Lactobacillus phage phig1e

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O03941|O03941_9CAUD Holin OS=Lactobacillus phage phig1e OX=52979 GN=Hol PE=4 SV=1
MM1 pKa = 7.9LDD3 pKa = 3.39LLLWLKK9 pKa = 11.01DD10 pKa = 3.8CGCSLDD16 pKa = 4.97EE17 pKa = 4.27ITASASNCVVSGQITKK33 pKa = 10.62DD34 pKa = 3.21DD35 pKa = 3.96YY36 pKa = 11.17KK37 pKa = 10.98TITGEE42 pKa = 4.16DD43 pKa = 4.03YY44 pKa = 11.04VASTTEE50 pKa = 3.58

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O03966|O03966_9CAUD GpG protein OS=Lactobacillus phage phig1e OX=52979 GN=gpG PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.54KK3 pKa = 9.88EE4 pKa = 4.26VNSKK8 pKa = 8.63TRR10 pKa = 11.84DD11 pKa = 3.26LSGRR15 pKa = 11.84QFGHH19 pKa = 5.61LTVLKK24 pKa = 10.79KK25 pKa = 9.75MDD27 pKa = 3.73GRR29 pKa = 11.84SHH31 pKa = 7.29SGDD34 pKa = 4.49DD35 pKa = 3.37MWLCQCTCGNQVQVSTGRR53 pKa = 11.84LNSGGQTTCGDD64 pKa = 4.25RR65 pKa = 11.84YY66 pKa = 10.29RR67 pKa = 11.84HH68 pKa = 5.38PTNHH72 pKa = 7.21KK73 pKa = 10.31YY74 pKa = 11.04GDD76 pKa = 3.37LTGRR80 pKa = 11.84QFGHH84 pKa = 5.88LTVLEE89 pKa = 4.99RR90 pKa = 11.84IPVDD94 pKa = 3.74DD95 pKa = 4.53NHH97 pKa = 6.96PKK99 pKa = 10.56SLWLCEE105 pKa = 4.13CDD107 pKa = 3.37CGKK110 pKa = 10.63QIAVPASPLVKK121 pKa = 10.32GIRR124 pKa = 11.84TSCGHH129 pKa = 5.74GFKK132 pKa = 10.41TSEE135 pKa = 4.18RR136 pKa = 11.84LNALEE141 pKa = 4.11KK142 pKa = 10.67AHH144 pKa = 6.25YY145 pKa = 9.75DD146 pKa = 3.31KK147 pKa = 11.61LFVDD151 pKa = 5.58GVAVGLIDD159 pKa = 4.23SNRR162 pKa = 11.84KK163 pKa = 8.93KK164 pKa = 10.16VAKK167 pKa = 9.89NSKK170 pKa = 8.63TGVTGVSEE178 pKa = 4.52VQRR181 pKa = 11.84KK182 pKa = 8.75NGPKK186 pKa = 9.82YY187 pKa = 10.15LAQIYY192 pKa = 8.77VNHH195 pKa = 5.23VRR197 pKa = 11.84TRR199 pKa = 11.84IGYY202 pKa = 9.34FDD204 pKa = 4.24TIEE207 pKa = 4.02EE208 pKa = 4.06AKK210 pKa = 9.97EE211 pKa = 3.55ARR213 pKa = 11.84LAAEE217 pKa = 5.03LKK219 pKa = 10.24FLPKK223 pKa = 10.29KK224 pKa = 10.09IPTRR228 pKa = 11.84GRR230 pKa = 11.84RR231 pKa = 11.84GG232 pKa = 3.11

Molecular weight: 25.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10712

43

1608

214.2

23.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.122 ± 0.499

0.728 ± 0.169

7.319 ± 0.39

5.489 ± 0.353

3.594 ± 0.223

6.871 ± 0.576

2.007 ± 0.179

5.975 ± 0.256

7.767 ± 0.688

8.066 ± 0.412

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.199

5.274 ± 0.335

3.062 ± 0.156

4.248 ± 0.254

3.855 ± 0.299

6.871 ± 0.262

6.796 ± 0.389

6.012 ± 0.211

1.195 ± 0.11

3.912 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski