Clostridium phage susfortuna
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385IRI6|A0A385IRI6_9CAUD Lactamase_B domain-containing protein OS=Clostridium phage susfortuna OX=2316154 GN=susfortuna_gp7 PE=4 SV=1
MM1 pKa = 7.42 LKK3 pKa = 10.02 VDD5 pKa = 3.28 YY6 pKa = 10.23 HH7 pKa = 7.67 LNIEE11 pKa = 4.36 VTQGDD16 pKa = 4.05 SGCLFFGSNPYY27 pKa = 10.72 SFIGSEE33 pKa = 4.17 EE34 pKa = 3.88 LRR36 pKa = 11.84 LEE38 pKa = 4.32 LFLGDD43 pKa = 4.61 EE44 pKa = 4.88 IIFSKK49 pKa = 10.53 KK50 pKa = 10.39 SNIVDD55 pKa = 3.45 RR56 pKa = 11.84 GLLFLEE62 pKa = 4.54 LTEE65 pKa = 4.81 QEE67 pKa = 4.55 TLNLPKK73 pKa = 10.5 GIYY76 pKa = 9.64 DD77 pKa = 3.28 YY78 pKa = 11.09 RR79 pKa = 11.84 VFMTYY84 pKa = 10.79 DD85 pKa = 3.75 DD86 pKa = 4.53 NSMSEE91 pKa = 4.1 YY92 pKa = 10.66 VVNSKK97 pKa = 10.07 QFTVKK102 pKa = 10.44 GVV104 pKa = 3.01
Molecular weight: 11.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.471
IPC2_protein 4.558
IPC_protein 4.418
Toseland 4.253
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.24
Rodwell 4.253
Grimsley 4.164
Solomon 4.355
Lehninger 4.317
Nozaki 4.482
DTASelect 4.622
Thurlkill 4.266
EMBOSS 4.253
Sillero 4.52
Patrickios 3.528
IPC_peptide 4.368
IPC2_peptide 4.52
IPC2.peptide.svr19 4.472
Protein with the highest isoelectric point:
>tr|A0A385IRU9|A0A385IRU9_9CAUD Uncharacterized protein OS=Clostridium phage susfortuna OX=2316154 GN=susfortuna_gp1 PE=4 SV=1
MM1 pKa = 7.51 IKK3 pKa = 10.63 YY4 pKa = 10.02 IILWILFNLFVLIFLYY20 pKa = 10.45 DD21 pKa = 3.45 RR22 pKa = 11.84 KK23 pKa = 10.44 VRR25 pKa = 11.84 KK26 pKa = 9.23
Molecular weight: 3.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.223
IPC2_protein 9.838
IPC_protein 10.101
Toseland 10.335
ProMoST 10.145
Dawson 10.511
Bjellqvist 10.16
Wikipedia 10.672
Rodwell 11.008
Grimsley 10.57
Solomon 10.555
Lehninger 10.526
Nozaki 10.292
DTASelect 10.175
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.409
Patrickios 10.906
IPC_peptide 10.555
IPC2_peptide 8.829
IPC2.peptide.svr19 8.668
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
5907
26
799
211.0
24.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.707 ± 0.432
1.422 ± 0.198
6.247 ± 0.247
8.38 ± 0.595
4.74 ± 0.454
6.789 ± 0.832
1.473 ± 0.126
7.991 ± 0.348
9.226 ± 0.602
8.228 ± 0.45
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.174
7.686 ± 0.42
2.963 ± 0.451
3.149 ± 0.254
3.894 ± 0.286
5.113 ± 0.489
4.689 ± 0.38
5.604 ± 0.302
1.067 ± 0.183
5.383 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here