Firmicutes bacterium CAG:822

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5IXR6|R5IXR6_9FIRM Rubredoxin-like domain-containing protein OS=Firmicutes bacterium CAG:822 OX=1263032 GN=BN793_01327 PE=4 SV=1
MM1 pKa = 7.42NEE3 pKa = 4.23NKK5 pKa = 10.14RR6 pKa = 11.84MLIFFISVVLVIAVILVIAFWPQSDD31 pKa = 3.77TTFACGVKK39 pKa = 10.14ADD41 pKa = 3.96SDD43 pKa = 4.12YY44 pKa = 11.87SRR46 pKa = 11.84LGSVNYY52 pKa = 9.17EE53 pKa = 3.92QYY55 pKa = 10.94SCLMEE60 pKa = 4.6QDD62 pKa = 4.18DD63 pKa = 5.03KK64 pKa = 11.53IALVVSDD71 pKa = 4.89GLNDD75 pKa = 4.49DD76 pKa = 4.39EE77 pKa = 5.7KK78 pKa = 11.5KK79 pKa = 10.83SLNDD83 pKa = 3.01VAEE86 pKa = 5.02RR87 pKa = 11.84ISKK90 pKa = 10.02SIYY93 pKa = 8.36YY94 pKa = 10.63LSDD97 pKa = 3.2EE98 pKa = 5.07VSNTDD103 pKa = 4.08LNNIKK108 pKa = 10.52DD109 pKa = 3.95DD110 pKa = 4.06LKK112 pKa = 10.21TDD114 pKa = 3.41EE115 pKa = 4.55VSYY118 pKa = 11.02DD119 pKa = 3.57NPSLVIIEE127 pKa = 4.24NGKK130 pKa = 8.19VTDD133 pKa = 5.48GIDD136 pKa = 3.41KK137 pKa = 11.0NLDD140 pKa = 3.47NSDD143 pKa = 3.9DD144 pKa = 4.12VYY146 pKa = 11.87DD147 pKa = 4.41FLQDD151 pKa = 2.94AGFAKK156 pKa = 9.96FACNATSDD164 pKa = 3.95SEE166 pKa = 4.47YY167 pKa = 10.9EE168 pKa = 3.9NLSRR172 pKa = 11.84LTYY175 pKa = 10.32DD176 pKa = 4.28QYY178 pKa = 11.78QCLYY182 pKa = 10.9DD183 pKa = 3.65SGEE186 pKa = 4.18PFVMILTQSTCSYY199 pKa = 9.89CAQFLPVINEE209 pKa = 3.99YY210 pKa = 10.82AGEE213 pKa = 4.2NNLPVYY219 pKa = 10.12FLEE222 pKa = 5.67IDD224 pKa = 3.56TMDD227 pKa = 3.99SDD229 pKa = 4.96DD230 pKa = 4.13LQSVFSSLSYY240 pKa = 10.85FSDD243 pKa = 3.26NSDD246 pKa = 2.57WGTPLTLGIKK256 pKa = 10.01DD257 pKa = 3.67KK258 pKa = 11.3EE259 pKa = 4.62VVTEE263 pKa = 4.01LSGYY267 pKa = 8.99TDD269 pKa = 3.55DD270 pKa = 4.19TSSIEE275 pKa = 4.04NLFTEE280 pKa = 5.36LGLKK284 pKa = 9.65

Molecular weight: 31.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5IVW1|R5IVW1_9FIRM Methyltransferase putative OS=Firmicutes bacterium CAG:822 OX=1263032 GN=BN793_00693 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84KK12 pKa = 9.8RR13 pKa = 11.84SKK15 pKa = 11.13KK16 pKa = 8.1MGFFARR22 pKa = 11.84MKK24 pKa = 10.39AGIVSRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 9.75KK34 pKa = 9.35GRR36 pKa = 11.84KK37 pKa = 8.41VLSHH41 pKa = 6.79

Molecular weight: 4.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1377

0

1377

397830

29

1853

288.9

32.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.781 ± 0.067

0.994 ± 0.022

6.339 ± 0.056

7.14 ± 0.073

4.321 ± 0.064

5.629 ± 0.065

1.437 ± 0.031

9.427 ± 0.086

9.622 ± 0.092

9.144 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.026

7.026 ± 0.072

2.641 ± 0.036

2.3 ± 0.037

3.095 ± 0.045

6.122 ± 0.086

5.643 ± 0.103

6.19 ± 0.062

0.611 ± 0.02

5.066 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski