Hainan oligodon formosanus arterivirus
Average proteome isoelectric point is 7.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GNC3|A0A2P1GNC3_9NIDO Uncharacterized protein OS=Hainan oligodon formosanus arterivirus OX=2116440 PE=4 SV=1
MM1 pKa = 7.55 NPLLLLLPVSLVLANSDD18 pKa = 3.81 MYY20 pKa = 10.75 TPKK23 pKa = 9.97 CVCMATAYY31 pKa = 10.27 EE32 pKa = 4.49 NDD34 pKa = 4.4 LGWFPKK40 pKa = 9.93 PDD42 pKa = 4.03 SDD44 pKa = 4.79 PAACFPISDD53 pKa = 4.15 YY54 pKa = 10.25 QTMVVWPNGMVKK66 pKa = 10.31 CVYY69 pKa = 10.55 NKK71 pKa = 10.46 LLEE74 pKa = 4.1 SHH76 pKa = 7.02 INCTMVTFGKK86 pKa = 9.71 MGLDD90 pKa = 3.39
Molecular weight: 10.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.58
IPC2_protein 4.749
IPC_protein 4.495
Toseland 4.317
ProMoST 4.457
Dawson 4.495
Bjellqvist 4.749
Wikipedia 4.457
Rodwell 4.342
Grimsley 4.228
Solomon 4.482
Lehninger 4.444
Nozaki 4.622
DTASelect 4.876
Thurlkill 4.38
EMBOSS 4.469
Sillero 4.635
Patrickios 0.362
IPC_peptide 4.482
IPC2_peptide 4.609
IPC2.peptide.svr19 4.651
Protein with the highest isoelectric point:
>tr|A0A2P1GNC3|A0A2P1GNC3_9NIDO Uncharacterized protein OS=Hainan oligodon formosanus arterivirus OX=2116440 PE=4 SV=1
MM1 pKa = 7.92 GSQCQEE7 pKa = 3.84 TPLFTRR13 pKa = 11.84 TMNQYY18 pKa = 9.35 RR19 pKa = 11.84 QQPSRR24 pKa = 11.84 RR25 pKa = 11.84 GSFAPRR31 pKa = 11.84 RR32 pKa = 11.84 SNQGRR37 pKa = 11.84 SFMGPEE43 pKa = 3.62 RR44 pKa = 11.84 DD45 pKa = 3.52 LNQLLRR51 pKa = 11.84 DD52 pKa = 3.75 LSFVDD57 pKa = 3.5 RR58 pKa = 11.84 RR59 pKa = 11.84 PVQRR63 pKa = 11.84 QRR65 pKa = 11.84 NPSVRR70 pKa = 11.84 APTNSEE76 pKa = 3.73 TDD78 pKa = 3.33 LLAAIEE84 pKa = 4.03 KK85 pKa = 9.01 HH86 pKa = 6.13 FNVIAPVVAYY96 pKa = 9.45 PEE98 pKa = 4.4 GDD100 pKa = 3.22 ARR102 pKa = 11.84 RR103 pKa = 11.84 AMNKK107 pKa = 9.64 RR108 pKa = 11.84 QLCKK112 pKa = 9.5 TAIMLLRR119 pKa = 11.84 TYY121 pKa = 10.02 FAAAGYY127 pKa = 10.53 LEE129 pKa = 5.29 DD130 pKa = 5.98 KK131 pKa = 10.38 GGHH134 pKa = 5.41 WEE136 pKa = 3.86 AAFLISKK143 pKa = 9.71 SDD145 pKa = 3.65 KK146 pKa = 9.39 PQHH149 pKa = 5.87 EE150 pKa = 4.64 PAVAAAPSQSSASQQ164 pKa = 2.94
Molecular weight: 18.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.414
IPC_protein 9.984
Toseland 10.277
ProMoST 9.984
Dawson 10.438
Bjellqvist 10.16
Wikipedia 10.643
Rodwell 10.628
Grimsley 10.511
Solomon 10.511
Lehninger 10.482
Nozaki 10.292
DTASelect 10.145
Thurlkill 10.306
EMBOSS 10.687
Sillero 10.365
Patrickios 10.335
IPC_peptide 10.511
IPC2_peptide 9.121
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4907
90
3821
817.8
90.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.235 ± 0.351
3.301 ± 0.384
4.687 ± 0.549
4.585 ± 0.938
5.299 ± 0.407
6.154 ± 0.69
3.077 ± 0.823
5.951 ± 0.489
5.482 ± 0.939
9.252 ± 0.404
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.895 ± 0.604
4.32 ± 0.636
5.482 ± 0.721
3.566 ± 0.529
4.483 ± 0.593
7.764 ± 0.307
6.949 ± 0.941
6.318 ± 0.455
0.958 ± 0.139
3.24 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here