Apricot latent virus
Average proteome isoelectric point is 7.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7DUC1|E7DUC1_9VIRU Movement protein TGB2 OS=Apricot latent virus OX=75387 GN=tgb2 PE=3 SV=1
MM1 pKa = 6.96 EE2 pKa = 4.71 TVLSLLNEE10 pKa = 4.49 FGFEE14 pKa = 3.91 RR15 pKa = 11.84 TVEE18 pKa = 4.05 PLSDD22 pKa = 4.94 PIVVHH27 pKa = 6.55 AVPGSGKK34 pKa = 7.26 TTLIKK39 pKa = 10.2 QALIRR44 pKa = 11.84 NHH46 pKa = 6.45 NIEE49 pKa = 4.16 AVTFGVPEE57 pKa = 3.99 KK58 pKa = 11.17 ANIHH62 pKa = 4.48 GTYY65 pKa = 9.33 IKK67 pKa = 10.43 KK68 pKa = 10.18 ARR70 pKa = 11.84 QGQRR74 pKa = 11.84 GRR76 pKa = 11.84 GNFSILDD83 pKa = 3.72 EE84 pKa = 4.39 YY85 pKa = 11.62 LSGEE89 pKa = 4.15 YY90 pKa = 9.06 STGFNCLFSDD100 pKa = 5.21 PYY102 pKa = 10.8 QNHH105 pKa = 6.32 GDD107 pKa = 3.82 CLRR110 pKa = 11.84 AHH112 pKa = 7.05 FIGRR116 pKa = 11.84 CSHH119 pKa = 6.71 RR120 pKa = 11.84 FGNQTVQVLRR130 pKa = 11.84 NLGYY134 pKa = 10.82 NIASSKK140 pKa = 10.51 EE141 pKa = 3.9 DD142 pKa = 3.1 IVEE145 pKa = 3.97 KK146 pKa = 10.94 KK147 pKa = 10.65 NIFRR151 pKa = 11.84 LVEE154 pKa = 3.8 PEE156 pKa = 3.79 GAIICLEE163 pKa = 4.02 KK164 pKa = 10.67 EE165 pKa = 4.4 VEE167 pKa = 5.46 DD168 pKa = 4.33 FLAWHH173 pKa = 5.93 HH174 pKa = 6.33 VEE176 pKa = 4.81 YY177 pKa = 10.9 KK178 pKa = 10.62 LPCQVRR184 pKa = 11.84 GATFDD189 pKa = 3.38 TVTFIHH195 pKa = 6.59 EE196 pKa = 4.4 KK197 pKa = 10.01 PLEE200 pKa = 4.11 EE201 pKa = 4.61 LVGPDD206 pKa = 2.87 WYY208 pKa = 11.19 VALTRR213 pKa = 11.84 HH214 pKa = 4.85 RR215 pKa = 11.84 HH216 pKa = 4.78 KK217 pKa = 10.97 LVLVSNN223 pKa = 4.93
Molecular weight: 25.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.242
IPC2_protein 6.16
IPC_protein 6.287
Toseland 6.62
ProMoST 6.678
Dawson 6.649
Bjellqvist 6.59
Wikipedia 6.664
Rodwell 6.634
Grimsley 6.766
Solomon 6.649
Lehninger 6.649
Nozaki 6.912
DTASelect 7.088
Thurlkill 7.117
EMBOSS 7.102
Sillero 7.029
Patrickios 4.202
IPC_peptide 6.664
IPC2_peptide 6.927
IPC2.peptide.svr19 6.836
Protein with the highest isoelectric point:
>tr|E7DUC2|E7DUC2_9VIRU Movement protein TGBp3 OS=Apricot latent virus OX=75387 GN=tgb3 PE=3 SV=1
MM1 pKa = 7.81 PFSQPPDD8 pKa = 3.35 YY9 pKa = 10.55 SKK11 pKa = 11.33 SVFPIAIGAAVALVLFTLTRR31 pKa = 11.84 STLPQVGDD39 pKa = 3.89 NIHH42 pKa = 6.29 NLPHH46 pKa = 6.56 GGNYY50 pKa = 9.26 QDD52 pKa = 3.31 GTKK55 pKa = 10.06 RR56 pKa = 11.84 ISYY59 pKa = 9.1 CGPKK63 pKa = 10.24 NSFPSSSLISSGTPMIIIVILLTAAIYY90 pKa = 10.57 VSEE93 pKa = 4.1 RR94 pKa = 11.84 WFGSGHH100 pKa = 5.85 RR101 pKa = 11.84 RR102 pKa = 11.84 CSCCLPGAPACTATNHH118 pKa = 5.39 EE119 pKa = 4.41
Molecular weight: 12.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.346
IPC2_protein 7.702
IPC_protein 7.688
Toseland 7.205
ProMoST 8.068
Dawson 8.273
Bjellqvist 8.712
Wikipedia 8.185
Rodwell 8.258
Grimsley 7.19
Solomon 8.419
Lehninger 8.448
Nozaki 8.975
DTASelect 8.39
Thurlkill 8.419
EMBOSS 8.492
Sillero 8.785
Patrickios 2.27
IPC_peptide 8.419
IPC2_peptide 8.258
IPC2.peptide.svr19 8.212
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3017
70
2184
603.4
67.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.497 ± 1.242
2.353 ± 0.505
5.204 ± 1.425
6.364 ± 0.96
6.032 ± 1.065
6.364 ± 0.992
2.95 ± 0.602
5.867 ± 0.694
6.198 ± 1.489
9.546 ± 1.382
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.922 ± 0.262
4.276 ± 0.422
4.508 ± 1.353
3.016 ± 0.79
5.171 ± 0.333
8.286 ± 0.771
5.568 ± 1.674
6.397 ± 1.136
0.895 ± 0.19
2.585 ± 0.623
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here