
Solomon 10.058
Lehninger 9.999
Nozaki 9.809
DTASelect 9.75
Thurlkill 9.853
EMBOSS 10.16
Sillero 9.955
Patrickios 5.105
IPC_peptide 10.043
IPC2_peptide 8.639
IPC2.peptide.svr19 8.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2424
98
606
346.3
38.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.817 ± 0.529
2.186 ± 0.531
5.899 ± 0.506
5.941 ± 0.622
4.703 ± 0.5
6.436 ± 0.715
2.269 ± 0.139
5.074 ± 0.585
4.827 ± 0.687
8.787 ± 0.974
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.939 ± 0.356
4.95 ± 0.755
6.477 ± 0.886
4.167 ± 0.458
6.559 ± 0.816
8.045 ± 0.789
5.899 ± 0.632
5.652 ± 0.636
1.526 ± 0.261
2.847 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here