Apricot latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E7DUC1|E7DUC1_9VIRU Movement protein TGB2 OS=Apricot latent virus OX=75387 GN=tgb2 PE=3 SV=1
MM1 pKa = 6.96EE2 pKa = 4.71TVLSLLNEE10 pKa = 4.49FGFEE14 pKa = 3.91RR15 pKa = 11.84TVEE18 pKa = 4.05PLSDD22 pKa = 4.94PIVVHH27 pKa = 6.55AVPGSGKK34 pKa = 7.26TTLIKK39 pKa = 10.2QALIRR44 pKa = 11.84NHH46 pKa = 6.45NIEE49 pKa = 4.16AVTFGVPEE57 pKa = 3.99KK58 pKa = 11.17ANIHH62 pKa = 4.48GTYY65 pKa = 9.33IKK67 pKa = 10.43KK68 pKa = 10.18ARR70 pKa = 11.84QGQRR74 pKa = 11.84GRR76 pKa = 11.84GNFSILDD83 pKa = 3.72EE84 pKa = 4.39YY85 pKa = 11.62LSGEE89 pKa = 4.15YY90 pKa = 9.06STGFNCLFSDD100 pKa = 5.21PYY102 pKa = 10.8QNHH105 pKa = 6.32GDD107 pKa = 3.82CLRR110 pKa = 11.84AHH112 pKa = 7.05FIGRR116 pKa = 11.84CSHH119 pKa = 6.71RR120 pKa = 11.84FGNQTVQVLRR130 pKa = 11.84NLGYY134 pKa = 10.82NIASSKK140 pKa = 10.51EE141 pKa = 3.9DD142 pKa = 3.1IVEE145 pKa = 3.97KK146 pKa = 10.94KK147 pKa = 10.65NIFRR151 pKa = 11.84LVEE154 pKa = 3.8PEE156 pKa = 3.79GAIICLEE163 pKa = 4.02KK164 pKa = 10.67EE165 pKa = 4.4VEE167 pKa = 5.46DD168 pKa = 4.33FLAWHH173 pKa = 5.93HH174 pKa = 6.33VEE176 pKa = 4.81YY177 pKa = 10.9KK178 pKa = 10.62LPCQVRR184 pKa = 11.84GATFDD189 pKa = 3.38TVTFIHH195 pKa = 6.59EE196 pKa = 4.4KK197 pKa = 10.01PLEE200 pKa = 4.11EE201 pKa = 4.61LVGPDD206 pKa = 2.87WYY208 pKa = 11.19VALTRR213 pKa = 11.84HH214 pKa = 4.85RR215 pKa = 11.84HH216 pKa = 4.78KK217 pKa = 10.97LVLVSNN223 pKa = 4.93

Molecular weight:
25.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E7DUC2|E7DUC2_9VIRU Movement protein TGBp3 OS=Apricot latent virus OX=75387 GN=tgb3 PE=3 SV=1
MM1 pKa = 7.81PFSQPPDD8 pKa = 3.35YY9 pKa = 10.55SKK11 pKa = 11.33SVFPIAIGAAVALVLFTLTRR31 pKa = 11.84STLPQVGDD39 pKa = 3.89NIHH42 pKa = 6.29NLPHH46 pKa = 6.56GGNYY50 pKa = 9.26QDD52 pKa = 3.31GTKK55 pKa = 10.06RR56 pKa = 11.84ISYY59 pKa = 9.1CGPKK63 pKa = 10.24NSFPSSSLISSGTPMIIIVILLTAAIYY90 pKa = 10.57VSEE93 pKa = 4.1RR94 pKa = 11.84WFGSGHH100 pKa = 5.85RR101 pKa = 11.84RR102 pKa = 11.84CSCCLPGAPACTATNHH118 pKa = 5.39EE119 pKa = 4.41

Molecular weight:
12.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3017

70

2184

603.4

67.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.497 ± 1.242

2.353 ± 0.505

5.204 ± 1.425

6.364 ± 0.96

6.032 ± 1.065

6.364 ± 0.992

2.95 ± 0.602

5.867 ± 0.694

6.198 ± 1.489

9.546 ± 1.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.922 ± 0.262

4.276 ± 0.422

4.508 ± 1.353

3.016 ± 0.79

5.171 ± 0.333

8.286 ± 0.771

5.568 ± 1.674

6.397 ± 1.136

0.895 ± 0.19

2.585 ± 0.623

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski