Amino acid dipepetide frequency for Bacillus phage vB_BpsM-61

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.231AlaAla: 3.231 ± 0.521
0.269AlaCys: 0.269 ± 0.114
2.356AlaAsp: 2.356 ± 0.401
4.241AlaGlu: 4.241 ± 0.462
2.626AlaPhe: 2.626 ± 0.42
3.501AlaGly: 3.501 ± 0.592
0.606AlaHis: 0.606 ± 0.188
4.039AlaIle: 4.039 ± 0.528
3.501AlaLys: 3.501 ± 0.421
4.78AlaLeu: 4.78 ± 0.695
2.087AlaMet: 2.087 ± 0.406
3.501AlaAsn: 3.501 ± 0.472
1.548AlaPro: 1.548 ± 0.29
2.154AlaGln: 2.154 ± 0.29
1.952AlaArg: 1.952 ± 0.341
3.905AlaSer: 3.905 ± 0.592
3.029AlaThr: 3.029 ± 0.49
3.77AlaVal: 3.77 ± 0.418
0.673AlaTrp: 0.673 ± 0.226
2.356AlaTyr: 2.356 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
0.135CysAla: 0.135 ± 0.083
0.067CysCys: 0.067 ± 0.072
0.404CysAsp: 0.404 ± 0.184
0.673CysGlu: 0.673 ± 0.232
0.202CysPhe: 0.202 ± 0.113
0.404CysGly: 0.404 ± 0.182
0.135CysHis: 0.135 ± 0.104
0.337CysIle: 0.337 ± 0.151
0.404CysLys: 0.404 ± 0.154
0.404CysLeu: 0.404 ± 0.195
0.269CysMet: 0.269 ± 0.148
0.135CysAsn: 0.135 ± 0.088
0.337CysPro: 0.337 ± 0.139
0.135CysGln: 0.135 ± 0.097
0.202CysArg: 0.202 ± 0.131
0.337CysSer: 0.337 ± 0.13
0.202CysThr: 0.202 ± 0.106
0.337CysVal: 0.337 ± 0.157
0.067CysTrp: 0.067 ± 0.072
0.269CysTyr: 0.269 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
4.309AspAla: 4.309 ± 0.604
0.202AspCys: 0.202 ± 0.118
6.53AspAsp: 6.53 ± 0.893
5.588AspGlu: 5.588 ± 0.762
2.962AspPhe: 2.962 ± 0.347
5.453AspGly: 5.453 ± 0.542
1.952AspHis: 1.952 ± 0.38
4.511AspIle: 4.511 ± 0.575
4.578AspLys: 4.578 ± 0.709
4.511AspLeu: 4.511 ± 0.552
2.289AspMet: 2.289 ± 0.366
2.895AspAsn: 2.895 ± 0.368
3.905AspPro: 3.905 ± 0.427
2.76AspGln: 2.76 ± 0.511
3.366AspArg: 3.366 ± 0.613
2.828AspSer: 2.828 ± 0.391
2.76AspThr: 2.76 ± 0.435
4.241AspVal: 4.241 ± 0.473
0.808AspTrp: 0.808 ± 0.238
2.962AspTyr: 2.962 ± 0.543
0.0AspXaa: 0.0 ± 0.0
Glu
2.491GluAla: 2.491 ± 0.392
0.606GluCys: 0.606 ± 0.277
2.626GluAsp: 2.626 ± 0.394
6.194GluGlu: 6.194 ± 0.999
3.164GluPhe: 3.164 ± 0.423
6.261GluGly: 6.261 ± 0.695
1.414GluHis: 1.414 ± 0.36
6.396GluIle: 6.396 ± 0.743
6.194GluLys: 6.194 ± 0.812
6.261GluLeu: 6.261 ± 0.624
2.424GluMet: 2.424 ± 0.451
3.231GluAsn: 3.231 ± 0.415
1.818GluPro: 1.818 ± 0.383
3.972GluGln: 3.972 ± 0.436
4.78GluArg: 4.78 ± 0.601
5.116GluSer: 5.116 ± 0.566
4.241GluThr: 4.241 ± 0.453
3.703GluVal: 3.703 ± 0.481
1.818GluTrp: 1.818 ± 0.302
2.356GluTyr: 2.356 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
1.818PheAla: 1.818 ± 0.281
0.067PheCys: 0.067 ± 0.072
2.76PheAsp: 2.76 ± 0.387
2.222PheGlu: 2.222 ± 0.342
1.414PhePhe: 1.414 ± 0.369
2.356PheGly: 2.356 ± 0.45
0.875PheHis: 0.875 ± 0.202
2.76PheIle: 2.76 ± 0.453
2.693PheLys: 2.693 ± 0.413
2.626PheLeu: 2.626 ± 0.433
1.75PheMet: 1.75 ± 0.319
2.491PheAsn: 2.491 ± 0.377
1.346PhePro: 1.346 ± 0.318
1.212PheGln: 1.212 ± 0.275
1.414PheArg: 1.414 ± 0.263
2.424PheSer: 2.424 ± 0.498
2.424PheThr: 2.424 ± 0.465
1.75PheVal: 1.75 ± 0.331
0.404PheTrp: 0.404 ± 0.172
1.212PheTyr: 1.212 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
4.241GlyAla: 4.241 ± 0.644
0.135GlyCys: 0.135 ± 0.091
5.386GlyAsp: 5.386 ± 0.7
4.915GlyGlu: 4.915 ± 0.699
2.02GlyPhe: 2.02 ± 0.335
5.857GlyGly: 5.857 ± 0.771
1.346GlyHis: 1.346 ± 0.258
5.992GlyIle: 5.992 ± 0.71
4.847GlyLys: 4.847 ± 0.541
4.443GlyLeu: 4.443 ± 0.696
3.029GlyMet: 3.029 ± 0.39
3.635GlyAsn: 3.635 ± 0.599
2.154GlyPro: 2.154 ± 0.391
3.097GlyGln: 3.097 ± 0.495
3.837GlyArg: 3.837 ± 0.537
5.588GlySer: 5.588 ± 0.694
4.376GlyThr: 4.376 ± 0.541
5.184GlyVal: 5.184 ± 0.545
1.077GlyTrp: 1.077 ± 0.34
2.962GlyTyr: 2.962 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
1.01HisAla: 1.01 ± 0.296
0.202HisCys: 0.202 ± 0.12
1.616HisAsp: 1.616 ± 0.334
1.414HisGlu: 1.414 ± 0.284
0.606HisPhe: 0.606 ± 0.222
1.077HisGly: 1.077 ± 0.236
0.606HisHis: 0.606 ± 0.195
1.683HisIle: 1.683 ± 0.364
1.481HisLys: 1.481 ± 0.357
1.414HisLeu: 1.414 ± 0.36
0.808HisMet: 0.808 ± 0.215
0.673HisAsn: 0.673 ± 0.219
0.539HisPro: 0.539 ± 0.187
0.943HisGln: 0.943 ± 0.291
1.414HisArg: 1.414 ± 0.3
1.212HisSer: 1.212 ± 0.295
0.943HisThr: 0.943 ± 0.231
1.818HisVal: 1.818 ± 0.336
0.202HisTrp: 0.202 ± 0.113
1.01HisTyr: 1.01 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
3.972IleAla: 3.972 ± 0.465
0.202IleCys: 0.202 ± 0.109
5.992IleAsp: 5.992 ± 0.656
6.598IleGlu: 6.598 ± 0.612
1.952IlePhe: 1.952 ± 0.324
4.713IleGly: 4.713 ± 0.627
1.279IleHis: 1.279 ± 0.325
4.578IleIle: 4.578 ± 0.607
4.915IleLys: 4.915 ± 0.668
4.713IleLeu: 4.713 ± 0.545
2.558IleMet: 2.558 ± 0.362
3.299IleAsn: 3.299 ± 0.431
2.76IlePro: 2.76 ± 0.363
3.097IleGln: 3.097 ± 0.436
5.386IleArg: 5.386 ± 0.656
3.231IleSer: 3.231 ± 0.56
5.049IleThr: 5.049 ± 0.582
4.376IleVal: 4.376 ± 0.667
0.606IleTrp: 0.606 ± 0.214
2.289IleTyr: 2.289 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
3.366LysAla: 3.366 ± 0.531
0.269LysCys: 0.269 ± 0.123
4.713LysAsp: 4.713 ± 0.62
6.463LysGlu: 6.463 ± 1.018
2.289LysPhe: 2.289 ± 0.367
5.992LysGly: 5.992 ± 0.721
1.212LysHis: 1.212 ± 0.259
3.837LysIle: 3.837 ± 0.605
7.473LysLys: 7.473 ± 1.076
4.982LysLeu: 4.982 ± 0.57
3.501LysMet: 3.501 ± 0.508
3.635LysAsn: 3.635 ± 0.46
2.626LysPro: 2.626 ± 0.456
2.76LysGln: 2.76 ± 0.42
4.578LysArg: 4.578 ± 0.602
4.578LysSer: 4.578 ± 0.672
3.972LysThr: 3.972 ± 0.697
4.443LysVal: 4.443 ± 0.567
1.548LysTrp: 1.548 ± 0.3
2.626LysTyr: 2.626 ± 0.436
0.0LysXaa: 0.0 ± 0.0
Leu
4.174LeuAla: 4.174 ± 0.593
0.471LeuCys: 0.471 ± 0.197
5.386LeuAsp: 5.386 ± 0.561
4.645LeuGlu: 4.645 ± 0.61
2.558LeuPhe: 2.558 ± 0.336
4.241LeuGly: 4.241 ± 0.519
1.144LeuHis: 1.144 ± 0.279
4.309LeuIle: 4.309 ± 0.538
6.261LeuLys: 6.261 ± 0.866
3.837LeuLeu: 3.837 ± 0.542
3.164LeuMet: 3.164 ± 0.427
3.501LeuAsn: 3.501 ± 0.568
2.289LeuPro: 2.289 ± 0.463
2.626LeuGln: 2.626 ± 0.479
3.164LeuArg: 3.164 ± 0.562
4.241LeuSer: 4.241 ± 0.579
3.905LeuThr: 3.905 ± 0.472
4.713LeuVal: 4.713 ± 0.414
0.808LeuTrp: 0.808 ± 0.204
1.952LeuTyr: 1.952 ± 0.345
0.0LeuXaa: 0.0 ± 0.0
Met
2.289MetAla: 2.289 ± 0.337
0.135MetCys: 0.135 ± 0.1
2.828MetAsp: 2.828 ± 0.467
2.356MetGlu: 2.356 ± 0.361
1.279MetPhe: 1.279 ± 0.328
2.424MetGly: 2.424 ± 0.325
0.808MetHis: 0.808 ± 0.225
3.366MetIle: 3.366 ± 0.466
3.366MetLys: 3.366 ± 0.407
1.616MetLeu: 1.616 ± 0.323
1.818MetMet: 1.818 ± 0.373
2.02MetAsn: 2.02 ± 0.339
1.414MetPro: 1.414 ± 0.283
1.346MetGln: 1.346 ± 0.31
1.279MetArg: 1.279 ± 0.269
1.952MetSer: 1.952 ± 0.355
2.693MetThr: 2.693 ± 0.415
2.356MetVal: 2.356 ± 0.364
0.606MetTrp: 0.606 ± 0.205
0.741MetTyr: 0.741 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
3.568AsnAla: 3.568 ± 0.433
0.539AsnCys: 0.539 ± 0.174
2.962AsnAsp: 2.962 ± 0.432
2.962AsnGlu: 2.962 ± 0.477
1.01AsnPhe: 1.01 ± 0.302
4.982AsnGly: 4.982 ± 0.609
1.077AsnHis: 1.077 ± 0.306
3.905AsnIle: 3.905 ± 0.486
3.837AsnLys: 3.837 ± 0.567
4.241AsnLeu: 4.241 ± 0.393
1.279AsnMet: 1.279 ± 0.225
2.626AsnAsn: 2.626 ± 0.401
2.491AsnPro: 2.491 ± 0.352
2.962AsnGln: 2.962 ± 0.445
3.231AsnArg: 3.231 ± 0.466
2.491AsnSer: 2.491 ± 0.46
2.626AsnThr: 2.626 ± 0.476
2.356AsnVal: 2.356 ± 0.391
1.077AsnTrp: 1.077 ± 0.309
1.077AsnTyr: 1.077 ± 0.227
0.0AsnXaa: 0.0 ± 0.0
Pro
1.414ProAla: 1.414 ± 0.278
0.337ProCys: 0.337 ± 0.212
2.693ProAsp: 2.693 ± 0.377
2.76ProGlu: 2.76 ± 0.496
1.548ProPhe: 1.548 ± 0.303
4.107ProGly: 4.107 ± 0.489
0.943ProHis: 0.943 ± 0.218
1.75ProIle: 1.75 ± 0.381
2.356ProLys: 2.356 ± 0.366
2.491ProLeu: 2.491 ± 0.375
1.212ProMet: 1.212 ± 0.242
1.616ProAsn: 1.616 ± 0.353
1.279ProPro: 1.279 ± 0.321
1.077ProGln: 1.077 ± 0.28
1.548ProArg: 1.548 ± 0.36
2.491ProSer: 2.491 ± 0.384
1.952ProThr: 1.952 ± 0.313
2.626ProVal: 2.626 ± 0.402
0.067ProTrp: 0.067 ± 0.06
1.414ProTyr: 1.414 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
3.231GlnAla: 3.231 ± 0.45
0.067GlnCys: 0.067 ± 0.057
1.75GlnAsp: 1.75 ± 0.31
2.895GlnGlu: 2.895 ± 0.524
1.346GlnPhe: 1.346 ± 0.295
2.895GlnGly: 2.895 ± 0.409
0.741GlnHis: 0.741 ± 0.223
3.029GlnIle: 3.029 ± 0.476
2.626GlnLys: 2.626 ± 0.399
3.703GlnLeu: 3.703 ± 0.458
1.346GlnMet: 1.346 ± 0.305
2.154GlnAsn: 2.154 ± 0.365
1.414GlnPro: 1.414 ± 0.346
1.818GlnGln: 1.818 ± 0.328
2.693GlnArg: 2.693 ± 0.49
1.952GlnSer: 1.952 ± 0.363
2.558GlnThr: 2.558 ± 0.448
2.222GlnVal: 2.222 ± 0.422
0.337GlnTrp: 0.337 ± 0.133
1.346GlnTyr: 1.346 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
2.356ArgAla: 2.356 ± 0.405
0.337ArgCys: 0.337 ± 0.137
3.77ArgAsp: 3.77 ± 0.49
4.443ArgGlu: 4.443 ± 0.583
1.818ArgPhe: 1.818 ± 0.335
3.568ArgGly: 3.568 ± 0.54
0.875ArgHis: 0.875 ± 0.319
4.78ArgIle: 4.78 ± 0.51
3.837ArgLys: 3.837 ± 0.44
3.299ArgLeu: 3.299 ± 0.448
2.424ArgMet: 2.424 ± 0.469
2.558ArgAsn: 2.558 ± 0.523
2.222ArgPro: 2.222 ± 0.308
2.154ArgGln: 2.154 ± 0.449
2.962ArgArg: 2.962 ± 0.53
3.837ArgSer: 3.837 ± 0.587
2.895ArgThr: 2.895 ± 0.496
3.097ArgVal: 3.097 ± 0.492
1.414ArgTrp: 1.414 ± 0.291
2.356ArgTyr: 2.356 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
2.895SerAla: 2.895 ± 0.353
0.269SerCys: 0.269 ± 0.126
3.568SerAsp: 3.568 ± 0.47
3.972SerGlu: 3.972 ± 0.509
2.693SerPhe: 2.693 ± 0.457
5.116SerGly: 5.116 ± 0.543
1.481SerHis: 1.481 ± 0.255
4.511SerIle: 4.511 ± 0.427
3.703SerLys: 3.703 ± 0.565
4.174SerLeu: 4.174 ± 0.527
1.683SerMet: 1.683 ± 0.236
3.635SerAsn: 3.635 ± 0.409
2.222SerPro: 2.222 ± 0.383
2.356SerGln: 2.356 ± 0.401
3.905SerArg: 3.905 ± 0.51
3.77SerSer: 3.77 ± 0.642
3.77SerThr: 3.77 ± 0.475
3.433SerVal: 3.433 ± 0.43
1.077SerTrp: 1.077 ± 0.276
2.154SerTyr: 2.154 ± 0.355
0.0SerXaa: 0.0 ± 0.0
Thr
2.76ThrAla: 2.76 ± 0.532
0.202ThrCys: 0.202 ± 0.107
4.107ThrAsp: 4.107 ± 0.416
3.635ThrGlu: 3.635 ± 0.474
2.626ThrPhe: 2.626 ± 0.356
4.713ThrGly: 4.713 ± 0.734
1.077ThrHis: 1.077 ± 0.296
4.241ThrIle: 4.241 ± 0.45
4.309ThrLys: 4.309 ± 0.569
3.635ThrLeu: 3.635 ± 0.325
1.75ThrMet: 1.75 ± 0.343
2.558ThrAsn: 2.558 ± 0.401
1.683ThrPro: 1.683 ± 0.294
1.414ThrGln: 1.414 ± 0.225
3.097ThrArg: 3.097 ± 0.462
3.77ThrSer: 3.77 ± 0.415
3.568ThrThr: 3.568 ± 0.458
5.318ThrVal: 5.318 ± 0.571
1.144ThrTrp: 1.144 ± 0.272
2.558ThrTyr: 2.558 ± 0.506
0.0ThrXaa: 0.0 ± 0.0
Val
4.039ValAla: 4.039 ± 0.623
0.471ValCys: 0.471 ± 0.186
5.52ValAsp: 5.52 ± 0.529
4.982ValGlu: 4.982 ± 0.619
1.683ValPhe: 1.683 ± 0.29
4.174ValGly: 4.174 ± 0.537
1.616ValHis: 1.616 ± 0.346
4.376ValIle: 4.376 ± 0.649
4.174ValLys: 4.174 ± 0.583
3.164ValLeu: 3.164 ± 0.501
2.02ValMet: 2.02 ± 0.345
4.039ValAsn: 4.039 ± 0.64
2.356ValPro: 2.356 ± 0.306
2.356ValGln: 2.356 ± 0.385
3.164ValArg: 3.164 ± 0.513
3.703ValSer: 3.703 ± 0.448
4.039ValThr: 4.039 ± 0.485
3.164ValVal: 3.164 ± 0.49
0.606ValTrp: 0.606 ± 0.145
2.895ValTyr: 2.895 ± 0.495
0.0ValXaa: 0.0 ± 0.0
Trp
0.875TrpAla: 0.875 ± 0.223
0.202TrpCys: 0.202 ± 0.102
0.741TrpAsp: 0.741 ± 0.24
1.683TrpGlu: 1.683 ± 0.329
0.943TrpPhe: 0.943 ± 0.264
0.741TrpGly: 0.741 ± 0.281
0.202TrpHis: 0.202 ± 0.102
0.943TrpIle: 0.943 ± 0.243
1.481TrpLys: 1.481 ± 0.301
0.471TrpLeu: 0.471 ± 0.203
0.471TrpMet: 0.471 ± 0.141
0.943TrpAsn: 0.943 ± 0.202
0.0TrpPro: 0.0 ± 0.0
0.404TrpGln: 0.404 ± 0.147
0.741TrpArg: 0.741 ± 0.182
0.943TrpSer: 0.943 ± 0.299
1.279TrpThr: 1.279 ± 0.292
1.346TrpVal: 1.346 ± 0.3
0.135TrpTrp: 0.135 ± 0.091
0.539TrpTyr: 0.539 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.952TyrAla: 1.952 ± 0.41
0.404TyrCys: 0.404 ± 0.173
3.703TyrAsp: 3.703 ± 0.437
2.424TyrGlu: 2.424 ± 0.401
1.279TyrPhe: 1.279 ± 0.29
1.548TyrGly: 1.548 ± 0.295
1.279TyrHis: 1.279 ± 0.376
2.222TyrIle: 2.222 ± 0.366
2.828TyrLys: 2.828 ± 0.494
2.626TyrLeu: 2.626 ± 0.392
0.741TyrMet: 0.741 ± 0.241
2.222TyrAsn: 2.222 ± 0.366
1.279TyrPro: 1.279 ± 0.31
1.481TyrGln: 1.481 ± 0.308
2.289TyrArg: 2.289 ± 0.433
2.02TyrSer: 2.02 ± 0.379
1.885TyrThr: 1.885 ± 0.426
2.289TyrVal: 2.289 ± 0.336
0.606TyrTrp: 0.606 ± 0.205
1.279TyrTyr: 1.279 ± 0.337
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14855 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski