Pseudooceanicola marinus
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4205 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X6Z365|A0A1X6Z365_9RHOB Uncharacterized protein OS=Pseudooceanicola marinus OX=396013 GN=PSM7751_01770 PE=4 SV=1
MM1 pKa = 7.14 ARR3 pKa = 11.84 MWILLLLAVATVSGCGGSSNPFDD26 pKa = 4.73 DD27 pKa = 5.32 DD28 pKa = 3.93 EE29 pKa = 5.12 VVGEE33 pKa = 4.56 PPDD36 pKa = 4.72 DD37 pKa = 4.49 SGDD40 pKa = 3.8 DD41 pKa = 3.85 GSPISSDD48 pKa = 3.34 RR49 pKa = 11.84 EE50 pKa = 4.14 LPPGTGTPEE59 pKa = 3.95 PDD61 pKa = 2.76 ASIFRR66 pKa = 11.84 RR67 pKa = 11.84 EE68 pKa = 4.04 GQTEE72 pKa = 4.02 DD73 pKa = 2.95 GGGYY77 pKa = 7.7 VTAVSYY83 pKa = 11.44 NSGDD87 pKa = 3.26 DD88 pKa = 3.52 TFIVDD93 pKa = 4.45 GLAFDD98 pKa = 5.49 GDD100 pKa = 3.49 NTYY103 pKa = 10.66 QRR105 pKa = 11.84 SQGATSTFGGYY116 pKa = 10.36 AIYY119 pKa = 10.31 EE120 pKa = 4.37 GAPSYY125 pKa = 10.84 QDD127 pKa = 3.57 SEE129 pKa = 4.38 TGAPIEE135 pKa = 4.24 QFLHH139 pKa = 5.71 RR140 pKa = 11.84 AIYY143 pKa = 9.71 GVSDD147 pKa = 3.44 SGQTEE152 pKa = 3.91 FAIVRR157 pKa = 11.84 SGNYY161 pKa = 9.45 AGYY164 pKa = 10.18 GFGGFVYY171 pKa = 10.22 QRR173 pKa = 11.84 NGSVTLPAPSTGQATYY189 pKa = 9.88 SGGYY193 pKa = 9.51 AGVRR197 pKa = 11.84 VFDD200 pKa = 3.68 GRR202 pKa = 11.84 SGIEE206 pKa = 3.84 YY207 pKa = 8.73 TQGDD211 pKa = 4.42 MTTSIDD217 pKa = 4.12 FEE219 pKa = 4.98 DD220 pKa = 4.76 FDD222 pKa = 5.16 GSGAVTGKK230 pKa = 7.82 ITNRR234 pKa = 11.84 RR235 pKa = 11.84 VFDD238 pKa = 3.74 EE239 pKa = 4.43 SGNDD243 pKa = 3.02 ITNDD247 pKa = 2.26 ITAAMDD253 pKa = 3.54 GTVTSLPVLDD263 pKa = 5.36 LVVAPNALDD272 pKa = 3.86 ANGEE276 pKa = 4.08 ATGGVVSRR284 pKa = 11.84 DD285 pKa = 3.58 PNDD288 pKa = 3.54 GDD290 pKa = 4.4 DD291 pKa = 4.32 YY292 pKa = 11.53 EE293 pKa = 4.58 TGTYY297 pKa = 10.04 YY298 pKa = 11.13 AVLSDD303 pKa = 4.73 GADD306 pKa = 3.27 NDD308 pKa = 3.69 LDD310 pKa = 3.97 AEE312 pKa = 4.29 EE313 pKa = 4.2 MVGIVVVTSTDD324 pKa = 3.24 PRR326 pKa = 11.84 DD327 pKa = 3.43 TDD329 pKa = 3.83 VEE331 pKa = 4.3 VQEE334 pKa = 4.27 TGGFILYY341 pKa = 10.0 RR342 pKa = 4.25
Molecular weight: 35.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.401
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.592
Rodwell 3.465
Grimsley 3.312
Solomon 3.63
Lehninger 3.592
Nozaki 3.745
DTASelect 4.024
Thurlkill 3.465
EMBOSS 3.592
Sillero 3.757
Patrickios 1.227
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A1X7ABF1|A0A1X7ABF1_9RHOB ATP-dependent DNA helicase RecG OS=Pseudooceanicola marinus OX=396013 GN=recG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4205
0
4205
1317918
29
4261
313.4
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.797 ± 0.056
0.863 ± 0.011
5.967 ± 0.043
6.27 ± 0.033
3.535 ± 0.024
8.928 ± 0.048
1.987 ± 0.019
4.754 ± 0.03
2.727 ± 0.035
10.494 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.022
2.273 ± 0.022
5.38 ± 0.035
3.137 ± 0.023
6.996 ± 0.05
5.049 ± 0.027
5.417 ± 0.028
7.106 ± 0.035
1.37 ± 0.015
2.163 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here