Streptomyces sp. ADI93-02

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6DE50|A0A3N6DE50_9ACTN Virulence regulon transcriptional activator VirF OS=Streptomyces sp. ADI93-02 OX=1522757 GN=virF3 PE=4 SV=1
MM1 pKa = 6.86NTGPLRR7 pKa = 11.84VSVDD11 pKa = 3.09TDD13 pKa = 3.42ACCAMGRR20 pKa = 11.84CADD23 pKa = 3.68TEE25 pKa = 4.15PRR27 pKa = 11.84VFDD30 pKa = 3.53QDD32 pKa = 3.33EE33 pKa = 4.26DD34 pKa = 3.96TGTVVLLQAAPPPDD48 pKa = 3.23LHH50 pKa = 6.75DD51 pKa = 3.68TVRR54 pKa = 11.84LCAEE58 pKa = 4.98LCPCTAITVTEE69 pKa = 4.17EE70 pKa = 3.64

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6E6K0|A0A3N6E6K0_9ACTN Putative L D-transpeptidase LppS OS=Streptomyces sp. ADI93-02 OX=1522757 GN=lppS4 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7315

0

7315

2400191

29

13431

328.1

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.517 ± 0.037

0.759 ± 0.01

5.983 ± 0.024

5.728 ± 0.028

2.739 ± 0.014

9.651 ± 0.027

2.279 ± 0.013

3.179 ± 0.02

2.167 ± 0.026

10.309 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.011

1.737 ± 0.016

6.114 ± 0.028

2.734 ± 0.017

7.915 ± 0.029

5.268 ± 0.021

6.183 ± 0.022

8.391 ± 0.025

1.48 ± 0.013

2.071 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski