Streptococcus phage CHPC1083

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FAT7|A0A3G8FAT7_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1083 OX=2365024 GN=CHPC1083_0019 PE=4 SV=1
MM1 pKa = 7.56SVPQSIVNWFVIHH14 pKa = 7.14RR15 pKa = 11.84NLLTYY20 pKa = 11.39SMFGSRR26 pKa = 11.84NGSDD30 pKa = 3.03GTADD34 pKa = 3.56CSGSMSQALKK44 pKa = 10.76DD45 pKa = 3.46AGIPIQGLPSTVTLGQQLAKK65 pKa = 10.58NGFYY69 pKa = 10.18RR70 pKa = 11.84VSINQDD76 pKa = 2.24WDD78 pKa = 3.21ASTGDD83 pKa = 3.44IVMMSWGADD92 pKa = 3.23MSQSGGAGGHH102 pKa = 5.81VGVMMDD108 pKa = 3.26SVNFISCDD116 pKa = 3.3YY117 pKa = 8.82STQGAVGQAINTYY130 pKa = 8.91PWNDD134 pKa = 3.08YY135 pKa = 10.16YY136 pKa = 11.52AANKK140 pKa = 9.07PNYY143 pKa = 9.09IEE145 pKa = 3.75VWRR148 pKa = 11.84YY149 pKa = 10.19AEE151 pKa = 5.22SAPQTNNQANTAVVPQQKK169 pKa = 10.42AYY171 pKa = 11.13YY172 pKa = 8.27EE173 pKa = 4.42ANDD176 pKa = 3.44VQFVNGIWQIKK187 pKa = 9.47CDD189 pKa = 4.14YY190 pKa = 9.88LCPIGFNYY198 pKa = 8.74FQNGVPVTMVNWVDD212 pKa = 3.69KK213 pKa = 11.03DD214 pKa = 4.24GNDD217 pKa = 3.97LPDD220 pKa = 4.26GADD223 pKa = 3.29QEE225 pKa = 5.13FKK227 pKa = 11.28AGMFFSFAGDD237 pKa = 3.56EE238 pKa = 4.15NNITDD243 pKa = 3.82TGEE246 pKa = 3.65GGYY249 pKa = 10.61YY250 pKa = 9.76GGYY253 pKa = 8.0YY254 pKa = 9.41YY255 pKa = 10.76RR256 pKa = 11.84RR257 pKa = 11.84FEE259 pKa = 4.06FGQFGTVWLSCWNKK273 pKa = 10.66DD274 pKa = 3.89DD275 pKa = 5.14LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FAP5|A0A3G8FAP5_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1083 OX=2365024 GN=CHPC1083_0032 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.49EE18 pKa = 3.53MKK20 pKa = 9.92LYY22 pKa = 10.32QKK24 pKa = 10.55AYY26 pKa = 9.84KK27 pKa = 10.03KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.36IYY38 pKa = 9.68QLAYY42 pKa = 10.87LNRR45 pKa = 11.84LANATTKK52 pKa = 10.62DD53 pKa = 3.03GKK55 pKa = 10.64KK56 pKa = 10.46YY57 pKa = 10.49YY58 pKa = 10.19FEE60 pKa = 5.48KK61 pKa = 10.83FDD63 pKa = 3.95DD64 pKa = 4.49FYY66 pKa = 11.56NAKK69 pKa = 9.54EE70 pKa = 3.83RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.84VLGEE78 pKa = 4.35KK79 pKa = 8.61ITNSKK84 pKa = 10.18LLEE87 pKa = 3.98RR88 pKa = 11.84ARR90 pKa = 11.84NNLNYY95 pKa = 10.06KK96 pKa = 9.85KK97 pKa = 10.46EE98 pKa = 3.94RR99 pKa = 11.84GLLDD103 pKa = 3.22GRR105 pKa = 4.46

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10987

41

1545

244.2

27.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.709 ± 1.191

0.519 ± 0.108

6.189 ± 0.515

6.681 ± 0.834

4.187 ± 0.166

6.799 ± 0.545

1.329 ± 0.197

6.863 ± 0.401

7.955 ± 0.671

7.664 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.296

5.925 ± 0.466

2.849 ± 0.275

3.95 ± 0.248

4.169 ± 0.425

6.872 ± 0.677

6.435 ± 0.384

6.635 ± 0.272

1.156 ± 0.188

3.795 ± 0.496
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski