Bolahun virus variant 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Xinmoviridae; Anphevirus; Bolahun anphevirus; Bolahun virus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9U5D8|A0A1C9U5D8_9MONO Putative small ZnF protein OS=Bolahun virus variant 2 OX=1903560 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 4.07MALQQGNEE10 pKa = 4.15YY11 pKa = 10.33YY12 pKa = 10.18ISDD15 pKa = 4.68DD16 pKa = 4.62DD17 pKa = 5.03SDD19 pKa = 4.04GGPDD23 pKa = 3.49LFIPISQVDD32 pKa = 3.77TLRR35 pKa = 11.84EE36 pKa = 3.91MGFLPVSYY44 pKa = 10.19GHH46 pKa = 5.98YY47 pKa = 10.6SKK49 pKa = 10.43IRR51 pKa = 11.84KK52 pKa = 8.74NFLNEE57 pKa = 4.89FYY59 pKa = 9.98MAWAMAGTYY68 pKa = 10.07SAIVKK73 pKa = 7.43TNPSLSALDD82 pKa = 3.47ILSDD86 pKa = 3.63IEE88 pKa = 4.02IEE90 pKa = 4.35KK91 pKa = 10.82VGSCIMSDD99 pKa = 3.33EE100 pKa = 4.83GIIGCILLDD109 pKa = 4.22DD110 pKa = 4.51PRR112 pKa = 11.84AINTFKK118 pKa = 10.81DD119 pKa = 3.44LCSGRR124 pKa = 11.84GLFEE128 pKa = 3.85IVADD132 pKa = 4.24HH133 pKa = 5.85YY134 pKa = 11.74SEE136 pKa = 5.11VNMHH140 pKa = 6.26EE141 pKa = 4.43VSSMAPFCKK150 pKa = 10.22DD151 pKa = 3.03KK152 pKa = 11.43NDD154 pKa = 3.67AKK156 pKa = 11.05SWTNWTSTSRR166 pKa = 11.84SRR168 pKa = 11.84PNSVKK173 pKa = 10.47INFEE177 pKa = 3.92PLEE180 pKa = 4.58SINSFTVIMEE190 pKa = 4.1IRR192 pKa = 11.84AKK194 pKa = 10.63LDD196 pKa = 3.57LVSGASFDD204 pKa = 5.92LDD206 pKa = 3.23ILDD209 pKa = 4.13KK210 pKa = 11.45GKK212 pKa = 10.35VCSPVDD218 pKa = 3.43IRR220 pKa = 11.84PKK222 pKa = 10.29AKK224 pKa = 9.73VSEE227 pKa = 4.31VQEE230 pKa = 4.43VPTTSYY236 pKa = 10.87LPSAPSPSVTQPAVQNRR253 pKa = 11.84QITNNDD259 pKa = 3.16FQKK262 pKa = 10.82EE263 pKa = 4.09FVPTGHH269 pKa = 5.16VTEE272 pKa = 4.6VEE274 pKa = 4.35SFFNYY279 pKa = 9.25IMEE282 pKa = 4.3VRR284 pKa = 11.84ASPSTLGCGLMKK296 pKa = 10.38YY297 pKa = 10.01SPEE300 pKa = 4.53FIWSFALSNCVQGHH314 pKa = 5.41APSWARR320 pKa = 11.84NAPVLEE326 pKa = 4.97LISRR330 pKa = 11.84LNDD333 pKa = 3.27YY334 pKa = 10.16CIHH337 pKa = 6.36SIKK340 pKa = 10.77DD341 pKa = 2.92EE342 pKa = 4.1GMYY345 pKa = 10.13SRR347 pKa = 11.84EE348 pKa = 3.74MLGTKK353 pKa = 9.53TYY355 pKa = 10.79VDD357 pKa = 3.76SSTLSEE363 pKa = 4.08TMEE366 pKa = 4.41SLKK369 pKa = 10.77SSQKK373 pKa = 10.13RR374 pKa = 11.84EE375 pKa = 3.84SKK377 pKa = 10.75AILNVEE383 pKa = 4.2SMMKK387 pKa = 10.22EE388 pKa = 3.69LLAHH392 pKa = 6.54IKK394 pKa = 10.29SGEE397 pKa = 3.93RR398 pKa = 11.84SIPMAALPTVSAEE411 pKa = 4.05DD412 pKa = 3.51KK413 pKa = 10.74PLFEE417 pKa = 6.99AEE419 pKa = 4.33GQRR422 pKa = 11.84NPQVTKK428 pKa = 9.78EE429 pKa = 3.8PTGIVFIPPPPVV441 pKa = 2.91

Molecular weight: 48.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9U5H2|A0A1C9U5H2_9MONO Putative glycoprotein OS=Bolahun virus variant 2 OX=1903560 PE=4 SV=1
MM1 pKa = 7.7AFYY4 pKa = 10.11FGPVIDD10 pKa = 4.08WQDD13 pKa = 3.25FLSFYY18 pKa = 9.83WLHH21 pKa = 6.44CFFVVIIIFILGLVVSLLRR40 pKa = 11.84GLTRR44 pKa = 11.84EE45 pKa = 3.92VRR47 pKa = 11.84EE48 pKa = 4.23PAWYY52 pKa = 8.97AQWVRR57 pKa = 11.84EE58 pKa = 4.24SKK60 pKa = 10.17SRR62 pKa = 11.84KK63 pKa = 8.49RR64 pKa = 3.52

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3647

40

2018

607.8

68.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.182 ± 0.68

2.468 ± 0.356

5.786 ± 0.377

6.307 ± 0.337

4.442 ± 0.275

5.84 ± 0.35

2.358 ± 0.447

6.828 ± 0.453

6.032 ± 0.247

9.158 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.852 ± 0.282

4.552 ± 0.355

4.415 ± 0.636

2.906 ± 0.214

5.758 ± 0.784

8.199 ± 0.615

5.621 ± 0.716

6.252 ± 0.953

1.069 ± 0.185

3.976 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski