Amino acid dipepetide frequency for Staphylococcus phage vB_SauS_phi2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.667AlaAla: 2.667 ± 0.748
0.288AlaCys: 0.288 ± 0.15
2.955AlaAsp: 2.955 ± 0.465
4.541AlaGlu: 4.541 ± 0.505
1.874AlaPhe: 1.874 ± 0.312
3.316AlaGly: 3.316 ± 0.654
1.297AlaHis: 1.297 ± 0.285
4.902AlaIle: 4.902 ± 0.747
6.055AlaLys: 6.055 ± 1.011
4.974AlaLeu: 4.974 ± 0.75
1.37AlaMet: 1.37 ± 0.285
4.181AlaAsn: 4.181 ± 0.878
1.514AlaPro: 1.514 ± 0.351
1.802AlaGln: 1.802 ± 0.391
3.027AlaArg: 3.027 ± 0.382
5.262AlaSer: 5.262 ± 0.752
2.955AlaThr: 2.955 ± 0.55
2.667AlaVal: 2.667 ± 0.574
1.225AlaTrp: 1.225 ± 0.348
2.811AlaTyr: 2.811 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
0.216CysAla: 0.216 ± 0.117
0.0CysCys: 0.0 ± 0.0
0.36CysAsp: 0.36 ± 0.183
0.288CysGlu: 0.288 ± 0.187
0.36CysPhe: 0.36 ± 0.167
0.288CysGly: 0.288 ± 0.152
0.144CysHis: 0.144 ± 0.107
0.432CysIle: 0.432 ± 0.177
0.649CysLys: 0.649 ± 0.225
0.432CysLeu: 0.432 ± 0.186
0.072CysMet: 0.072 ± 0.069
0.0CysAsn: 0.0 ± 0.0
0.216CysPro: 0.216 ± 0.16
0.288CysGln: 0.288 ± 0.139
0.505CysArg: 0.505 ± 0.236
0.216CysSer: 0.216 ± 0.126
0.432CysThr: 0.432 ± 0.193
0.072CysVal: 0.072 ± 0.076
0.0CysTrp: 0.0 ± 0.0
0.36CysTyr: 0.36 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
2.739AspAla: 2.739 ± 0.578
0.216AspCys: 0.216 ± 0.132
3.82AspAsp: 3.82 ± 0.688
5.262AspGlu: 5.262 ± 0.872
3.748AspPhe: 3.748 ± 0.484
3.892AspGly: 3.892 ± 0.722
0.649AspHis: 0.649 ± 0.198
5.262AspIle: 5.262 ± 0.613
6.415AspLys: 6.415 ± 0.875
5.478AspLeu: 5.478 ± 0.564
1.946AspMet: 1.946 ± 0.383
2.955AspAsn: 2.955 ± 0.406
1.081AspPro: 1.081 ± 0.314
1.586AspGln: 1.586 ± 0.32
1.874AspArg: 1.874 ± 0.373
3.748AspSer: 3.748 ± 0.62
3.748AspThr: 3.748 ± 0.487
3.604AspVal: 3.604 ± 0.448
0.649AspTrp: 0.649 ± 0.198
3.532AspTyr: 3.532 ± 0.619
0.0AspXaa: 0.0 ± 0.0
Glu
5.334GluAla: 5.334 ± 0.567
0.721GluCys: 0.721 ± 0.297
4.974GluAsp: 4.974 ± 0.736
8.362GluGlu: 8.362 ± 1.203
2.811GluPhe: 2.811 ± 0.561
3.316GluGly: 3.316 ± 0.627
1.442GluHis: 1.442 ± 0.383
5.55GluIle: 5.55 ± 0.845
7.929GluLys: 7.929 ± 0.901
7.497GluLeu: 7.497 ± 0.918
2.451GluMet: 2.451 ± 0.489
5.046GluAsn: 5.046 ± 0.57
1.586GluPro: 1.586 ± 0.418
3.46GluGln: 3.46 ± 0.557
3.027GluArg: 3.027 ± 0.572
3.892GluSer: 3.892 ± 0.518
4.397GluThr: 4.397 ± 0.674
4.397GluVal: 4.397 ± 0.601
1.153GluTrp: 1.153 ± 0.215
3.316GluTyr: 3.316 ± 0.752
0.0GluXaa: 0.0 ± 0.0
Phe
1.946PheAla: 1.946 ± 0.411
0.432PheCys: 0.432 ± 0.162
3.604PheAsp: 3.604 ± 0.496
3.172PheGlu: 3.172 ± 0.581
0.865PhePhe: 0.865 ± 0.267
3.027PheGly: 3.027 ± 0.58
0.432PheHis: 0.432 ± 0.176
3.172PheIle: 3.172 ± 0.686
4.613PheLys: 4.613 ± 0.586
2.595PheLeu: 2.595 ± 0.515
0.937PheMet: 0.937 ± 0.241
3.532PheAsn: 3.532 ± 0.541
0.865PhePro: 0.865 ± 0.295
1.225PheGln: 1.225 ± 0.303
1.081PheArg: 1.081 ± 0.278
1.874PheSer: 1.874 ± 0.409
1.874PheThr: 1.874 ± 0.358
2.018PheVal: 2.018 ± 0.415
0.36PheTrp: 0.36 ± 0.132
1.514PheTyr: 1.514 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
4.109GlyAla: 4.109 ± 0.963
0.288GlyCys: 0.288 ± 0.143
4.037GlyAsp: 4.037 ± 0.52
3.46GlyGlu: 3.46 ± 0.43
2.451GlyPhe: 2.451 ± 0.379
5.118GlyGly: 5.118 ± 1.157
1.37GlyHis: 1.37 ± 0.315
3.82GlyIle: 3.82 ± 0.547
5.767GlyLys: 5.767 ± 0.648
5.839GlyLeu: 5.839 ± 0.976
1.37GlyMet: 1.37 ± 0.379
2.595GlyAsn: 2.595 ± 0.469
0.865GlyPro: 0.865 ± 0.232
1.514GlyGln: 1.514 ± 0.323
1.946GlyArg: 1.946 ± 0.472
3.892GlySer: 3.892 ± 0.715
4.037GlyThr: 4.037 ± 0.627
4.181GlyVal: 4.181 ± 0.767
1.153GlyTrp: 1.153 ± 0.424
2.451GlyTyr: 2.451 ± 0.586
0.0GlyXaa: 0.0 ± 0.0
His
1.009HisAla: 1.009 ± 0.236
0.216HisCys: 0.216 ± 0.122
0.649HisAsp: 0.649 ± 0.273
1.081HisGlu: 1.081 ± 0.274
0.937HisPhe: 0.937 ± 0.228
0.937HisGly: 0.937 ± 0.25
0.505HisHis: 0.505 ± 0.198
1.153HisIle: 1.153 ± 0.315
1.442HisLys: 1.442 ± 0.309
1.658HisLeu: 1.658 ± 0.29
0.288HisMet: 0.288 ± 0.146
1.153HisAsn: 1.153 ± 0.293
0.937HisPro: 0.937 ± 0.22
0.865HisGln: 0.865 ± 0.263
0.793HisArg: 0.793 ± 0.208
1.225HisSer: 1.225 ± 0.244
1.153HisThr: 1.153 ± 0.25
0.937HisVal: 0.937 ± 0.251
0.36HisTrp: 0.36 ± 0.143
1.442HisTyr: 1.442 ± 0.335
0.0HisXaa: 0.0 ± 0.0
Ile
3.82IleAla: 3.82 ± 0.572
0.432IleCys: 0.432 ± 0.187
5.767IleAsp: 5.767 ± 0.817
6.271IleGlu: 6.271 ± 0.749
2.235IlePhe: 2.235 ± 0.511
3.388IleGly: 3.388 ± 0.531
1.514IleHis: 1.514 ± 0.318
4.181IleIle: 4.181 ± 0.609
6.848IleLys: 6.848 ± 0.632
5.046IleLeu: 5.046 ± 0.73
1.658IleMet: 1.658 ± 0.369
4.397IleAsn: 4.397 ± 0.558
2.018IlePro: 2.018 ± 0.295
2.018IleGln: 2.018 ± 0.401
3.244IleArg: 3.244 ± 0.455
4.109IleSer: 4.109 ± 0.531
4.181IleThr: 4.181 ± 0.626
3.82IleVal: 3.82 ± 0.699
0.288IleTrp: 0.288 ± 0.135
2.739IleTyr: 2.739 ± 0.516
0.0IleXaa: 0.0 ± 0.0
Lys
7.28LysAla: 7.28 ± 1.168
0.144LysCys: 0.144 ± 0.098
4.83LysAsp: 4.83 ± 0.621
9.082LysGlu: 9.082 ± 0.966
2.162LysPhe: 2.162 ± 0.362
5.478LysGly: 5.478 ± 0.933
2.09LysHis: 2.09 ± 0.449
5.55LysIle: 5.55 ± 0.685
7.857LysLys: 7.857 ± 1.011
8.722LysLeu: 8.722 ± 1.09
2.883LysMet: 2.883 ± 0.489
5.55LysAsn: 5.55 ± 0.73
2.307LysPro: 2.307 ± 0.42
5.478LysGln: 5.478 ± 0.59
4.181LysArg: 4.181 ± 0.657
5.839LysSer: 5.839 ± 1.274
5.118LysThr: 5.118 ± 0.708
5.406LysVal: 5.406 ± 0.659
1.874LysTrp: 1.874 ± 0.48
4.397LysTyr: 4.397 ± 0.58
0.0LysXaa: 0.0 ± 0.0
Leu
4.757LeuAla: 4.757 ± 0.864
0.36LeuCys: 0.36 ± 0.175
5.19LeuAsp: 5.19 ± 0.929
6.56LeuGlu: 6.56 ± 0.651
3.892LeuPhe: 3.892 ± 0.584
4.253LeuGly: 4.253 ± 0.969
1.081LeuHis: 1.081 ± 0.367
5.118LeuIle: 5.118 ± 0.749
9.082LeuLys: 9.082 ± 1.068
6.487LeuLeu: 6.487 ± 0.787
1.802LeuMet: 1.802 ± 0.293
5.406LeuAsn: 5.406 ± 0.543
3.027LeuPro: 3.027 ± 0.548
2.883LeuGln: 2.883 ± 0.63
4.037LeuArg: 4.037 ± 0.555
5.839LeuSer: 5.839 ± 0.756
5.046LeuThr: 5.046 ± 0.614
3.748LeuVal: 3.748 ± 0.423
0.505LeuTrp: 0.505 ± 0.195
3.388LeuTyr: 3.388 ± 0.766
0.0LeuXaa: 0.0 ± 0.0
Met
1.297MetAla: 1.297 ± 0.301
0.216MetCys: 0.216 ± 0.12
0.937MetAsp: 0.937 ± 0.387
1.946MetGlu: 1.946 ± 0.447
0.793MetPhe: 0.793 ± 0.278
1.73MetGly: 1.73 ± 0.539
0.649MetHis: 0.649 ± 0.233
1.442MetIle: 1.442 ± 0.237
2.667MetLys: 2.667 ± 0.533
1.874MetLeu: 1.874 ± 0.446
0.505MetMet: 0.505 ± 0.211
1.73MetAsn: 1.73 ± 0.397
1.009MetPro: 1.009 ± 0.226
1.442MetGln: 1.442 ± 0.399
1.225MetArg: 1.225 ± 0.297
1.946MetSer: 1.946 ± 0.45
2.307MetThr: 2.307 ± 0.329
1.153MetVal: 1.153 ± 0.213
0.288MetTrp: 0.288 ± 0.136
0.721MetTyr: 0.721 ± 0.248
0.0MetXaa: 0.0 ± 0.0
Asn
4.109AsnAla: 4.109 ± 0.456
0.144AsnCys: 0.144 ± 0.102
3.532AsnAsp: 3.532 ± 0.602
4.902AsnGlu: 4.902 ± 0.847
1.73AsnPhe: 1.73 ± 0.295
4.397AsnGly: 4.397 ± 0.668
1.153AsnHis: 1.153 ± 0.293
3.82AsnIle: 3.82 ± 0.478
6.487AsnLys: 6.487 ± 0.545
4.902AsnLeu: 4.902 ± 0.656
1.081AsnMet: 1.081 ± 0.229
3.1AsnAsn: 3.1 ± 0.613
2.307AsnPro: 2.307 ± 0.369
2.451AsnGln: 2.451 ± 0.385
2.883AsnArg: 2.883 ± 0.424
3.82AsnSer: 3.82 ± 0.643
4.037AsnThr: 4.037 ± 0.495
2.667AsnVal: 2.667 ± 0.457
1.297AsnTrp: 1.297 ± 0.309
2.739AsnTyr: 2.739 ± 0.461
0.0AsnXaa: 0.0 ± 0.0
Pro
1.297ProAla: 1.297 ± 0.307
0.216ProCys: 0.216 ± 0.133
1.297ProAsp: 1.297 ± 0.324
2.162ProGlu: 2.162 ± 0.473
1.586ProPhe: 1.586 ± 0.422
2.09ProGly: 2.09 ± 0.371
0.432ProHis: 0.432 ± 0.193
1.586ProIle: 1.586 ± 0.334
2.451ProLys: 2.451 ± 0.501
2.307ProLeu: 2.307 ± 0.509
0.649ProMet: 0.649 ± 0.246
1.946ProAsn: 1.946 ± 0.375
0.505ProPro: 0.505 ± 0.222
1.153ProGln: 1.153 ± 0.3
1.297ProArg: 1.297 ± 0.293
1.946ProSer: 1.946 ± 0.359
1.658ProThr: 1.658 ± 0.336
1.442ProVal: 1.442 ± 0.391
0.36ProTrp: 0.36 ± 0.145
1.009ProTyr: 1.009 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
3.027GlnAla: 3.027 ± 0.454
0.144GlnCys: 0.144 ± 0.093
2.09GlnAsp: 2.09 ± 0.374
2.451GlnGlu: 2.451 ± 0.473
1.297GlnPhe: 1.297 ± 0.265
2.09GlnGly: 2.09 ± 0.46
0.793GlnHis: 0.793 ± 0.172
3.027GlnIle: 3.027 ± 0.593
3.172GlnLys: 3.172 ± 0.515
3.532GlnLeu: 3.532 ± 0.537
1.009GlnMet: 1.009 ± 0.344
2.09GlnAsn: 2.09 ± 0.435
1.586GlnPro: 1.586 ± 0.43
1.225GlnGln: 1.225 ± 0.398
2.162GlnArg: 2.162 ± 0.391
2.235GlnSer: 2.235 ± 0.372
1.37GlnThr: 1.37 ± 0.363
2.523GlnVal: 2.523 ± 0.371
0.505GlnTrp: 0.505 ± 0.213
1.802GlnTyr: 1.802 ± 0.3
0.0GlnXaa: 0.0 ± 0.0
Arg
2.595ArgAla: 2.595 ± 0.502
0.072ArgCys: 0.072 ± 0.075
2.883ArgAsp: 2.883 ± 0.36
2.595ArgGlu: 2.595 ± 0.428
2.595ArgPhe: 2.595 ± 0.374
1.946ArgGly: 1.946 ± 0.475
1.009ArgHis: 1.009 ± 0.265
3.316ArgIle: 3.316 ± 0.576
4.253ArgLys: 4.253 ± 0.537
3.532ArgLeu: 3.532 ± 0.684
1.153ArgMet: 1.153 ± 0.304
2.883ArgAsn: 2.883 ± 0.44
0.937ArgPro: 0.937 ± 0.37
1.514ArgGln: 1.514 ± 0.294
1.802ArgArg: 1.802 ± 0.387
2.018ArgSer: 2.018 ± 0.399
2.307ArgThr: 2.307 ± 0.403
1.946ArgVal: 1.946 ± 0.341
0.288ArgTrp: 0.288 ± 0.147
2.09ArgTyr: 2.09 ± 0.455
0.0ArgXaa: 0.0 ± 0.0
Ser
4.109SerAla: 4.109 ± 0.795
0.505SerCys: 0.505 ± 0.223
4.613SerAsp: 4.613 ± 0.511
4.469SerGlu: 4.469 ± 0.484
2.162SerPhe: 2.162 ± 0.451
4.181SerGly: 4.181 ± 0.895
0.937SerHis: 0.937 ± 0.318
3.676SerIle: 3.676 ± 0.424
6.487SerLys: 6.487 ± 1.223
4.613SerLeu: 4.613 ± 0.601
2.235SerMet: 2.235 ± 0.316
5.19SerAsn: 5.19 ± 0.55
1.73SerPro: 1.73 ± 0.438
2.451SerGln: 2.451 ± 0.411
2.09SerArg: 2.09 ± 0.416
4.181SerSer: 4.181 ± 0.818
3.172SerThr: 3.172 ± 0.48
4.181SerVal: 4.181 ± 0.576
0.865SerTrp: 0.865 ± 0.229
1.946SerTyr: 1.946 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
3.82ThrAla: 3.82 ± 0.489
0.216ThrCys: 0.216 ± 0.143
3.748ThrAsp: 3.748 ± 0.512
4.469ThrGlu: 4.469 ± 0.522
2.883ThrPhe: 2.883 ± 0.389
4.181ThrGly: 4.181 ± 0.58
1.37ThrHis: 1.37 ± 0.326
4.613ThrIle: 4.613 ± 0.658
5.118ThrLys: 5.118 ± 0.604
3.892ThrLeu: 3.892 ± 0.568
0.865ThrMet: 0.865 ± 0.275
2.883ThrAsn: 2.883 ± 0.416
1.802ThrPro: 1.802 ± 0.258
1.658ThrGln: 1.658 ± 0.278
1.586ThrArg: 1.586 ± 0.279
3.532ThrSer: 3.532 ± 0.531
2.811ThrThr: 2.811 ± 0.432
4.253ThrVal: 4.253 ± 0.502
0.721ThrTrp: 0.721 ± 0.246
2.235ThrTyr: 2.235 ± 0.413
0.0ThrXaa: 0.0 ± 0.0
Val
3.027ValAla: 3.027 ± 0.395
0.216ValCys: 0.216 ± 0.122
3.748ValAsp: 3.748 ± 0.615
5.046ValGlu: 5.046 ± 0.692
2.09ValPhe: 2.09 ± 0.409
3.604ValGly: 3.604 ± 0.603
0.865ValHis: 0.865 ± 0.237
4.037ValIle: 4.037 ± 0.453
4.469ValLys: 4.469 ± 0.579
4.397ValLeu: 4.397 ± 0.542
1.37ValMet: 1.37 ± 0.283
3.676ValAsn: 3.676 ± 0.478
1.73ValPro: 1.73 ± 0.397
2.162ValGln: 2.162 ± 0.312
2.667ValArg: 2.667 ± 0.421
3.965ValSer: 3.965 ± 0.539
2.955ValThr: 2.955 ± 0.561
2.883ValVal: 2.883 ± 0.488
0.721ValTrp: 0.721 ± 0.231
2.307ValTyr: 2.307 ± 0.484
0.0ValXaa: 0.0 ± 0.0
Trp
0.432TrpAla: 0.432 ± 0.161
0.0TrpCys: 0.0 ± 0.0
0.36TrpAsp: 0.36 ± 0.185
1.153TrpGlu: 1.153 ± 0.373
1.153TrpPhe: 1.153 ± 0.361
0.36TrpGly: 0.36 ± 0.176
0.072TrpHis: 0.072 ± 0.068
0.865TrpIle: 0.865 ± 0.298
0.793TrpLys: 0.793 ± 0.237
1.153TrpLeu: 1.153 ± 0.265
0.505TrpMet: 0.505 ± 0.222
0.937TrpAsn: 0.937 ± 0.239
0.288TrpPro: 0.288 ± 0.192
0.721TrpGln: 0.721 ± 0.198
0.577TrpArg: 0.577 ± 0.192
1.225TrpSer: 1.225 ± 0.387
0.577TrpThr: 0.577 ± 0.173
1.225TrpVal: 1.225 ± 0.312
0.144TrpTrp: 0.144 ± 0.122
0.649TrpTyr: 0.649 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.09TyrAla: 2.09 ± 0.312
0.505TyrCys: 0.505 ± 0.183
2.883TyrAsp: 2.883 ± 0.501
3.388TyrGlu: 3.388 ± 0.724
1.514TyrPhe: 1.514 ± 0.322
2.667TyrGly: 2.667 ± 0.642
0.937TyrHis: 0.937 ± 0.3
2.451TyrIle: 2.451 ± 0.532
3.676TyrLys: 3.676 ± 0.512
3.532TyrLeu: 3.532 ± 0.659
1.658TyrMet: 1.658 ± 0.291
2.307TyrAsn: 2.307 ± 0.512
1.081TyrPro: 1.081 ± 0.322
2.235TyrGln: 2.235 ± 0.424
1.73TyrArg: 1.73 ± 0.49
3.027TyrSer: 3.027 ± 0.466
2.523TyrThr: 2.523 ± 0.485
2.811TyrVal: 2.811 ± 0.5
0.432TyrTrp: 0.432 ± 0.139
1.586TyrTyr: 1.586 ± 0.473
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (13874 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski