Sunxiuqinia elliptica
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4090 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2D559|A0A1I2D559_9BACT FecR family protein OS=Sunxiuqinia elliptica OX=655355 GN=SAMN05216283_101927 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.36 KK3 pKa = 10.2 QILTLTLFVLAIFAGSYY20 pKa = 10.22 SVFGQATVLNQAITASQPIPALSCVAGSEE49 pKa = 4.14 PLHH52 pKa = 6.57 PYY54 pKa = 9.48 PGQSYY59 pKa = 8.09 TYY61 pKa = 10.2 TMDD64 pKa = 3.5 GSSGAEE70 pKa = 4.08 TADD73 pKa = 2.86 EE74 pKa = 4.23 WTWFVTKK81 pKa = 10.59 DD82 pKa = 3.46 PEE84 pKa = 4.71 FINATGLNTANMLTTASGDD103 pKa = 3.71 LLNVGTNYY111 pKa = 8.41 GTATAGANSIDD122 pKa = 3.79 ITWSPEE128 pKa = 3.52 VLANTLYY135 pKa = 10.66 QGTPGTDD142 pKa = 3.35 PSPTFVVGYY151 pKa = 8.47 ATGTNCADD159 pKa = 3.82 NIQVYY164 pKa = 9.58 EE165 pKa = 4.34 INPKK169 pKa = 10.28 FNFTIDD175 pKa = 3.03 IANVDD180 pKa = 4.22 TVNSTLGWDD189 pKa = 3.46 AAFSQCVDD197 pKa = 3.06 IVRR200 pKa = 11.84 SATYY204 pKa = 10.76 NSTTKK209 pKa = 10.55 EE210 pKa = 3.25 IDD212 pKa = 3.17 VNYY215 pKa = 10.5 GKK217 pKa = 10.37 DD218 pKa = 2.89 ILYY221 pKa = 10.75 FEE223 pKa = 4.4 VAAANFVTDD232 pKa = 3.93 FTPTFHH238 pKa = 7.37 LISGLVGTQTAVVTLHH254 pKa = 7.0 PSLADD259 pKa = 3.41 AKK261 pKa = 10.98 AGTNVAATANWDD273 pKa = 3.58 ATSVGTDD280 pKa = 2.81 WATGVQFTSSDD291 pKa = 3.5 PALVVSGVSLFVKK304 pKa = 9.84 VVISNNTEE312 pKa = 3.44 EE313 pKa = 4.76 SLTDD317 pKa = 3.78 NIFALAVDD325 pKa = 4.04 AQDD328 pKa = 4.08 NNQTGIWDD336 pKa = 4.2 MEE338 pKa = 5.12 DD339 pKa = 4.14 DD340 pKa = 4.56 DD341 pKa = 5.08 CTTATDD347 pKa = 4.18 AADD350 pKa = 3.35 QADD353 pKa = 4.2 FAEE356 pKa = 4.72 HH357 pKa = 6.07 TVTPRR362 pKa = 11.84 PQIDD366 pKa = 3.77 HH367 pKa = 6.61 ATPDD371 pKa = 3.51 AGAPDD376 pKa = 4.35 PTTVITKK383 pKa = 8.64 TPP385 pKa = 3.31
Molecular weight: 40.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.897
Patrickios 0.757
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A1I2ASV1|A0A1I2ASV1_9BACT Sporulation related domain-containing protein OS=Sunxiuqinia elliptica OX=655355 GN=SAMN05216283_101215 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 8.89 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 10.06 HH16 pKa = 3.64 GFRR19 pKa = 11.84 AKK21 pKa = 9.35 MASTSGRR28 pKa = 11.84 KK29 pKa = 8.27 VLKK32 pKa = 10.26 LRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.12 GRR39 pKa = 11.84 KK40 pKa = 8.74 KK41 pKa = 10.01 LTVSDD46 pKa = 4.02 EE47 pKa = 4.2 PRR49 pKa = 11.84 HH50 pKa = 5.57 KK51 pKa = 10.63 RR52 pKa = 3.34
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.939
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.061
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4090
0
4090
1510668
29
5174
369.4
41.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.889 ± 0.035
0.823 ± 0.013
5.526 ± 0.028
6.852 ± 0.035
5.002 ± 0.026
6.819 ± 0.037
1.926 ± 0.019
6.87 ± 0.037
6.619 ± 0.037
9.674 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.328 ± 0.017
5.207 ± 0.033
3.765 ± 0.023
4.095 ± 0.031
4.152 ± 0.027
6.373 ± 0.031
5.235 ± 0.043
6.46 ± 0.032
1.341 ± 0.016
4.041 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here